FastQCFastQC Report
Thu 2 Feb 2017
SRR4063042_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063042_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752778
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24420.3243984282218662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16690.22171211167170135No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9990.13270844790894526No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9920.13177855888455828No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9490.12606638344903812No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9410.12500365313545295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9220.12247966864068822No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9140.12141693832710308No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9070.12048704930271607No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9030.1199556841459235No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8860.11769738222955506No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8670.11517339773479034No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8360.11105531776964789No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8320.1105239526128553No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7660.10175642752577785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATAC805.2459654E-921.9997713
ATCGTTT1900.021.89305529
CATCGTT1950.021.33169628
GGCATCG2150.020.0914826
TCTAGCG1850.019.89036428
CGGCATC2150.019.34735125
GTATAAT701.7607066E-518.315941
TCAGTAC701.7860859E-518.2855243
GTAGGAC3800.018.1050743
ACCGTCG1600.017.9986158
CTAGCGG2050.017.9498429
GTCCTAA2500.017.9496231
CTATCCC450.00885143317.7764134
GCATCGT2350.017.7007727
TAGGACG3650.017.53294
TAGCGGC2200.017.45320530
TGTAGGA4150.017.3595962
CGTTTAT2400.017.33231
AACGAAT652.1004229E-417.22944631
AGCGGCG2150.017.11496431
Produced by FastQC (version 0.11.5)