##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063042_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752778
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.220036983014914	32.0	32.0	32.0	32.0	32.0
2	30.834915473087683	32.0	32.0	32.0	32.0	32.0
3	30.89214881412581	32.0	32.0	32.0	32.0	32.0
4	30.957605030965304	32.0	32.0	32.0	32.0	32.0
5	30.869188525700803	32.0	32.0	32.0	32.0	32.0
6	34.527928552641015	36.0	36.0	36.0	32.0	36.0
7	34.4484602366169	36.0	36.0	36.0	32.0	36.0
8	34.43506717783995	36.0	36.0	36.0	32.0	36.0
9	34.570485588048534	36.0	36.0	36.0	32.0	36.0
10	34.28432552492235	36.0	36.0	36.0	32.0	36.0
11	34.56815688024889	36.0	36.0	36.0	32.0	36.0
12	34.38040830098648	36.0	36.0	36.0	32.0	36.0
13	34.46670465927538	36.0	36.0	36.0	32.0	36.0
14	34.359520336672965	36.0	36.0	36.0	32.0	36.0
15	34.314111198786364	36.0	36.0	36.0	32.0	36.0
16	34.324730531444864	36.0	36.0	36.0	32.0	36.0
17	34.24663313752527	36.0	36.0	36.0	32.0	36.0
18	34.27685320240496	36.0	36.0	36.0	32.0	36.0
19	34.263818815108834	36.0	36.0	36.0	32.0	36.0
20	34.23646148001137	36.0	36.0	36.0	32.0	36.0
21	34.22078886471177	36.0	36.0	36.0	32.0	36.0
22	34.194419868805944	36.0	36.0	36.0	32.0	36.0
23	34.14976394102909	36.0	36.0	36.0	32.0	36.0
24	34.11532882204315	36.0	36.0	36.0	32.0	36.0
25	34.09451657726448	36.0	36.0	36.0	32.0	36.0
26	34.055432013156604	36.0	36.0	36.0	32.0	36.0
27	34.05241784430469	36.0	36.0	36.0	32.0	36.0
28	34.02644737226646	36.0	36.0	36.0	32.0	36.0
29	33.993880001806644	36.0	36.0	36.0	32.0	36.0
30	33.957029828183074	36.0	36.0	36.0	32.0	36.0
31	33.97415705559939	36.0	36.0	36.0	32.0	36.0
32	33.9300059247215	36.0	36.0	36.0	32.0	36.0
33	33.897000178007325	36.0	36.0	36.0	32.0	36.0
34	33.8883575237321	36.0	36.0	36.0	32.0	36.0
35	33.84542986112772	36.0	36.0	36.0	32.0	36.0
36	33.80637850734214	36.0	36.0	36.0	32.0	36.0
37	33.79588271708259	36.0	36.0	36.0	32.0	36.0
38	33.193543116297235	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	4.0
9	1.0
10	3.0
11	3.0
12	1.0
13	3.0
14	130.0
15	225.0
16	293.0
17	370.0
18	425.0
19	541.0
20	695.0
21	992.0
22	1441.0
23	2189.0
24	3216.0
25	4804.0
26	7092.0
27	10390.0
28	14630.0
29	20043.0
30	26938.0
31	36699.0
32	50195.0
33	75504.0
34	168284.0
35	327666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59694098088113	17.266566916043224	11.34590191188695	25.790590191188695
2	16.601514992417062	19.86689652514066	38.08096529925686	25.450623183185417
3	19.21545494764784	24.207011907940977	28.30763221561039	28.269900928800794
4	12.562520341943925	15.50464626604938	36.22028415619956	35.71254923580713
5	14.383390587928977	36.71587108018566	33.65281663385487	15.247921698030495
6	33.54145190601188	35.71846073732425	17.19130259200612	13.548784764657748
7	29.953850936132564	30.63266992393508	20.948539941390422	18.464939198541934
8	27.951852683345447	32.934098327118534	19.698102880948174	19.415946108587846
9	27.877674427254785	14.25519874385277	18.597249122583285	39.26987770630917
10	16.12847946395598	26.228456349448837	31.497766926062802	26.14529726053238
11	37.31215992178201	21.18581023635436	22.56804221220881	18.93398762965482
12	25.40494152874145	23.69616997647334	28.559187951586356	22.339700543198855
13	29.957432561698873	19.531333035733265	25.150792891609804	25.360441510958058
14	23.823302392254234	20.30114743709821	24.8514389793828	31.02411119126476
15	25.03019423964328	27.592552486158482	22.564032382574638	24.813220891623605
16	25.490928076927677	25.64344426184425	24.475127772161013	24.390499889067062
17	23.761230438907212	25.843229583254722	25.64645608403464	24.749083893803427
18	24.84084894731808	24.40298428150872	27.228911821283198	23.527254949890004
19	25.706690544070014	24.751107296867154	25.76261999590825	23.77958216315458
20	25.825611594896014	23.95167022032694	25.553540707676568	24.66917747710048
21	27.002529398948916	24.072996540674968	24.298702885959177	24.625771174416936
22	25.97544418081288	24.72174765587844	25.042179574723605	24.260628588585078
23	23.869901504140973	24.52605344336953	25.89375626061149	25.71028879187801
24	24.678518427978695	25.217254981154184	25.691688577057924	24.412538013809193
25	24.772576597211796	24.635464897677736	25.632049062605045	24.959909442505424
26	23.85074880690454	25.713200046767216	26.247967220433022	24.18808392589522
27	25.139858514065562	25.093227938490152	25.10491879504467	24.661994752399615
28	23.96801041521365	25.05775528233333	25.94623677340932	25.0279975290437
29	24.13496246245249	25.1329524001844	25.880780589680263	24.851304547682847
30	24.000515434472735	25.29308693440034	26.358362459366365	24.348035171760568
31	24.941148963530956	25.01594159001419	24.894652659322908	25.14825678713195
32	24.27418304908215	25.394360321918118	25.027433183923225	25.304023445076506
33	23.812946295127702	24.86259922282374	25.92845992892491	25.395994553123654
34	24.663978583907372	25.111962853973335	26.096943689758934	24.12711487236035
35	25.556343847733835	24.825889286577866	25.868162118929376	23.749604746758923
36	23.97273741829197	25.667215815485996	25.506988361587922	24.85305840463411
37	25.083098408177257	25.54754610599929	25.015477161554994	24.353878324268454
38	24.165517534560312	25.506481636470397	25.52441606818804	24.80358476078125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	575.0
1	384.5
2	194.0
3	194.0
4	448.0
5	702.0
6	702.0
7	795.0
8	888.0
9	850.0
10	812.0
11	812.0
12	1032.5
13	1253.0
14	1560.0
15	1867.0
16	1867.0
17	2802.0
18	3737.0
19	3737.0
20	4441.5
21	5146.0
22	5239.0
23	5332.0
24	5332.0
25	6403.5
26	7475.0
27	7475.0
28	9887.0
29	12299.0
30	15835.0
31	19371.0
32	19371.0
33	24284.5
34	29198.0
35	29198.0
36	32119.0
37	35040.0
38	39482.0
39	43924.0
40	43924.0
41	45853.5
42	47783.0
43	53136.5
44	58490.0
45	58490.0
46	60938.5
47	63387.0
48	63387.0
49	66004.0
50	68621.0
51	68298.5
52	67976.0
53	67976.0
54	64332.0
55	60688.0
56	60688.0
57	58999.0
58	57310.0
59	51157.5
60	45005.0
61	45005.0
62	42996.0
63	40987.0
64	33848.0
65	26709.0
66	26709.0
67	22409.0
68	18109.0
69	18109.0
70	14710.0
71	11311.0
72	8887.0
73	6463.0
74	6463.0
75	4920.5
76	3378.0
77	3378.0
78	3233.0
79	3088.0
80	2430.5
81	1773.0
82	1773.0
83	1856.0
84	1939.0
85	1939.0
86	1265.5
87	592.0
88	523.5
89	455.0
90	455.0
91	343.5
92	232.0
93	189.5
94	147.0
95	147.0
96	144.5
97	142.0
98	142.0
99	261.0
100	380.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1199556841459235
2	0.0572545956443998
3	0.011557192160238476
4	0.0030553496515572983
5	0.0
6	2.656825783962868E-4
7	0.0
8	6.642064459907171E-4
9	0.0
10	5.313651567925736E-4
11	0.0013284128919814342
12	0.0019926193379721513
13	0.011955716027832906
14	0.007571953484294174
15	0.020059034668919656
16	0.009431731533068182
17	0.018066415330947504
18	0.004516603832736876
19	0.00584501672471831
20	0.0043837625435387325
21	0.003985238675944303
22	0.00584501672471831
23	0.007439112195096032
24	0.01208855731703105
25	0.014214017944201346
26	0.015143906968588349
27	0.007040588327501601
28	0.0043837625435387325
29	0.00863468379787932
30	0.0019926193379721513
31	0.003985238675944303
32	0.005313651567925737
33	0.007040588327501601
34	0.00969741411146447
35	0.012752763763021768
36	0.013018446341418055
37	0.007439112195096032
38	0.004782286411133162
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	752778.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.74495272504679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69516124091656	48.579233376877646
2	11.300322432474086	13.276738141469519
3	2.786781904676902	4.911281137357813
4	1.082542529294107	2.5437563882493954
5	0.5110182550848964	1.500987161829907
6	0.31168109165963254	1.0985814596901653
7	0.2003153245647812	0.8237259990162332
8	0.14676612721269047	0.6897415363798163
9	0.12186615915887164	0.6443119582713848
>10	0.6522333389081808	7.525950705612719
>50	0.09157593796948278	3.783442762796617
>100	0.09066878007307337	10.524568084341038
>500	0.008613534106445461	3.5502673936090376
>1k	4.533439003392348E-4	0.5474138944987905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2442	0.3243984282218662	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1669	0.22171211167170135	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	999	0.13270844790894526	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	992	0.13177855888455828	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	949	0.12606638344903812	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	941	0.12500365313545295	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	922	0.12247966864068822	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	914	0.12141693832710308	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	907	0.12048704930271607	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	903	0.1199556841459235	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	886	0.11769738222955506	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	867	0.11517339773479034	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	836	0.11105531776964789	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	832	0.1105239526128553	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	766	0.10175642752577785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	6.642064459907171E-4
13	0.0	0.0	0.0	0.0	7.970477351888605E-4
14	0.0	0.0	0.0	0.0	7.970477351888605E-4
15	0.0	0.0	0.0	0.0	0.0010627303135851472
16	0.0	0.0	0.0	1.328412891981434E-4	0.0011955716027832906
17	0.0	0.0	0.0	1.328412891981434E-4	0.0011955716027832906
18	0.0	0.0	0.0	2.656825783962868E-4	0.0011955716027832906
19	1.328412891981434E-4	0.0	0.0	2.656825783962868E-4	0.0013284128919814342
20	1.328412891981434E-4	0.0	0.0	2.656825783962868E-4	0.0014612541811795776
21	1.328412891981434E-4	0.0	0.0	2.656825783962868E-4	0.001594095470377721
22	1.328412891981434E-4	0.0	0.0	5.313651567925736E-4	0.001594095470377721
23	1.328412891981434E-4	0.0	0.0	0.001594095470377721	0.001594095470377721
24	1.328412891981434E-4	0.0	0.0	0.0026568257839628684	0.001594095470377721
25	1.328412891981434E-4	0.0	0.0	0.003985238675944303	0.001594095470377721
26	1.328412891981434E-4	0.0	0.0	0.004915127700331306	0.001594095470377721
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTATAC	80	5.2459654E-9	21.999771	3
ATCGTTT	190	0.0	21.893055	29
CATCGTT	195	0.0	21.331696	28
GGCATCG	215	0.0	20.09148	26
TCTAGCG	185	0.0	19.890364	28
CGGCATC	215	0.0	19.347351	25
GTATAAT	70	1.7607066E-5	18.31594	1
TCAGTAC	70	1.7860859E-5	18.285524	3
GTAGGAC	380	0.0	18.105074	3
ACCGTCG	160	0.0	17.998615	8
CTAGCGG	205	0.0	17.94984	29
GTCCTAA	250	0.0	17.949623	1
CTATCCC	45	0.008851433	17.776413	4
GCATCGT	235	0.0	17.70077	27
TAGGACG	365	0.0	17.5329	4
TAGCGGC	220	0.0	17.453205	30
TGTAGGA	415	0.0	17.359596	2
CGTTTAT	240	0.0	17.332	31
AACGAAT	65	2.1004229E-4	17.229446	31
AGCGGCG	215	0.0	17.114964	31
>>END_MODULE