FastQCFastQC Report
Thu 2 Feb 2017
SRR4063042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752778
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30480.4049002494759411No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21380.2840146763056306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12970.17229515208999202No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10690.1420073381528153No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10530.13988187752564502No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10100.13416970209012483No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9930.1319114001737564No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9770.12978593954658613No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.1218154621946975No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9160.12168262090549936No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC8820.11716601707276249No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8700.11557192160238476No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8540.11344646097521448No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8380.11132100034804418No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8150.10826565069648687No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8050.10693723780450545No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC7750.10295199912856114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA350.002064660422.8594689
TACGCCT951.718945E-920.2125825
CATTCCG400.0044794320.0020339
ATAAGAT659.423855E-619.6930033
GATATAC902.0347215E-819.5562461
ACCGTCC507.1878626E-419.2019528
CACCGTC601.1366326E-418.6685647
GTACGCC802.7926417E-618.0006334
TGCGTAG802.7944134E-617.9994413
GAGTAGT450.008845719517.7784041
TCTAGCG2600.017.23023228
TAACGAA1404.0017767E-1117.14232313
TAGTACG854.9808023E-616.9406472
ACGCCTG957.675553E-716.8438196
TGAACGG600.00243919916.0016275
TAGAACC1900.016.0005654
CTAGTAC1203.1035597E-816.0005651
CTAGCGG2700.015.99950329
ACGTTTT1900.015.99950229
AACCCGT703.6951562E-415.99950229
Produced by FastQC (version 0.11.5)