##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063042_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752778
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19623448081639	32.0	32.0	32.0	32.0	32.0
2	31.301483305835188	32.0	32.0	32.0	32.0	32.0
3	31.379916788216445	32.0	32.0	32.0	32.0	32.0
4	31.479323253336307	32.0	32.0	32.0	32.0	32.0
5	31.394791027367962	32.0	32.0	32.0	32.0	32.0
6	34.92034570617101	36.0	36.0	36.0	36.0	36.0
7	34.95974776096007	36.0	36.0	36.0	36.0	36.0
8	34.894470879861	36.0	36.0	36.0	36.0	36.0
9	35.00129520256968	36.0	36.0	36.0	36.0	36.0
10	34.84660417812423	36.0	36.0	36.0	32.0	36.0
11	35.02842537906262	36.0	36.0	36.0	36.0	36.0
12	34.91717212777206	36.0	36.0	36.0	32.0	36.0
13	34.97137137376491	36.0	36.0	36.0	36.0	36.0
14	34.917857588824326	36.0	36.0	36.0	32.0	36.0
15	34.88046276591505	36.0	36.0	36.0	32.0	36.0
16	34.89242645242024	36.0	36.0	36.0	32.0	36.0
17	34.86570808392381	36.0	36.0	36.0	32.0	36.0
18	34.86669642311545	36.0	36.0	36.0	32.0	36.0
19	34.850423365188675	36.0	36.0	36.0	32.0	36.0
20	34.83503635866085	36.0	36.0	36.0	32.0	36.0
21	34.81470898458775	36.0	36.0	36.0	32.0	36.0
22	34.80009511436307	36.0	36.0	36.0	32.0	36.0
23	34.744127750810996	36.0	36.0	36.0	32.0	36.0
24	34.72409262757413	36.0	36.0	36.0	32.0	36.0
25	34.695744296459246	36.0	36.0	36.0	32.0	36.0
26	34.629691622231256	36.0	36.0	36.0	32.0	36.0
27	34.62237339560933	36.0	36.0	36.0	32.0	36.0
28	34.576572375919596	36.0	36.0	36.0	32.0	36.0
29	34.55010374904686	36.0	36.0	36.0	32.0	36.0
30	34.516783168477296	36.0	36.0	36.0	32.0	36.0
31	34.50820693484666	36.0	36.0	36.0	32.0	36.0
32	34.48444029979622	36.0	36.0	36.0	32.0	36.0
33	34.444451086508906	36.0	36.0	36.0	32.0	36.0
34	34.42750850848457	36.0	36.0	36.0	32.0	36.0
35	34.393886378188526	36.0	36.0	36.0	32.0	36.0
36	34.36054852825136	36.0	36.0	36.0	32.0	36.0
37	34.34028624640997	36.0	36.0	36.0	32.0	36.0
38	33.91002128117453	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	4.0
21	17.0
22	56.0
23	170.0
24	477.0
25	1124.0
26	2557.0
27	4879.0
28	8963.0
29	13918.0
30	21447.0
31	31003.0
32	44211.0
33	70418.0
34	171592.0
35	381939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.119163674190034	18.39313636792384	11.945196161658835	26.542503796227283
2	15.643788739840964	20.95890156194788	37.19104437164742	26.206265326563745
3	18.22981011666122	24.974029527961765	28.738485981258748	28.057674374118264
4	12.047010000318819	16.38668076559295	36.369916150355486	35.196393083732744
5	13.888442393962622	37.23758828975912	33.334573187012886	15.539396129265374
6	33.52803490015622	36.249827305865225	16.693810642209638	13.528327151768918
7	29.54083225399356	30.837102719936237	20.971605061273287	18.650459964796916
8	27.767113333448467	33.240298049447304	19.52292958854138	19.469659028562848
9	27.66607549701426	14.098665550750919	18.518223302712077	39.71703564952275
10	16.015499942213964	26.57307542605579	31.33384787261035	26.077576759119896
11	37.504682854508694	21.093303717322975	22.370759857906915	19.031253570261416
12	25.097571926916036	23.981572256362433	28.52261888631177	22.398236930409762
13	29.906147255155922	19.528411543954036	25.07894125070572	25.486499950184317
14	23.72146901211247	20.093971927978764	25.05426566663744	31.130293393271323
15	25.14459774329218	27.691298098509787	22.383890071176364	24.780214087021672
16	25.361660409841946	25.726309748690852	24.356051850612	24.555977990855204
17	23.829203297652164	25.90591117168674	25.544981388935383	24.719904141725717
18	24.86602955984367	24.256288042424192	27.24163033457407	23.63605206315806
19	25.662147406008145	24.75391151176044	25.783564344335247	23.800376737896165
20	25.944966510710994	23.956199570125587	25.628405718551818	24.4704282006116
21	26.96060979627406	24.108101214703975	24.452958117687068	24.4783308713349
22	25.751815276216895	24.758428115593176	25.121084835104107	24.368671773085822
23	23.73611308528289	24.382652498681548	26.041576721924287	25.839657694111274
24	24.69187462970491	25.100627276567593	25.724582811931274	24.482915281796227
25	24.676199357579527	24.49566804555925	25.817306031791574	25.01082656506965
26	24.020893277965083	25.634117893987334	26.292080799385744	24.052908028661836
27	25.14223999936236	24.935405837319685	25.290092550649128	24.632261612668824
28	24.05038404416707	24.892730658972496	26.144892650954198	24.91199264590623
29	24.14788955043851	24.907210359495096	26.077010752173948	24.867889337892446
30	24.105114655316704	25.007107008972103	26.4668999359705	24.420878399740694
31	24.96698893963426	24.82551296663824	25.051077475696687	25.15642061803081
32	24.425660686151826	25.12520291506925	25.272656746079193	25.176479652699733
33	23.832790012460514	24.635018557928102	26.199888944682233	25.332302484929155
34	24.64285619399079	24.933247252177935	26.269630621511254	24.154265932320023
35	25.53887068963227	24.76825837099384	25.93154954050198	23.761321398871914
36	24.068583300787218	25.459165916113385	25.72909941576401	24.74315136733539
37	25.16744644503426	25.35714380600921	25.175284081096947	24.300125667859582
38	24.310752970995434	25.148981505835717	25.78821378945718	24.752051733711667
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	300.0
1	227.0
2	154.0
3	154.0
4	421.5
5	689.0
6	689.0
7	817.5
8	946.0
9	885.0
10	824.0
11	824.0
12	1062.5
13	1301.0
14	1653.0
15	2005.0
16	2005.0
17	3158.0
18	4311.0
19	4311.0
20	4905.5
21	5500.0
22	5527.0
23	5554.0
24	5554.0
25	6468.5
26	7383.0
27	7383.0
28	9652.0
29	11921.0
30	15574.0
31	19227.0
32	19227.0
33	24315.5
34	29404.0
35	29404.0
36	32281.5
37	35159.0
38	39651.5
39	44144.0
40	44144.0
41	45829.0
42	47514.0
43	52781.5
44	58049.0
45	58049.0
46	60840.5
47	63632.0
48	63632.0
49	66363.0
50	69094.0
51	68570.5
52	68047.0
53	68047.0
54	64246.0
55	60445.0
56	60445.0
57	59047.0
58	57649.0
59	51478.5
60	45308.0
61	45308.0
62	43205.5
63	41103.0
64	33987.5
65	26872.0
66	26872.0
67	22589.0
68	18306.0
69	18306.0
70	14824.0
71	11342.0
72	8896.0
73	6450.0
74	6450.0
75	4810.5
76	3171.0
77	3171.0
78	3063.0
79	2955.0
80	2297.5
81	1640.0
82	1640.0
83	1624.5
84	1609.0
85	1609.0
86	997.0
87	385.0
88	343.0
89	301.0
90	301.0
91	178.0
92	55.0
93	34.0
94	13.0
95	13.0
96	8.0
97	3.0
98	3.0
99	8.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007571953484294174
2	0.0
3	0.0
4	2.656825783962868E-4
5	1.328412891981434E-4
6	2.656825783962868E-4
7	3.9852386759443024E-4
8	0.002258301916368438
9	0.0043837625435387325
10	1.328412891981434E-4
11	0.004250921254340589
12	0.0
13	3.9852386759443024E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.656825783962868E-4
22	0.0
23	1.328412891981434E-4
24	0.0
25	0.0
26	0.0
27	1.328412891981434E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	752778.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.076238404317635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66591773329031	46.61183390052669
2	11.998008155491975	13.696023477196146
3	2.9520458358657873	5.054750157250464
4	1.129852758727882	2.579509816757142
5	0.5615341807913607	1.602512938751045
6	0.33159548188653376	1.1355733666770234
7	0.21686361967603285	0.8664431760496762
8	0.13240539445193233	0.6045761487804964
9	0.1119892742034178	0.5752733861844694
>10	0.6969326362012985	7.734152995683734
>50	0.09569531245916592	3.8643443687037182
>100	0.09826864436888032	11.405103471446687
>500	0.007487134808776008	2.98588717756933
>1k	0.0014038377766455014	1.2840156184234137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3048	0.4049002494759411	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2138	0.2840146763056306	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1297	0.17229515208999202	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1069	0.1420073381528153	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1053	0.13988187752564502	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1010	0.13416970209012483	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	993	0.1319114001737564	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	977	0.12978593954658613	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	917	0.1218154621946975	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	916	0.12168262090549936	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	882	0.11716601707276249	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	870	0.11557192160238476	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	854	0.11344646097521448	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	838	0.11132100034804418	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	815	0.10826565069648687	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	805	0.10693723780450545	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	775	0.10295199912856114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.656825783962868E-4
2	0.0	0.0	0.0	0.0	2.656825783962868E-4
3	0.0	0.0	0.0	0.0	2.656825783962868E-4
4	0.0	0.0	0.0	0.0	2.656825783962868E-4
5	0.0	0.0	0.0	0.0	2.656825783962868E-4
6	1.328412891981434E-4	0.0	0.0	0.0	3.9852386759443024E-4
7	1.328412891981434E-4	0.0	0.0	0.0	3.9852386759443024E-4
8	1.328412891981434E-4	0.0	0.0	0.0	3.9852386759443024E-4
9	1.328412891981434E-4	0.0	0.0	0.0	3.9852386759443024E-4
10	1.328412891981434E-4	0.0	0.0	0.0	3.9852386759443024E-4
11	1.328412891981434E-4	0.0	0.0	0.0	3.9852386759443024E-4
12	1.328412891981434E-4	0.0	0.0	0.0	5.313651567925736E-4
13	1.328412891981434E-4	0.0	0.0	0.0	6.642064459907171E-4
14	1.328412891981434E-4	0.0	0.0	0.0	6.642064459907171E-4
15	1.328412891981434E-4	0.0	0.0	0.0	0.0011955716027832906
16	2.656825783962868E-4	0.0	0.0	1.328412891981434E-4	0.0011955716027832906
17	2.656825783962868E-4	0.0	0.0	1.328412891981434E-4	0.0011955716027832906
18	2.656825783962868E-4	0.0	0.0	1.328412891981434E-4	0.0011955716027832906
19	2.656825783962868E-4	0.0	0.0	1.328412891981434E-4	0.0011955716027832906
20	2.656825783962868E-4	0.0	0.0	2.656825783962868E-4	0.0011955716027832906
21	2.656825783962868E-4	0.0	0.0	2.656825783962868E-4	0.0014612541811795776
22	2.656825783962868E-4	0.0	0.0	5.313651567925736E-4	0.0014612541811795776
23	2.656825783962868E-4	0.0	0.0	0.001594095470377721	0.0014612541811795776
24	2.656825783962868E-4	0.0	0.0	0.0027896670731610116	0.0014612541811795776
25	2.656825783962868E-4	0.0	0.0	0.003985238675944303	0.0014612541811795776
26	2.656825783962868E-4	0.0	0.0	0.00504796898952945	0.0014612541811795776
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTAA	35	0.0020646604	22.859468	9
TACGCCT	95	1.718945E-9	20.212582	5
CATTCCG	40	0.00447943	20.002033	9
ATAAGAT	65	9.423855E-6	19.693003	3
GATATAC	90	2.0347215E-8	19.556246	1
ACCGTCC	50	7.1878626E-4	19.201952	8
CACCGTC	60	1.1366326E-4	18.668564	7
GTACGCC	80	2.7926417E-6	18.000633	4
TGCGTAG	80	2.7944134E-6	17.99944	13
GAGTAGT	45	0.0088457195	17.778404	1
TCTAGCG	260	0.0	17.230232	28
TAACGAA	140	4.0017767E-11	17.142323	13
TAGTACG	85	4.9808023E-6	16.940647	2
ACGCCTG	95	7.675553E-7	16.843819	6
TGAACGG	60	0.002439199	16.001627	5
TAGAACC	190	0.0	16.000565	4
CTAGTAC	120	3.1035597E-8	16.000565	1
CTAGCGG	270	0.0	15.999503	29
ACGTTTT	190	0.0	15.999502	29
AACCCGT	70	3.6951562E-4	15.999502	29
>>END_MODULE