FastQCFastQC Report
Thu 2 Feb 2017
SRR4063041_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063041_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001771
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45040.44960375175564077No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA29980.29926999284267564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28620.28569403586248754No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25550.2550483094439747No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25070.25025679521567307No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC24500.2445668720695648No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20700.20663405109550984No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20680.2064344046693306No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20320.20284076899810435No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19890.1985483708352508No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19580.19545385122947262No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18940.18906516559173703No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17910.17878337464350635No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17570.17538938539845933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16730.16700423549893137No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16620.1659061801549456No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16540.16510759445022863No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16440.16410936231933246No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15540.1551252731412668No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15150.1512321678307717No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT14570.14544242147157385No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14340.14314648757051263No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14090.14065090724327217No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14080.14055108403018254No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14000.1397524983254656No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG13340.1331641662615508No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG13090.13066858593431033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13030.13006964665577264No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC12820.12797335918089064No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12690.12667565741072562No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT12610.12587707170600868No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT12530.12507848600129173No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC12440.12418007708348514No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT12120.12098573426461735No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11690.11669333610176379No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC11360.11339917006980638No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG11250.11230111472582058No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT11210.11190182187346209No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT10950.10930641833313204No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG10840.10820836298914623No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10840.10820836298914623No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10580.10561295944881614No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC10520.10501402017027844No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC10310.10291773269539646No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC10160.10142038449905218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC400.00448138420.00131213
ACCGTAT400.00448656619.9973188
CTAGCGG5400.019.2595629
CGATTAA601.13829585E-418.66602724
TAGCGGC5600.018.57171830
TCTAGCG5600.018.56986428
CGGTCCA5900.017.89947710
AGCGGCG6200.017.80672831
GTATTAC450.00880553117.7931561
GAACCGG450.00885634517.7753936
ATCGTTT3450.017.62346329
CGCCGGT5750.017.5280827
CTTATAT550.001365250417.4530753
GCCGGTC6050.017.4522048
TAATACC550.001366164217.4513324
CCGGTCC6000.017.3327399
CTATTCC4650.017.2011364
CATCGTT3450.017.15797428
TACACAT753.236744E-517.0643775
GTCCTAT4600.017.0582221
Produced by FastQC (version 0.11.5)