##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063041_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001771
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227449187488958	32.0	32.0	32.0	32.0	32.0
2	30.89649929974016	32.0	32.0	32.0	32.0	32.0
3	30.953509334967773	32.0	32.0	32.0	32.0	32.0
4	31.004937256119412	32.0	32.0	32.0	32.0	32.0
5	30.945858883916582	32.0	32.0	32.0	32.0	32.0
6	34.58394683016378	36.0	36.0	36.0	32.0	36.0
7	34.488005741831216	36.0	36.0	36.0	32.0	36.0
8	34.47811825257469	36.0	36.0	36.0	32.0	36.0
9	34.59234895000953	36.0	36.0	36.0	32.0	36.0
10	34.35806686358459	36.0	36.0	36.0	32.0	36.0
11	34.593852287598665	36.0	36.0	36.0	32.0	36.0
12	34.41290075276685	36.0	36.0	36.0	32.0	36.0
13	34.5112905045165	36.0	36.0	36.0	32.0	36.0
14	34.39607055903994	36.0	36.0	36.0	32.0	36.0
15	34.35630897680208	36.0	36.0	36.0	32.0	36.0
16	34.358523055668414	36.0	36.0	36.0	32.0	36.0
17	34.26286047410037	36.0	36.0	36.0	32.0	36.0
18	34.304929969024855	36.0	36.0	36.0	32.0	36.0
19	34.29014415470202	36.0	36.0	36.0	32.0	36.0
20	34.263544263110035	36.0	36.0	36.0	32.0	36.0
21	34.22233324781811	36.0	36.0	36.0	32.0	36.0
22	34.21374146386749	36.0	36.0	36.0	32.0	36.0
23	34.15200380126795	36.0	36.0	36.0	32.0	36.0
24	34.1328976382826	36.0	36.0	36.0	32.0	36.0
25	34.09823901869789	36.0	36.0	36.0	32.0	36.0
26	34.05544081431784	36.0	36.0	36.0	32.0	36.0
27	34.03670399722092	36.0	36.0	36.0	32.0	36.0
28	34.03406966262749	36.0	36.0	36.0	32.0	36.0
29	33.99506773504124	36.0	36.0	36.0	32.0	36.0
30	33.95246718062312	36.0	36.0	36.0	32.0	36.0
31	33.98869601934973	36.0	36.0	36.0	32.0	36.0
32	33.92346354605993	36.0	36.0	36.0	32.0	36.0
33	33.86170791528203	36.0	36.0	36.0	32.0	36.0
34	33.87542262652842	36.0	36.0	36.0	32.0	36.0
35	33.811159436637716	36.0	36.0	36.0	32.0	36.0
36	33.760085887892544	36.0	36.0	36.0	32.0	36.0
37	33.7615932184102	36.0	36.0	36.0	32.0	36.0
38	33.136190806082425	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	2.0
9	2.0
10	9.0
11	12.0
12	3.0
13	2.0
14	152.0
15	315.0
16	406.0
17	471.0
18	627.0
19	822.0
20	1101.0
21	1506.0
22	2116.0
23	3058.0
24	4410.0
25	6579.0
26	9499.0
27	13609.0
28	18994.0
29	26224.0
30	35554.0
31	47368.0
32	64646.0
33	97405.0
34	217307.0
35	449568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.470139095708	17.532275441082703	11.456457386823585	24.54112807638571
2	16.903743625289895	20.14638112226859	36.647316978652086	26.30255827378943
3	18.9320000638947	25.027754260977908	27.80258134594189	28.237664329185503
4	12.056261823118659	15.188296422742315	36.048794852981544	36.706646901157484
5	14.398628825607501	37.25369820787028	32.44889789961558	15.898775066906643
6	33.713660534514446	35.67039906405569	17.131893680079937	13.484046721349932
7	30.792566364967644	30.785079623985922	20.402367407321634	18.019986603724803
8	29.764365894196743	30.567348629668633	19.392472286414478	20.275813189720143
9	28.159641912740184	14.259203986555754	18.85917583629726	38.72197826440679
10	17.01478591764128	25.21422211219409	29.648799517650865	28.122192452513765
11	38.14972353464377	21.75366582381086	21.17108708434115	18.925523557204222
12	25.6409078975346	24.513473016532185	26.961406268873354	22.884212817059865
13	29.034080925224835	18.990211817702594	25.872390217408668	26.103317039663903
14	24.695958964693105	20.197589235619574	24.105247815157426	31.001203984529894
15	25.811675830896252	26.382796875608438	23.07508744042918	24.73043985306613
16	26.518597351564622	25.700245994600895	23.215440953308946	24.565715700525534
17	23.838911298374533	25.760912975757815	24.88477974359998	25.515395982267663
18	25.864142994763977	23.58016002555617	26.60597076014635	23.9497262195335
19	25.365728992171295	25.37082034705071	25.72471942641395	23.53873123436405
20	25.923348843524103	23.189315837864765	25.39684758117893	25.490487737432204
21	28.306081518847325	23.472732046396803	23.860570006998056	24.360616427757815
22	26.35150244584207	23.79834281721074	25.09553758610362	24.754617150843565
23	24.004963700692443	23.61132186983364	25.988147816850226	26.395566612623693
24	24.464117058599186	25.629231902781303	25.0697371652666	24.836913873352913
25	25.065446202323116	24.270207450982742	25.01133202740454	25.653014319289607
26	24.123977107986246	25.596562204214973	26.143702100298334	24.13575858750045
27	25.963980512738598	24.49694513217794	24.795443654660172	24.74363070042329
28	24.254455100592285	23.975534008108077	26.587948492438485	25.182062398861156
29	23.693944358420662	25.002171393315702	26.591431632059148	24.712452616204484
30	24.12811707628726	24.90696188631781	26.921855970611137	24.043065066783797
31	24.57861971752247	25.204251139054495	24.572829607031192	25.64429953639184
32	24.295945484567206	24.85489638593668	24.60751643708982	26.241641692406294
33	23.646965687091754	24.404483209925594	25.53896253642583	26.40958856655682
34	25.082538234146018	24.68779232811232	25.892995306776672	24.33667413096499
35	26.48354094969384	24.14010684829479	25.699401062492573	23.676951139518792
36	24.009400808293798	25.67111547738854	24.915736170348715	25.403747543968947
37	26.05425911598073	25.2643821598822	24.92914368432875	23.752215039808323
38	24.001026252292345	24.779700170609452	25.63983536038127	25.57943821671693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	86.0
1	131.0
2	176.0
3	176.0
4	510.0
5	844.0
6	844.0
7	1088.0
8	1332.0
9	1351.5
10	1371.0
11	1371.0
12	1772.5
13	2174.0
14	2739.5
15	3305.0
16	3305.0
17	4698.0
18	6091.0
19	6091.0
20	7221.5
21	8352.0
22	8343.5
23	8335.0
24	8335.0
25	9039.0
26	9743.0
27	9743.0
28	12815.0
29	15887.0
30	19182.0
31	22477.0
32	22477.0
33	28435.0
34	34393.0
35	34393.0
36	37375.5
37	40358.0
38	46743.5
39	53129.0
40	53129.0
41	55411.0
42	57693.0
43	68061.0
44	78429.0
45	78429.0
46	81720.5
47	85012.0
48	85012.0
49	87689.0
50	90366.0
51	90068.5
52	89771.0
53	89771.0
54	84317.0
55	78863.0
56	78863.0
57	77523.5
58	76184.0
59	68130.0
60	60076.0
61	60076.0
62	60331.5
63	60587.0
64	50617.5
65	40648.0
66	40648.0
67	34884.0
68	29120.0
69	29120.0
70	23524.5
71	17929.0
72	13783.5
73	9638.0
74	9638.0
75	7206.5
76	4775.0
77	4775.0
78	4809.0
79	4843.0
80	3887.5
81	2932.0
82	2932.0
83	3139.5
84	3347.0
85	3347.0
86	2239.5
87	1132.0
88	977.0
89	822.0
90	822.0
91	611.5
92	401.0
93	353.5
94	306.0
95	306.0
96	289.0
97	272.0
98	272.0
99	422.0
100	572.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1162940432494053
2	0.054802943986200434
3	0.01227825521002305
4	0.0025954035403300754
5	1.9964642617923656E-4
6	1.9964642617923656E-4
7	0.0
8	5.989392785377098E-4
9	3.992928523584731E-4
10	6.98762491627328E-4
11	0.001796817835613129
12	0.002795049966509312
13	0.015472598028890835
14	0.009682851669692974
15	0.023158985436791443
16	0.01227825521002305
17	0.021062697961909457
18	0.005590099933018624
19	0.007287094555542136
20	0.00638868563773557
21	0.00638868563773557
22	0.0070874481293628985
23	0.009882498095872211
24	0.015272951602711598
25	0.018467294421579385
26	0.01986481940483404
27	0.009083912391155264
28	0.005390453506839387
29	0.010980553439858012
30	0.0030945196057781668
31	0.006288862424645952
32	0.007287094555542136
33	0.007786210620990226
34	0.011379846292216484
35	0.016371006946697398
36	0.015472598028890835
37	0.009583028456603356
38	0.0067879784900940435
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1001771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.61536957488893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88587303970498	29.66631536406124
2	14.043611261843564	11.126857006080103
3	4.836165818458487	5.7475948867103455
4	2.1181232639564027	3.356409436272117
5	1.1078538272990293	2.194401940170311
6	0.627682073859069	1.4919514398875853
7	0.4299702070873779	1.1923400061970604
8	0.28276570878935153	0.8961494445436464
9	0.20489154844225893	0.7305168972880212
>10	1.074584939442099	8.169821183080812
>50	0.16211934325699454	4.5485895897221535
>100	0.18665130534374708	15.997051636703501
>500	0.028326485362235237	7.499463334887491
>1k	0.011381177154469515	7.382537834395657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4504	0.44960375175564077	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2998	0.29926999284267564	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2862	0.28569403586248754	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2555	0.2550483094439747	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2507	0.25025679521567307	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2450	0.2445668720695648	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2070	0.20663405109550984	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2068	0.2064344046693306	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2032	0.20284076899810435	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1989	0.1985483708352508	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1958	0.19545385122947262	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1894	0.18906516559173703	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1791	0.17878337464350635	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1757	0.17538938539845933	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1673	0.16700423549893137	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1662	0.1659061801549456	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1654	0.16510759445022863	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1644	0.16410936231933246	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1554	0.1551252731412668	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1515	0.1512321678307717	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1457	0.14544242147157385	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1434	0.14314648757051263	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1409	0.14065090724327217	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1408	0.14055108403018254	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1400	0.1397524983254656	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1334	0.1331641662615508	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1309	0.13066858593431033	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1303	0.13006964665577264	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1282	0.12797335918089064	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1269	0.12667565741072562	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1261	0.12587707170600868	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1253	0.12507848600129173	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1244	0.12418007708348514	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1212	0.12098573426461735	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1169	0.11669333610176379	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1136	0.11339917006980638	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1125	0.11230111472582058	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1121	0.11190182187346209	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1095	0.10930641833313204	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1084	0.10820836298914623	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1084	0.10820836298914623	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1058	0.10561295944881614	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1052	0.10501402017027844	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1031	0.10291773269539646	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1016	0.10142038449905218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.982321308961828E-5	0.0
8	0.0	0.0	0.0	9.982321308961828E-5	0.0
9	0.0	0.0	0.0	1.9964642617923656E-4	9.982321308961828E-5
10	0.0	0.0	0.0	1.9964642617923656E-4	9.982321308961828E-5
11	0.0	0.0	0.0	1.9964642617923656E-4	9.982321308961828E-5
12	0.0	0.0	0.0	1.9964642617923656E-4	9.982321308961828E-5
13	0.0	0.0	0.0	1.9964642617923656E-4	9.982321308961828E-5
14	0.0	0.0	0.0	1.9964642617923656E-4	1.9964642617923656E-4
15	0.0	0.0	0.0	1.9964642617923656E-4	1.9964642617923656E-4
16	0.0	0.0	0.0	1.9964642617923656E-4	1.9964642617923656E-4
17	0.0	0.0	0.0	2.994696392688549E-4	1.9964642617923656E-4
18	0.0	0.0	0.0	2.994696392688549E-4	1.9964642617923656E-4
19	0.0	0.0	0.0	2.994696392688549E-4	1.9964642617923656E-4
20	0.0	0.0	0.0	3.992928523584731E-4	1.9964642617923656E-4
21	0.0	0.0	0.0	7.985857047169462E-4	1.9964642617923656E-4
22	0.0	0.0	0.0	9.98232130896183E-4	1.9964642617923656E-4
23	0.0	0.0	0.0	0.001796817835613129	2.994696392688549E-4
24	0.0	0.0	0.0	0.0025954035403300754	2.994696392688549E-4
25	0.0	0.0	0.0	0.0033939892450470218	2.994696392688549E-4
26	0.0	0.0	0.0	0.004492044589032823	2.994696392688549E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGAC	40	0.004481384	20.001312	13
ACCGTAT	40	0.004486566	19.997318	8
CTAGCGG	540	0.0	19.25956	29
CGATTAA	60	1.13829585E-4	18.666027	24
TAGCGGC	560	0.0	18.571718	30
TCTAGCG	560	0.0	18.569864	28
CGGTCCA	590	0.0	17.899477	10
AGCGGCG	620	0.0	17.806728	31
GTATTAC	45	0.008805531	17.793156	1
GAACCGG	45	0.008856345	17.775393	6
ATCGTTT	345	0.0	17.623463	29
CGCCGGT	575	0.0	17.528082	7
CTTATAT	55	0.0013652504	17.453075	3
GCCGGTC	605	0.0	17.452204	8
TAATACC	55	0.0013661642	17.451332	4
CCGGTCC	600	0.0	17.332739	9
CTATTCC	465	0.0	17.201136	4
CATCGTT	345	0.0	17.157974	28
TACACAT	75	3.236744E-5	17.064377	5
GTCCTAT	460	0.0	17.058222	1
>>END_MODULE