FastQCFastQC Report
Thu 2 Feb 2017
SRR4063041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001771
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51530.5143890170508031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34070.3400976869963295No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA30260.30206504280918495No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC29430.2937797161227466No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26310.2626348736387857No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24610.2456649274135506No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21070.21032750997982572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20970.20932927784892957No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20790.2075324600133164No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20650.20613493503006175No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20620.20583546539079292No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19740.1970510226389065No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19520.1948549119509349No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18120.18087966211838832No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17980.1794821371351337No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17850.17818443536496864No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16030.16001661058265812No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15810.1578204998946865No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.15602368205907338No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15230.15203075353548864No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14630.14604136075011154No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14460.14434436612758805No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG14300.14274719471815417No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13570.13546010016261203No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC13400.1337631055400885No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13350.1332639894746404No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13330.13306434304846118No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13260.13236558055683387No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT13140.13116770199975844No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13060.13036911629504147No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC13010.12987000022959339No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG12970.1294707073772349No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT12910.12887176809869721No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12630.1260767181321879No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12620.12597689491909828No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC12490.12467919314893325No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT12310.12288237531332012No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC11920.11898927000282498No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT11800.11779139144574957No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG11560.11539563433159875No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC11450.11429757898761295No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT11130.11110323616874515No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC10870.10850783262841507No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG10860.10840800941532545No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG10760.10740977728442927No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG10520.10501402017027844No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG10350.10331702554775492No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC10350.10331702554775492No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT10260.10241861662994836No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG10100.10082144522051448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCC350.00206590722.8579983
TCTAGCG4400.018.18159128
CATCGTT3950.017.82256128
ATACTTC450.00884759517.7784426
CGGCATC4000.017.59978125
GTAGTAA550.001364137617.4551981
CGTTTAT4350.017.28714231
GCTCTAA652.0994633E-417.2314151
CTAGCGG4650.017.20408629
ATCGTTT4100.017.17051729
TAACCTA1051.1881093E-716.7625315
TAGCGGC4700.016.68064330
AGCGGCG4900.016.32632631
GCATCGT4350.016.18370627
GTCCTAG1407.421477E-1016.0005991
CGATGTT805.6203036E-515.999820
AAGACGG5850.015.8638435
CGCAAGA5850.015.8638432
CAAGACG5800.015.7247284
TAACGAA2050.015.60956213
Produced by FastQC (version 0.11.5)