##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001771
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.141134051594626	32.0	32.0	32.0	32.0	32.0
2	31.312982707624798	32.0	32.0	32.0	32.0	32.0
3	31.387734322514827	32.0	32.0	32.0	32.0	32.0
4	31.483913988326673	32.0	32.0	32.0	32.0	32.0
5	31.404160232228723	32.0	32.0	32.0	32.0	32.0
6	34.92372009171757	36.0	36.0	36.0	36.0	36.0
7	34.94476382326899	36.0	36.0	36.0	36.0	36.0
8	34.89492908059826	36.0	36.0	36.0	36.0	36.0
9	34.997988562256246	36.0	36.0	36.0	36.0	36.0
10	34.85336169643561	36.0	36.0	36.0	32.0	36.0
11	35.02123439388842	36.0	36.0	36.0	36.0	36.0
12	34.89704433448363	36.0	36.0	36.0	32.0	36.0
13	34.96793079456283	36.0	36.0	36.0	36.0	36.0
14	34.89674785954075	36.0	36.0	36.0	32.0	36.0
15	34.87522397833437	36.0	36.0	36.0	32.0	36.0
16	34.87205758601517	36.0	36.0	36.0	32.0	36.0
17	34.82641841299059	36.0	36.0	36.0	32.0	36.0
18	34.828737306230664	36.0	36.0	36.0	32.0	36.0
19	34.81580121604638	36.0	36.0	36.0	32.0	36.0
20	34.801624323323395	36.0	36.0	36.0	32.0	36.0
21	34.78043884280938	36.0	36.0	36.0	32.0	36.0
22	34.76845806077437	36.0	36.0	36.0	32.0	36.0
23	34.698277350811715	36.0	36.0	36.0	32.0	36.0
24	34.66379841301056	36.0	36.0	36.0	32.0	36.0
25	34.63037859950028	36.0	36.0	36.0	32.0	36.0
26	34.56766167118034	36.0	36.0	36.0	32.0	36.0
27	34.5513894892146	36.0	36.0	36.0	32.0	36.0
28	34.517347777086776	36.0	36.0	36.0	32.0	36.0
29	34.46506636746322	36.0	36.0	36.0	32.0	36.0
30	34.43001444441894	36.0	36.0	36.0	32.0	36.0
31	34.435589570869986	36.0	36.0	36.0	32.0	36.0
32	34.38858082336183	36.0	36.0	36.0	32.0	36.0
33	34.35075281676151	36.0	36.0	36.0	32.0	36.0
34	34.332007015575414	36.0	36.0	36.0	32.0	36.0
35	34.268256916999995	36.0	36.0	36.0	32.0	36.0
36	34.234986838309354	36.0	36.0	36.0	32.0	36.0
37	34.21962005288634	36.0	36.0	36.0	32.0	36.0
38	33.7614963898935	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	24.0
22	84.0
23	218.0
24	688.0
25	1708.0
26	3770.0
27	6990.0
28	12525.0
29	19938.0
30	29847.0
31	42851.0
32	60838.0
33	95212.0
34	228624.0
35	498446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.648245540474164	18.84291926730799	12.179786677528462	25.32904851468938
2	15.853822879680088	21.105821589964176	35.93535848013168	27.104997050224057
3	17.946716365317023	25.556739015204073	28.318048735689093	28.178495883789807
4	11.542769298341934	15.969234455014625	36.08003833215209	36.40795791449135
5	13.74127793804965	37.64736416542719	32.36750950817054	16.243848388352617
6	33.681050858928835	36.2169597642575	16.6594960325264	13.442493344287268
7	30.327641385313537	31.1044695068509	20.448587793955873	18.11930131387969
8	29.593259761715807	30.817523421629257	19.261488702214635	20.3277281144403
9	27.972367941621286	14.021073437585788	18.759552176972953	39.24700644381997
10	16.699808337326306	25.51928605654049	29.786276153969016	27.994629452164194
11	38.02007734649994	21.702142102730686	21.263599028077536	19.014181522691835
12	25.242146642589258	24.636717646483373	27.06482232929947	23.0563133816279
13	29.081754376507956	19.004980190043813	25.76711796831405	26.146147465134177
14	24.523369113300344	20.176567299312918	24.14284302500272	31.15722056238402
15	25.688006540416918	26.395453651583047	22.99717200837317	24.91936779962686
16	26.479331583097913	25.76539525040678	23.116483823632173	24.63878934286313
17	23.83748381616158	25.872479838206537	24.849291904037948	25.44074444159394
18	25.893442712955355	23.354039995168556	26.680948041019352	24.071569250856733
19	25.43475504880856	25.262560006228966	25.66854101386445	23.634143931098027
20	25.80310270510925	23.042891039968218	25.550849445631783	25.603156809290745
21	28.08199486908173	23.636862752927318	24.053225790351078	24.22791658763988
22	26.158499455962946	23.876738173433022	25.174740708945166	24.790021661658866
23	23.958968666491643	23.313811240293443	26.075220783991547	26.651999309223367
24	24.37043995084705	25.463903427030726	25.35779135151646	24.80786527060576
25	24.999326193311646	23.909057059946832	25.377556347708207	25.71406039903331
26	24.19609710422263	25.352950630334938	26.30356898646295	24.147383278979483
27	26.06598926499023	24.238621877897998	24.854532332304156	24.840856524807613
28	24.214016975935618	23.738459188776677	26.785363121911093	25.26216071337661
29	23.803843393350377	24.624789497799398	26.905250800831727	24.6661163080185
30	24.107505607568996	24.630978537010954	27.163992569160015	24.097523286260035
31	24.72630970551154	24.75765419442168	24.75216391770175	25.763872182365034
32	24.431631580470984	24.55341590044032	24.88612666966802	26.12882584942068
33	23.702822301703684	24.13296052690685	25.852115902736255	26.312101268653215
34	25.09505665466459	24.28638880542559	26.12882584942068	24.489728690489144
35	26.510449993062284	23.876913985331978	25.78223965357352	23.830396368032215
36	24.049907613616288	25.330439791129912	25.20126855339194	25.418384041861863
37	26.236335449918197	24.89630863740316	25.07399395670268	23.793361955975968
38	24.18766364767996	24.280698882279484	25.818475479925052	25.71316199011551
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	67.5
2	102.0
3	102.0
4	384.5
5	667.0
6	667.0
7	963.0
8	1259.0
9	1349.5
10	1440.0
11	1440.0
12	1831.0
13	2222.0
14	2861.0
15	3500.0
16	3500.0
17	5071.0
18	6642.0
19	6642.0
20	7623.5
21	8605.0
22	8534.5
23	8464.0
24	8464.0
25	9084.5
26	9705.0
27	9705.0
28	12576.0
29	15447.0
30	18868.0
31	22289.0
32	22289.0
33	28706.5
34	35124.0
35	35124.0
36	37531.5
37	39939.0
38	46210.5
39	52482.0
40	52482.0
41	54568.0
42	56654.0
43	66947.5
44	77241.0
45	77241.0
46	81887.5
47	86534.0
48	86534.0
49	89085.5
50	91637.0
51	90690.5
52	89744.0
53	89744.0
54	84493.0
55	79242.0
56	79242.0
57	78126.0
58	77010.0
59	68825.5
60	60641.0
61	60641.0
62	60822.0
63	61003.0
64	50868.5
65	40734.0
66	40734.0
67	35123.5
68	29513.0
69	29513.0
70	23770.0
71	18027.0
72	13865.0
73	9703.0
74	9703.0
75	7075.0
76	4447.0
77	4447.0
78	4686.0
79	4925.0
80	3846.0
81	2767.0
82	2767.0
83	2709.5
84	2652.0
85	2652.0
86	1688.0
87	724.0
88	610.0
89	496.0
90	496.0
91	292.5
92	89.0
93	67.0
94	45.0
95	45.0
96	28.5
97	12.0
98	12.0
99	11.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009483205243513737
2	0.0
3	0.0
4	9.982321308961828E-5
5	9.982321308961828E-5
6	0.0
7	4.991160654480915E-4
8	0.0025954035403300754
9	0.003593635671226258
10	0.0010980553439858011
11	0.004492044589032823
12	1.9964642617923656E-4
13	1.9964642617923656E-4
14	0.0
15	0.0
16	9.982321308961828E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	9.982321308961828E-5
22	9.982321308961828E-5
23	0.0
24	0.0
25	0.0
26	1.9964642617923656E-4
27	1.9964642617923656E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1001771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.12615744735416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38632727194515	26.874261833339418
2	15.40868685051813	11.441306681386235
3	5.314224329911602	5.918901875485749
4	2.3803436732864993	3.534920559729917
5	1.237344707338756	2.2968927210654506
6	0.6973209805223303	1.5533309108529252
7	0.4534865881139433	1.1785350129407193
8	0.3016754313684254	0.8960039650386116
9	0.21665768806334978	0.7239300695297702
>10	1.1884947715357148	8.524074784500673
>50	0.1685523477007915	4.491000984216319
>100	0.20096576325362583	16.259251996410004
>500	0.03241383278233233	8.048346284926646
>1k	0.013235648386119035	7.742481849008755
>5k	2.701152731861028E-4	0.5167604715688173
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5153	0.5143890170508031	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3407	0.3400976869963295	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3026	0.30206504280918495	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2943	0.2937797161227466	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2631	0.2626348736387857	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2461	0.2456649274135506	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2107	0.21032750997982572	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2097	0.20932927784892957	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2079	0.2075324600133164	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2065	0.20613493503006175	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2062	0.20583546539079292	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1974	0.1970510226389065	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1952	0.1948549119509349	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1812	0.18087966211838832	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1798	0.1794821371351337	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1785	0.17818443536496864	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1603	0.16001661058265812	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1581	0.1578204998946865	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1563	0.15602368205907338	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1523	0.15203075353548864	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1463	0.14604136075011154	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1446	0.14434436612758805	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1430	0.14274719471815417	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1357	0.13546010016261203	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1340	0.1337631055400885	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1335	0.1332639894746404	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1333	0.13306434304846118	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1326	0.13236558055683387	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1314	0.13116770199975844	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1306	0.13036911629504147	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1301	0.12987000022959339	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1297	0.1294707073772349	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1291	0.12887176809869721	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1263	0.1260767181321879	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1262	0.12597689491909828	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1249	0.12467919314893325	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1231	0.12288237531332012	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1192	0.11898927000282498	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	1180	0.11779139144574957	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1156	0.11539563433159875	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1145	0.11429757898761295	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1113	0.11110323616874515	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC	1087	0.10850783262841507	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1086	0.10840800941532545	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1076	0.10740977728442927	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1052	0.10501402017027844	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG	1035	0.10331702554775492	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1035	0.10331702554775492	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1026	0.10241861662994836	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG	1010	0.10082144522051448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	9.982321308961828E-5
10	0.0	0.0	0.0	0.0	9.982321308961828E-5
11	0.0	0.0	0.0	0.0	9.982321308961828E-5
12	0.0	0.0	0.0	0.0	9.982321308961828E-5
13	0.0	0.0	0.0	0.0	9.982321308961828E-5
14	0.0	0.0	0.0	0.0	1.9964642617923656E-4
15	0.0	0.0	0.0	9.982321308961828E-5	1.9964642617923656E-4
16	0.0	0.0	0.0	9.982321308961828E-5	1.9964642617923656E-4
17	0.0	0.0	0.0	1.9964642617923656E-4	1.9964642617923656E-4
18	0.0	0.0	0.0	1.9964642617923656E-4	1.9964642617923656E-4
19	0.0	0.0	0.0	1.9964642617923656E-4	1.9964642617923656E-4
20	0.0	0.0	0.0	1.9964642617923656E-4	1.9964642617923656E-4
21	0.0	0.0	0.0	5.989392785377098E-4	1.9964642617923656E-4
22	0.0	0.0	0.0	7.985857047169462E-4	1.9964642617923656E-4
23	0.0	0.0	0.0	0.0016969946225235109	1.9964642617923656E-4
24	0.0	0.0	0.0	0.002395757114150839	1.9964642617923656E-4
25	0.0	0.0	0.0	0.003194342818867785	1.9964642617923656E-4
26	0.0	0.0	0.0	0.003893105310495113	1.9964642617923656E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCC	35	0.002065907	22.857998	3
TCTAGCG	440	0.0	18.181591	28
CATCGTT	395	0.0	17.822561	28
ATACTTC	45	0.008847595	17.778442	6
CGGCATC	400	0.0	17.599781	25
GTAGTAA	55	0.0013641376	17.455198	1
CGTTTAT	435	0.0	17.287142	31
GCTCTAA	65	2.0994633E-4	17.231415	1
CTAGCGG	465	0.0	17.204086	29
ATCGTTT	410	0.0	17.170517	29
TAACCTA	105	1.1881093E-7	16.762531	5
TAGCGGC	470	0.0	16.680643	30
AGCGGCG	490	0.0	16.326326	31
GCATCGT	435	0.0	16.183706	27
GTCCTAG	140	7.421477E-10	16.000599	1
CGATGTT	80	5.6203036E-5	15.9998	20
AAGACGG	585	0.0	15.863843	5
CGCAAGA	585	0.0	15.863843	2
CAAGACG	580	0.0	15.724728	4
TAACGAA	205	0.0	15.609562	13
>>END_MODULE