FastQCFastQC Report
Thu 2 Feb 2017
SRR4063040_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063040_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143274
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26200.22916641155138665No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21350.1867443849855765No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20210.17677302204021084No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15940.13942414504309553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15380.13452593166642468No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15320.1340011230903528No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15310.13391365499434082No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15050.13163948449802934No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14830.1297151863857658No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14610.12779088827350224No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14350.12551671777719076No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13680.11965635534438814No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12500.10933512001497453No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12310.10767322619074692No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12090.10574892807848338No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11920.10426197044627972No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11800.10321235329413597No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11760.10286248091008804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3750.020.90578328
CTAGCGG3800.020.21055429
AGTTAGG400.004474357220.0070292
TAGCGGC3900.019.69233530
TACCGTC2600.019.6906137
ATACCGT2600.019.6888896
AAGACGG3850.019.5290785
CAAGACG4250.019.1975064
ACCGTCG2850.019.0860758
CGTCTTA1351.8189894E-1218.9621615
GACGGAC4050.018.5663017
CGTCGTA2700.018.36959310
AGACGGA4050.018.1696856
CCGTCGT3100.017.5476449
GCGCAAG4200.017.1639021
CATCGTT3000.017.06594328
CGGCATC3150.016.76119425
TCGCGTA1354.4383341E-1016.591899
GCGTAAC1354.4383341E-1016.5918911
AGCGGCG4650.016.51615131
Produced by FastQC (version 0.11.5)