FastQCFastQC Report
Thu 2 Feb 2017
SRR4063040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143274
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27190.23782575305657264No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22240.1945290455306427No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19950.17449885154389935No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17860.1562180194773956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17590.15385638088507217No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16700.14607172034000598No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16150.1412609750593471No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15750.13776225121886793No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15010.13128961211398144No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14830.1297151863857658No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14510.12691620731338243No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13720.12000622772843607No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13150.11502054625575321No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13080.11440826958366937No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12950.11327118433551361No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12720.1112594181272381No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12690.11099701383920214No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12510.10942258811098651No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12080.1056614599824714No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT11570.10120058708586042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3900.020.10083428
AAGACGG3750.019.2050675
ACCGAGT507.183143E-419.2050678
CTAGCGG4200.018.6650629
TACGCTC450.0088434917.7801344
CAAGACG4050.017.3850214
GCGCAAG4050.017.3819791
TAGCGGC4500.017.06519730
CGCAAGA4050.016.9891622
GCGTAAC2150.016.37354911
GAACTAG908.559258E-616.0042216
TACTATA1001.3131357E-616.0014232
GCGGCGC4800.015.665318532
GTCGCGT2350.015.6637068
CGCGTAA2250.015.64651910
CGAGGTT2050.015.6118264
AGCGGCG5100.015.37122431
AGACGGA4600.015.3083856
TCGCGTA2300.015.3063769
GACGGAC4500.015.2935927
Produced by FastQC (version 0.11.5)