##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143274
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1883887851906	32.0	32.0	32.0	32.0	32.0
2	31.238808019774787	32.0	32.0	32.0	32.0	32.0
3	31.33778254381714	32.0	32.0	32.0	32.0	32.0
4	31.442564074753733	32.0	32.0	32.0	32.0	32.0
5	31.35244569543259	32.0	32.0	32.0	32.0	32.0
6	34.87887418064261	36.0	36.0	36.0	36.0	36.0
7	34.918895207972895	36.0	36.0	36.0	36.0	36.0
8	34.83803095320982	36.0	36.0	36.0	32.0	36.0
9	34.96715485526654	36.0	36.0	36.0	32.0	36.0
10	34.804902411845276	36.0	36.0	36.0	32.0	36.0
11	34.98778770443481	36.0	36.0	36.0	36.0	36.0
12	34.875747196210185	36.0	36.0	36.0	32.0	36.0
13	34.93588938434706	36.0	36.0	36.0	36.0	36.0
14	34.87193008850022	36.0	36.0	36.0	32.0	36.0
15	34.84283819976663	36.0	36.0	36.0	32.0	36.0
16	34.858517730657745	36.0	36.0	36.0	32.0	36.0
17	34.81431572833809	36.0	36.0	36.0	32.0	36.0
18	34.816335366675006	36.0	36.0	36.0	32.0	36.0
19	34.82267680363587	36.0	36.0	36.0	32.0	36.0
20	34.8108983498269	36.0	36.0	36.0	32.0	36.0
21	34.79273385032809	36.0	36.0	36.0	32.0	36.0
22	34.7773062275535	36.0	36.0	36.0	32.0	36.0
23	34.71416125968053	36.0	36.0	36.0	32.0	36.0
24	34.69422465655652	36.0	36.0	36.0	32.0	36.0
25	34.67161852714222	36.0	36.0	36.0	32.0	36.0
26	34.60352373971594	36.0	36.0	36.0	32.0	36.0
27	34.593171890552924	36.0	36.0	36.0	32.0	36.0
28	34.56215307966419	36.0	36.0	36.0	32.0	36.0
29	34.5238035676487	36.0	36.0	36.0	32.0	36.0
30	34.50302989484585	36.0	36.0	36.0	32.0	36.0
31	34.50531193747081	36.0	36.0	36.0	32.0	36.0
32	34.475131945622834	36.0	36.0	36.0	32.0	36.0
33	34.44388659236544	36.0	36.0	36.0	32.0	36.0
34	34.42837412553771	36.0	36.0	36.0	32.0	36.0
35	34.398181888156294	36.0	36.0	36.0	32.0	36.0
36	34.363423816163056	36.0	36.0	36.0	32.0	36.0
37	34.35150278935758	36.0	36.0	36.0	32.0	36.0
38	33.898918369524715	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	33.0
22	94.0
23	264.0
24	755.0
25	1849.0
26	3946.0
27	7512.0
28	13532.0
29	21208.0
30	32535.0
31	47943.0
32	69320.0
33	112328.0
34	268773.0
35	563178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.77027798942068	17.959398971109362	11.790778823502654	26.47954421596731
2	16.18414849296712	19.954813942076825	36.12540486830355	27.735632696652505
3	18.634465578680175	23.551222191705577	27.922002949424197	29.892309280190048
4	12.162208260333726	15.341244551816674	35.313849472259854	37.18269771558974
5	14.620204780306384	36.41104407167486	32.79213906727521	16.17661208074355
6	35.768184851737075	34.866213056723986	15.85168196922345	13.513920122315493
7	31.350889423224103	29.790014423552115	19.92698131410893	18.932114839114856
8	29.00455544203882	32.40206849257554	18.590542034624157	20.00283403076148
9	27.041833409871195	14.33541078965208	18.124078812132343	40.49867698834438
10	16.079877192752274	26.094965690069145	30.550664549903566	27.274492567275015
11	38.632654025202456	20.979652246020457	21.155296171697184	19.232397557079903
12	25.178763703030523	23.38618470368741	27.670040909007415	23.765010684274653
13	29.566218333186097	18.667839908473145	25.175220923123216	26.590720835217546
14	23.80409245727621	19.390977141087788	24.318667266114684	32.48626313552132
15	25.586517317808326	26.66596109069217	21.847606085680248	25.899915505819255
16	26.71809207591531	25.46939753724829	22.77721701009557	25.035293376740835
17	24.533488909920106	25.732239165764287	24.55876718966757	25.175504734648037
18	25.878223418008282	24.059849170015237	25.65596698604184	24.40596042593464
19	25.75506833882341	24.859395035660743	24.91283804232406	24.472698583191782
20	25.9360398294722	23.898033192393076	24.704489037623524	25.4614379405112
21	27.181521823746387	23.99986704837777	23.99033301757323	24.828278110302612
22	25.86145216409379	23.905838762920144	24.794078054847795	25.438631018138274
23	24.336269639884787	23.576608561559663	25.378890256307983	26.708231542247564
24	24.986923519646208	24.899805296018275	24.749797511357734	25.363473672977783
25	25.21132328964586	24.112197272390123	24.849904484672063	25.826574953291953
26	24.988497945374423	25.0533118045193	25.213465888317238	24.744724361789036
27	25.802935956678763	24.3601484509824	24.444730173808004	25.392185418530833
28	24.87391473959873	24.004219460951617	25.608909150387397	25.512956649062257
29	24.714547868664905	24.532439292767965	25.57208508196635	25.18092775660078
30	24.7867965159708	24.679910502644162	25.65378028364154	24.879512697743497
31	25.212153866877056	24.611772855850827	24.35076805735108	25.825305219921034
32	25.012026863201648	24.46911239125529	24.39345248820493	26.125408257338133
33	24.60661223818612	24.031072166427293	25.15801111544564	26.204304479940944
34	25.601037021746322	24.317442712770518	25.2132034840292	24.868316781453963
35	26.345303050712253	24.140757158826318	25.093634596780824	24.420305193680605
36	24.904878445586974	25.032756801956484	24.57783523459818	25.484529517858363
37	26.278827297743145	24.688657312245358	24.553169231522805	24.479346158488692
38	24.996654342401158	24.07412752684617	25.24690078397723	25.68231734677544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	48.0
2	72.0
3	72.0
4	236.5
5	401.0
6	401.0
7	514.0
8	627.0
9	678.0
10	729.0
11	729.0
12	959.0
13	1189.0
14	1693.5
15	2198.0
16	2198.0
17	3185.5
18	4173.0
19	4173.0
20	5045.5
21	5918.0
22	6421.5
23	6925.0
24	6925.0
25	8149.0
26	9373.0
27	9373.0
28	11979.5
29	14586.0
30	17781.0
31	20976.0
32	20976.0
33	27761.0
34	34546.0
35	34546.0
36	38787.0
37	43028.0
38	50207.0
39	57386.0
40	57386.0
41	61913.5
42	66441.0
43	76985.5
44	87530.0
45	87530.0
46	92758.0
47	97986.0
48	97986.0
49	104092.0
50	110198.0
51	111424.0
52	112650.0
53	112650.0
54	107328.0
55	102006.0
56	102006.0
57	99084.5
58	96163.0
59	86654.5
60	77146.0
61	77146.0
62	73464.0
63	69782.0
64	57759.5
65	45737.0
66	45737.0
67	38261.0
68	30785.0
69	30785.0
70	24395.5
71	18006.0
72	14023.5
73	10041.0
74	10041.0
75	7479.0
76	4917.0
77	4917.0
78	4995.0
79	5073.0
80	3932.0
81	2791.0
82	2791.0
83	2640.5
84	2490.0
85	2490.0
86	1612.0
87	734.0
88	608.0
89	482.0
90	482.0
91	292.0
92	102.0
93	62.5
94	23.0
95	23.0
96	19.0
97	15.0
98	15.0
99	20.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008834277697209942
2	8.746809601197963E-5
3	0.0
4	2.624042880359389E-4
5	0.0
6	8.746809601197963E-5
7	4.373404800598982E-4
8	0.0022741704963114705
9	0.004285936704587002
10	0.001661893824227613
11	0.004548340992622941
12	4.373404800598982E-4
13	2.624042880359389E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	8.746809601197963E-5
22	8.746809601197963E-5
23	8.746809601197963E-5
24	0.0
25	1.7493619202395925E-4
26	0.0
27	8.746809601197963E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.746809601197963E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1143274.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.395584696979626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9557870142295	41.36934625820746
2	12.739823797726604	13.350210336367619
3	3.7674043238991892	5.921860570218817
4	1.526718477726867	3.1997322923272744
5	0.8069767818180281	2.1141010160121265
6	0.48212855738795557	1.5156844598071937
7	0.31428080512844153	1.1526848580619544
8	0.21420314094532295	0.8978639051007786
9	0.15552713943923735	0.7334041866450768
>10	0.8388967525722433	8.029074916224046
>50	0.0870632518545291	3.238562859262082
>100	0.093748396634094	10.4239005843698
>500	0.01291346316463042	4.640303790212492
>1k	0.004528097473311966	3.413269967183269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2719	0.23782575305657264	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2224	0.1945290455306427	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1995	0.17449885154389935	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1786	0.1562180194773956	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1759	0.15385638088507217	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1670	0.14607172034000598	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1615	0.1412609750593471	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1575	0.13776225121886793	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1501	0.13128961211398144	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1483	0.1297151863857658	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1451	0.12691620731338243	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1372	0.12000622772843607	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1315	0.11502054625575321	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1308	0.11440826958366937	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1295	0.11327118433551361	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1272	0.1112594181272381	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1269	0.11099701383920214	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1251	0.10942258811098651	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1208	0.1056614599824714	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1157	0.10120058708586042	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.746809601197963E-5
2	0.0	0.0	0.0	0.0	8.746809601197963E-5
3	0.0	0.0	0.0	0.0	8.746809601197963E-5
4	0.0	0.0	0.0	0.0	8.746809601197963E-5
5	0.0	0.0	0.0	0.0	8.746809601197963E-5
6	0.0	0.0	0.0	0.0	2.624042880359389E-4
7	0.0	0.0	0.0	0.0	2.624042880359389E-4
8	0.0	0.0	0.0	0.0	2.624042880359389E-4
9	0.0	0.0	0.0	0.0	2.624042880359389E-4
10	0.0	0.0	0.0	0.0	2.624042880359389E-4
11	0.0	0.0	0.0	0.0	2.624042880359389E-4
12	0.0	0.0	0.0	0.0	5.248085760718778E-4
13	0.0	0.0	0.0	0.0	6.122766720838575E-4
14	0.0	0.0	0.0	0.0	6.122766720838575E-4
15	0.0	0.0	0.0	8.746809601197963E-5	6.122766720838575E-4
16	0.0	0.0	0.0	8.746809601197963E-5	6.99744768095837E-4
17	0.0	0.0	0.0	8.746809601197963E-5	6.99744768095837E-4
18	0.0	0.0	0.0	8.746809601197963E-5	6.99744768095837E-4
19	0.0	0.0	0.0	1.7493619202395925E-4	6.99744768095837E-4
20	0.0	0.0	0.0	1.7493619202395925E-4	6.99744768095837E-4
21	0.0	0.0	0.0	2.624042880359389E-4	6.99744768095837E-4
22	0.0	0.0	0.0	3.498723840479185E-4	6.99744768095837E-4
23	0.0	0.0	0.0	6.122766720838575E-4	6.99744768095837E-4
24	0.0	0.0	0.0	9.621490561317759E-4	6.99744768095837E-4
25	0.0	0.0	0.0	0.0010496171521437556	6.99744768095837E-4
26	0.0	0.0	0.0	0.0014869576322036537	6.99744768095837E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	390	0.0	20.100834	28
AAGACGG	375	0.0	19.205067	5
ACCGAGT	50	7.183143E-4	19.205067	8
CTAGCGG	420	0.0	18.66506	29
TACGCTC	45	0.00884349	17.780134	4
CAAGACG	405	0.0	17.385021	4
GCGCAAG	405	0.0	17.381979	1
TAGCGGC	450	0.0	17.065197	30
CGCAAGA	405	0.0	16.989162	2
GCGTAAC	215	0.0	16.373549	11
GAACTAG	90	8.559258E-6	16.004221	6
TACTATA	100	1.3131357E-6	16.001423	2
GCGGCGC	480	0.0	15.6653185	32
GTCGCGT	235	0.0	15.663706	8
CGCGTAA	225	0.0	15.646519	10
CGAGGTT	205	0.0	15.611826	4
AGCGGCG	510	0.0	15.371224	31
AGACGGA	460	0.0	15.308385	6
TCGCGTA	230	0.0	15.306376	9
GACGGAC	450	0.0	15.293592	7
>>END_MODULE