FastQCFastQC Report
Thu 2 Feb 2017
SRR4063039_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063039_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832126
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29990.3604021506358412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19260.23145533248570527No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13270.15947104164513548No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13000.15622634072243868No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12680.15238076925850172No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12430.14937641655230097No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11760.14132475129968297No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11440.13747917983574603No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11320.13603709053676966No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11070.13303273783056893No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11060.13291256372232088No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11010.13231169318108077No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10930.1313503003150965No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10850.13038890744911227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10690.1284661217171438No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9960.11969341181503762No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9540.11464609926862038No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9290.11164174656241962No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9010.1082768715314748No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8940.1074356527737386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.10551286704177013No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT8570.10298921076856149No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8500.10214799201082529No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8470.10178746968608118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATA400.004458050220.0188751
CAATAGT751.5112892E-619.1950264
GTATAGA601.12950045E-418.6842841
AATAGTG1006.827577E-817.595445
CATCGTT1750.016.45485728
GTATTAG2050.016.4057121
ACCTAGT600.00242582216.01511
CTTATAC600.00244341115.9977763
GGCGTTA1357.887138E-915.40897131
CTATACT1052.1889591E-615.2341484
TCCTACA1807.2759576E-1215.1135522
TATTAGC1704.7293724E-1115.0612562
ATACCAT859.448053E-515.0549226
GTCCTAA1501.9426807E-914.9474281
CTATTGA756.1972666E-414.9474281
TCTTATA756.234137E-414.9357462
ATATATC756.2483683E-414.9312583
ATAGTAC756.2483683E-414.9312583
TAGCACA756.2540686E-414.9294644
CGATTTG2800.014.85507828
Produced by FastQC (version 0.11.5)