FastQCFastQC Report
Thu 2 Feb 2017
SRR4063039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832126
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37480.4504125577136155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25180.30259840456853887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15250.18326551507824537No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14310.17196914890293058No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13240.15911051932039139No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12980.1559859925059426No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12430.14937641655230097No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12400.1490158942275569No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12190.14649223795434826No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11770.141444925407931No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11650.14000283610895464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.13747917983574603No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11330.1361572646450177No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11200.1345950012377933No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11110.13351343426356105No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10800.12978803690787213No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10670.12822577350064773No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10170.12221706808824624No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10090.12125567522226202No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9550.11476627337686841No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9340.11224261710365978No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8690.10443130006753784No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT8670.1041909518510418No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG8450.10154712146958514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACC502.796734E-522.3996623
TCTAGCG2700.020.14784428
ATAACCG400.004481288620.0009025
TAGACGA400.004482847619.99974
GTATATA659.425012E-619.6931951
CTAGCGG2850.018.52603729
CCTAATA802.7947026E-617.999732
CGTTTAT2300.016.69540231
TACCGTC2700.016.593347
CGACCCG2150.016.3728315
TAGCGGC3250.016.2459130
CTACTAA600.002440411416.0007219
TAGACTC600.002440411416.0007215
ACCGTCG2600.016.0007218
TAGGACG908.57632E-615.9997614
GCGAGGA1203.106834E-815.9997620
CGGTCCA3450.015.76882610
TAACGAA1451.2187229E-915.44804313
CCGTCGT2700.015.4081029
CGTCGTA2700.015.40810210
Produced by FastQC (version 0.11.5)