FastQCFastQC Report
Thu 2 Feb 2017
SRR4063038_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063038_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439444
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29150.6633382182940262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19130.4353228170142271No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11340.25805335833462284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10450.2378004933506886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9780.2225539545425583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.21914055033178287No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9630.21914055033178287No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7830.17817969980247766No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4560.10376748800757321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG654.7293724E-1027.0721128
CGTCGTA509.639625E-725.6041910
TGTAGTA453.522638E-421.3416842
CTAGCGG851.0542863E-820.702229
GTCGTAG659.38954E-619.69777311
ACCGTCG659.426549E-619.6888078
TACCGTC659.426549E-619.6888077
TAGCGGC902.033994E-819.55207830
AGCGGCG902.033994E-819.55207831
ATACCGT802.7959886E-617.9947516
GTATTAG1352.3646862E-1117.792841
ACCGTCT450.00885087917.7746188
GCGGCGC1006.799564E-817.5968732
GTCTTAC550.001355112417.4693361
GACAGTC550.001364452917.4514437
GTCCTAC753.202978E-517.0811271
GTCCTAA753.202978E-517.0811271
CCGTCGT753.228123E-517.0655759
ACGTTTT753.2312782E-517.06363329
GTATAAG805.5676966E-516.0135571
Produced by FastQC (version 0.11.5)