##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063038_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439444
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.026208117530334	32.0	32.0	32.0	32.0	32.0
2	30.669159210274803	32.0	32.0	32.0	32.0	32.0
3	30.758440210811845	32.0	32.0	32.0	32.0	32.0
4	30.795161613311365	32.0	32.0	32.0	32.0	32.0
5	30.762188128635277	32.0	32.0	32.0	32.0	32.0
6	34.35867368765986	36.0	36.0	36.0	32.0	36.0
7	34.2483843220069	36.0	36.0	36.0	32.0	36.0
8	34.218828792747196	36.0	36.0	36.0	32.0	36.0
9	34.31902130874469	36.0	36.0	36.0	32.0	36.0
10	34.08062688306132	36.0	36.0	36.0	32.0	36.0
11	34.38312959102866	36.0	36.0	36.0	32.0	36.0
12	34.208078845086064	36.0	36.0	36.0	32.0	36.0
13	34.276331000081925	36.0	36.0	36.0	32.0	36.0
14	34.1914601177852	36.0	36.0	36.0	32.0	36.0
15	34.154015073592994	36.0	36.0	36.0	32.0	36.0
16	34.16433720792638	36.0	36.0	36.0	32.0	36.0
17	34.10436597154586	36.0	36.0	36.0	32.0	36.0
18	34.1129313405121	36.0	36.0	36.0	32.0	36.0
19	34.10427267183077	36.0	36.0	36.0	32.0	36.0
20	34.04649966776199	36.0	36.0	36.0	32.0	36.0
21	34.00655601168749	36.0	36.0	36.0	32.0	36.0
22	33.99472515269295	36.0	36.0	36.0	32.0	36.0
23	33.96467354202128	36.0	36.0	36.0	32.0	36.0
24	33.95406468173419	36.0	36.0	36.0	32.0	36.0
25	33.931390575363416	36.0	36.0	36.0	32.0	36.0
26	33.89389546790945	36.0	36.0	36.0	32.0	36.0
27	33.89148560453664	36.0	36.0	36.0	32.0	36.0
28	33.86647217848008	36.0	36.0	36.0	32.0	36.0
29	33.84459908429743	36.0	36.0	36.0	32.0	36.0
30	33.78125995576228	36.0	36.0	36.0	32.0	36.0
31	33.81664102820837	36.0	36.0	36.0	32.0	36.0
32	33.76497119086846	36.0	36.0	36.0	32.0	36.0
33	33.69914482846506	36.0	36.0	36.0	27.0	36.0
34	33.672071981867994	36.0	36.0	36.0	27.0	36.0
35	33.61030529487261	36.0	36.0	36.0	27.0	36.0
36	33.573356332092374	36.0	36.0	36.0	27.0	36.0
37	33.55926124830468	36.0	36.0	36.0	27.0	36.0
38	32.936198924094995	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	0.0
9	1.0
10	6.0
11	3.0
12	3.0
13	3.0
14	170.0
15	287.0
16	330.0
17	356.0
18	451.0
19	570.0
20	781.0
21	903.0
22	1210.0
23	1782.0
24	2409.0
25	3451.0
26	4810.0
27	6826.0
28	9350.0
29	12367.0
30	16900.0
31	22365.0
32	30009.0
33	43752.0
34	94060.0
35	186285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.834728858084816	17.79892140176759	11.98100719293329	24.385342547214307
2	15.553895930909018	21.023522902440636	38.80880440994651	24.613776756703835
3	19.023862899279674	26.030019231425744	28.623872002913163	26.322245866381422
4	12.209225587693147	16.333887080991282	36.4608697631022	34.99601756821337
5	13.641390672716764	37.784508535824976	33.62127425234729	14.952826539110964
6	31.971536760087748	36.36436041907501	18.07056189184515	13.59354092899209
7	28.495098351553327	31.81838869116429	21.483283421778427	18.203229535503954
8	27.632770650051658	33.01687139599765	20.26774864487236	19.08260930907833
9	27.661898498550435	14.966707779411163	19.17795750064855	38.19343622138985
10	15.584309192111814	27.700583922337874	32.38629898826238	24.328807897287923
11	35.5393528948602	22.570624940264068	22.63684648889253	19.253175675983197
12	24.416286627160762	25.01410925111736	29.537489645630227	21.032114476091646
13	29.70425702372499	20.79191019828481	25.658218167913915	23.84561461007629
14	23.51210186734032	20.48544020755812	26.809818045266788	29.192639879834775
15	24.661422412812005	27.448115118655796	24.723333743040666	23.167128725491533
16	23.91450797248888	26.927576208618394	25.59116585416923	23.566749964723503
17	22.5589493809177	26.65536234522942	26.821285506190822	23.964402767662055
18	23.385879991534274	25.759923899512767	28.442784677039455	22.411411431913503
19	24.19119186549298	26.157683449700965	27.48491165466578	22.166213030140277
20	24.700056438545705	24.780617911045567	27.747055182332915	22.77227046807581
21	25.75965153432314	24.986117811985107	26.172248368273966	23.081982285417784
22	24.42308217494606	25.360260716789107	26.77171805445558	23.44493905380925
23	23.179391241414155	25.586158867146423	27.072551241960376	24.161898649479046
24	23.29430882466737	26.603274599323594	26.99450591967809	23.107910656330937
25	23.85499006516252	25.63119789331373	26.93057904284157	23.58323299868218
26	22.905219936543197	26.300204390992228	27.63215994391766	23.16241572854691
27	23.71791662304678	26.327372201309963	26.962098488400144	22.992612687243117
28	23.110341406132655	26.01736842823917	27.37165978635377	23.500630379274405
29	22.831704935717553	26.5124980370469	27.465412197392286	23.19038482984326
30	23.37101101656923	26.44909644106329	27.472925091425466	22.706967450942017
31	23.270066680321342	26.51692041601238	26.55447076762022	23.658542136046062
32	22.841485534044438	26.91216196226312	26.540300538675638	23.706051965016805
33	22.652450472798442	26.501746680169774	27.140337501849093	23.70546534518269
34	23.16293529790638	26.64896001893564	27.271196200126084	22.91690848303189
35	23.738457721747167	26.54706927984377	27.017067891470152	22.69740510693891
36	22.926183111766594	27.3786845285647	26.796033420503402	22.899098939165306
37	23.52121875561847	27.332561066365653	26.41699230084729	22.729227877168587
38	22.83938536043622	27.433934439660273	26.55389475025716	23.172785449646348
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1138.0
1	758.0
2	378.0
3	378.0
4	713.5
5	1049.0
6	1049.0
7	1183.0
8	1317.0
9	1226.0
10	1135.0
11	1135.0
12	1446.5
13	1758.0
14	2091.5
15	2425.0
16	2425.0
17	3329.0
18	4233.0
19	4233.0
20	4871.0
21	5509.0
22	5322.0
23	5135.0
24	5135.0
25	5725.0
26	6315.0
27	6315.0
28	8088.5
29	9862.0
30	11770.5
31	13679.0
32	13679.0
33	15658.5
34	17638.0
35	17638.0
36	19135.5
37	20633.0
38	23368.0
39	26103.0
40	26103.0
41	28160.5
42	30218.0
43	32853.0
44	35488.0
45	35488.0
46	38254.0
47	41020.0
48	41020.0
49	40812.5
50	40605.0
51	38879.5
52	37154.0
53	37154.0
54	34988.0
55	32822.0
56	32822.0
57	31068.5
58	29315.0
59	26100.0
60	22885.0
61	22885.0
62	20909.5
63	18934.0
64	15548.5
65	12163.0
66	12163.0
67	10108.0
68	8053.0
69	8053.0
70	6505.5
71	4958.0
72	3861.0
73	2764.0
74	2764.0
75	2086.0
76	1408.0
77	1408.0
78	1313.0
79	1218.0
80	933.0
81	648.0
82	648.0
83	666.5
84	685.0
85	685.0
86	453.0
87	221.0
88	202.0
89	183.0
90	183.0
91	130.0
92	77.0
93	60.0
94	43.0
95	43.0
96	47.5
97	52.0
98	52.0
99	137.5
100	223.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12311011186863399
2	0.058027871583182386
3	0.013426056562383375
4	0.003185843930057072
5	4.551205614367245E-4
6	0.0
7	0.0
8	4.551205614367245E-4
9	4.551205614367245E-4
10	4.551205614367245E-4
11	0.002275602807183623
12	0.003640964491493796
13	0.017294581334595535
14	0.011150453755199753
15	0.024121389756146402
16	0.013198496281665015
17	0.019115063580342432
18	0.005689007017959057
19	0.007281928982987592
20	0.006371687860114144
21	0.007281928982987592
22	0.009102411228734493
23	0.013198496281665015
24	0.014108737404538461
25	0.018432382738187345
26	0.020480425264652605
27	0.009557531790171216
28	0.00591656729867742
29	0.012515815439509926
30	0.0034134042107754345
31	0.007737049544424318
32	0.007509489263705956
33	0.00887485094801613
34	0.013881177123820101
35	0.018432382738187345
36	0.01661190049244045
37	0.010240212632326303
38	0.007281928982987592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	439444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.52284399791401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.36730427067995	60.04500621330855
2	9.500831544476608	13.210496586342153
3	2.0432573031071173	4.2615917619454375
4	0.705181947344225	1.9610501806143106
5	0.3325292952075052	1.1559191157723838
6	0.19041496360304083	0.794291388566561
7	0.12802697262152282	0.6230559471563937
8	0.08470346602464465	0.47110606836111835
9	0.065468943943558	0.40964284588465444
>10	0.4764101878624885	6.729821931062813
>50	0.06510086595632007	3.164540769953313
>100	0.03813990116185675	4.728981458418085
>500	0.001315169005581267	0.8427955644425503
>1k	0.001315169005581267	1.6017001681716707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2915	0.6633382182940262	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1913	0.4353228170142271	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1134	0.25805335833462284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1045	0.2378004933506886	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	978	0.2225539545425583	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	963	0.21914055033178287	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	963	0.21914055033178287	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	783	0.17817969980247766	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	456	0.10376748800757321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.275602807183623E-4	0.0
12	0.0	0.0	0.0	4.551205614367246E-4	0.0
13	0.0	0.0	0.0	4.551205614367246E-4	0.0
14	0.0	0.0	0.0	6.826808421550868E-4	0.0
15	0.0	0.0	0.0	6.826808421550868E-4	0.0
16	0.0	0.0	0.0	6.826808421550868E-4	0.0
17	0.0	0.0	0.0	6.826808421550868E-4	0.0
18	0.0	0.0	0.0	9.102411228734492E-4	0.0
19	0.0	0.0	0.0	9.102411228734492E-4	0.0
20	0.0	0.0	0.0	0.0011378014035918114	0.0
21	0.0	0.0	0.0	0.0018204822457468983	0.0
22	0.0	0.0	0.0	0.0027307233686203474	0.0
23	0.0	0.0	0.0	0.006144127579395782	0.0
24	0.0	0.0	0.0	0.010012652351607941	0.0
25	0.0	0.0	0.0	0.013653616843101738	0.0
26	0.0	0.0	0.0	0.018887503299624072	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	65	4.7293724E-10	27.07211	28
CGTCGTA	50	9.639625E-7	25.60419	10
TGTAGTA	45	3.522638E-4	21.341684	2
CTAGCGG	85	1.0542863E-8	20.7022	29
GTCGTAG	65	9.38954E-6	19.697773	11
ACCGTCG	65	9.426549E-6	19.688807	8
TACCGTC	65	9.426549E-6	19.688807	7
TAGCGGC	90	2.033994E-8	19.552078	30
AGCGGCG	90	2.033994E-8	19.552078	31
ATACCGT	80	2.7959886E-6	17.994751	6
GTATTAG	135	2.3646862E-11	17.79284	1
ACCGTCT	45	0.008850879	17.774618	8
GCGGCGC	100	6.799564E-8	17.59687	32
GTCTTAC	55	0.0013551124	17.469336	1
GACAGTC	55	0.0013644529	17.451443	7
GTCCTAC	75	3.202978E-5	17.081127	1
GTCCTAA	75	3.202978E-5	17.081127	1
CCGTCGT	75	3.228123E-5	17.065575	9
ACGTTTT	75	3.2312782E-5	17.063633	29
GTATAAG	80	5.5676966E-5	16.013557	1
>>END_MODULE