FastQCFastQC Report
Thu 2 Feb 2017
SRR4063038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439444
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37810.8604054213961279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27060.6157781196238884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16000.36409644914937966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.2940078826881241No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12330.28058182612574073No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10630.24189657840361914No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6320.14381809741400498No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.14336297685256824No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.13426056562383376No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5180.11787622541211167No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.11173209783271588No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4840.11013917586768734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATA200.003749445832.002738
CCGATAA200.003749445832.002739
AACGAAT308.4099494E-426.66590931
AAATCGC401.5916752E-423.99931714
CCTACTC350.002064863722.8564913
TCTAGCG604.7107733E-621.33272628
ATCGCTC453.5326486E-421.33272616
GCATCGT604.7107733E-621.33272627
TTCTATA400.004479886519.999432
CCAACGA400.004479886519.9994329
ATCCCGT659.414398E-619.6917520
CGTCGTA507.182135E-419.2016410
TACCGTC507.182135E-419.201647
TAGGACA852.3883513E-718.8229924
GTCGTAG601.1354075E-418.6682611
ACCGTCG601.1354075E-418.668268
AATCCCG601.13639915E-418.66613619
GGACGGC1055.9408194E-918.28727510
TCGTTTA701.7836106E-518.28519430
ATCGTTT701.7836106E-518.28519429
Produced by FastQC (version 0.11.5)