##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063038_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439444
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05330372015547	32.0	32.0	32.0	32.0	32.0
2	31.250093299715093	32.0	32.0	32.0	32.0	32.0
3	31.348417545807884	32.0	32.0	32.0	32.0	32.0
4	31.45611044865785	32.0	32.0	32.0	32.0	32.0
5	31.363513894830742	32.0	32.0	32.0	32.0	32.0
6	34.85704663165272	36.0	36.0	36.0	32.0	36.0
7	34.88896651222909	36.0	36.0	36.0	32.0	36.0
8	34.849209455584784	36.0	36.0	36.0	32.0	36.0
9	34.94858503017449	36.0	36.0	36.0	32.0	36.0
10	34.78222936255814	36.0	36.0	36.0	32.0	36.0
11	34.980625517699636	36.0	36.0	36.0	36.0	36.0
12	34.874903286880695	36.0	36.0	36.0	32.0	36.0
13	34.92550814210684	36.0	36.0	36.0	32.0	36.0
14	34.86342514632126	36.0	36.0	36.0	32.0	36.0
15	34.83089767979538	36.0	36.0	36.0	32.0	36.0
16	34.849421086645854	36.0	36.0	36.0	32.0	36.0
17	34.81877554364151	36.0	36.0	36.0	32.0	36.0
18	34.80498311502717	36.0	36.0	36.0	32.0	36.0
19	34.78657803952267	36.0	36.0	36.0	32.0	36.0
20	34.773509252601016	36.0	36.0	36.0	32.0	36.0
21	34.757721120324774	36.0	36.0	36.0	32.0	36.0
22	34.730047514586616	36.0	36.0	36.0	32.0	36.0
23	34.695426493478124	36.0	36.0	36.0	32.0	36.0
24	34.6709319048616	36.0	36.0	36.0	32.0	36.0
25	34.653371077998564	36.0	36.0	36.0	32.0	36.0
26	34.58136872957647	36.0	36.0	36.0	32.0	36.0
27	34.5574316636477	36.0	36.0	36.0	32.0	36.0
28	34.52503163087902	36.0	36.0	36.0	32.0	36.0
29	34.481246757266	36.0	36.0	36.0	32.0	36.0
30	34.435923576155325	36.0	36.0	36.0	32.0	36.0
31	34.4107326530798	36.0	36.0	36.0	32.0	36.0
32	34.39322871628694	36.0	36.0	36.0	32.0	36.0
33	34.307169969324875	36.0	36.0	36.0	32.0	36.0
34	34.28062278697627	36.0	36.0	36.0	32.0	36.0
35	34.228930193608285	36.0	36.0	36.0	32.0	36.0
36	34.18270814938877	36.0	36.0	36.0	32.0	36.0
37	34.14758877126551	36.0	36.0	36.0	32.0	36.0
38	33.740763328205645	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	12.0
22	56.0
23	122.0
24	308.0
25	763.0
26	1625.0
27	3275.0
28	5692.0
29	9124.0
30	13514.0
31	19371.0
32	27471.0
33	42900.0
34	99102.0
35	216103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40119024590071	18.85253922918492	12.655522809253423	25.09074771566095
2	14.80507186353665	21.829630169031777	37.94840753315553	25.41689043427604
3	18.13018268539336	26.531480689234577	29.07219122345509	26.26614540191697
4	11.794931310772956	17.234772200262604	36.33190197591041	34.638394513054024
5	13.168685884890907	38.1946277568928	33.397429479069004	15.239256879147286
6	32.10739024767661	36.730049790189426	17.54262204057855	13.619937921555419
7	28.17270954367233	32.12088939862508	21.390032381901637	18.316368675800955
8	27.571085598552685	33.097045069236636	20.148144776815684	19.183724555394996
9	27.592602285217545	14.583565640268253	18.99015322017236	38.83367885434185
10	15.426714515552783	27.983861241264428	32.34692414646857	24.24250009671422
11	35.75728060333939	22.153242562371847	22.688947501371125	19.400529332917635
12	24.314926656987726	25.009898917263257	29.554525967021817	21.120648458727203
13	29.668171598656485	20.802650622149805	25.74799064272126	23.78118713647245
14	23.399796105988475	20.327732316290586	26.969761789898143	29.302709787822796
15	24.428141014554754	27.428523315826364	24.93332483774952	23.21001083186936
16	24.04885275223408	26.712451899336205	25.545065002742106	23.69363034568761
17	22.585585421578173	26.367409726836637	27.05828273909759	23.988722112487597
18	23.418228488726665	25.302655173355422	28.72106571030666	22.558050627611255
19	24.16894985481654	25.819217010586105	27.953504883443625	22.058328251153732
20	24.688014855135126	24.401516461710706	28.293479942836857	22.61698874031731
21	25.58824332565697	24.7471805281219	26.663920772612666	23.00065537360847
22	24.30639626437043	25.276030620511374	27.201418155669437	23.216154959448758
23	23.12422060603854	24.990897588771265	27.6731506175986	24.211731187591592
24	23.13104741446009	25.996941589827145	27.70523661717989	23.16677437853287
25	23.861288355285314	24.916940497537798	27.711608305040002	23.510162842136882
26	23.034439129623475	25.736274639201532	28.14091506956549	23.088371161609494
27	23.804853405667163	25.704526629104052	27.447410819125988	23.043209146102804
28	23.04798791199789	25.356359399604955	28.13623578886047	23.459416899536688
29	22.932842409954397	25.82012725170898	28.06341649903059	23.183613839306034
30	23.36725498584575	25.685184005242988	28.08867569019033	22.85888531872093
31	23.09213460645725	25.739798472615394	27.509534775762102	23.658532145165253
32	22.691400952112215	26.180127615805425	27.349104777855654	23.779366654226706
33	22.77832897934663	25.73069606138666	27.754844758376496	23.736130200890216
34	23.04889815312076	26.00831960386306	28.075022073347228	22.867760169668948
35	23.70404420130893	25.891126059293107	27.67656402180938	22.728265717588588
36	23.20204622204422	26.40768789652379	27.32225266473089	23.068013216701104
37	23.650112414778675	26.40768789652379	27.226677346829177	22.71552234186836
38	23.16750977942532	26.240718363928885	27.289318523676563	23.302453332969236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	529.0
1	378.0
2	227.0
3	227.0
4	681.5
5	1136.0
6	1136.0
7	1281.0
8	1426.0
9	1314.5
10	1203.0
11	1203.0
12	1523.5
13	1844.0
14	2248.5
15	2653.0
16	2653.0
17	3863.5
18	5074.0
19	5074.0
20	5599.0
21	6124.0
22	5756.0
23	5388.0
24	5388.0
25	5747.5
26	6107.0
27	6107.0
28	7775.5
29	9444.0
30	11278.0
31	13112.0
32	13112.0
33	15384.0
34	17656.0
35	17656.0
36	19220.0
37	20784.0
38	23442.0
39	26100.0
40	26100.0
41	27758.0
42	29416.0
43	32155.0
44	34894.0
45	34894.0
46	38251.0
47	41608.0
48	41608.0
49	41439.0
50	41270.0
51	39092.5
52	36915.0
53	36915.0
54	34963.0
55	33011.0
56	33011.0
57	31186.0
58	29361.0
59	26131.0
60	22901.0
61	22901.0
62	21042.0
63	19183.0
64	15749.0
65	12315.0
66	12315.0
67	10145.5
68	7976.0
69	7976.0
70	6392.5
71	4809.0
72	3784.5
73	2760.0
74	2760.0
75	2096.5
76	1433.0
77	1433.0
78	1345.0
79	1257.0
80	951.5
81	646.0
82	646.0
83	621.0
84	596.0
85	596.0
86	384.5
87	173.0
88	128.5
89	84.0
90	84.0
91	50.0
92	16.0
93	11.0
94	6.0
95	6.0
96	4.5
97	3.0
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00887485094801613
2	0.0
3	0.0
4	2.2756028071836226E-4
5	0.0
6	0.0
7	2.2756028071836226E-4
8	0.0020480425264652606
9	0.0025031630879019853
10	0.0011378014035918116
11	0.005233886456522333
12	4.551205614367245E-4
13	0.0
14	0.0
15	0.0
16	2.2756028071836226E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	4.551205614367245E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.2756028071836226E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	439444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.59197634243374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.32252180819034	57.671178755359875
2	10.290758666711849	13.911454326921643
3	2.1816084778501614	4.4237768586990285
4	0.7721280569083822	2.0875864542352276
5	0.3327937693884199	1.1247094293705713
6	0.19870655182246255	0.8058581129922283
7	0.13208144808628858	0.6249352280025965
8	0.09000941413737519	0.4867131352775828
9	0.07809295536852352	0.47506114726019766
>10	0.48655272212253686	6.772695537569253
>50	0.06634289493714454	3.128677702169174
>100	0.045018392904753575	5.2740240060250585
>500	0.001353936628714393	0.5422278715200152
>1k	0.0020309049430715896	2.671101434597543
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3781	0.8604054213961279	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2706	0.6157781196238884	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1600	0.36409644914937966	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1292	0.2940078826881241	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1233	0.28058182612574073	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1063	0.24189657840361914	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	632	0.14381809741400498	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	630	0.14336297685256824	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	590	0.13426056562383376	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	518	0.11787622541211167	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	491	0.11173209783271588	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	484	0.11013917586768734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.275602807183623E-4	0.0
12	0.0	0.0	0.0	2.275602807183623E-4	0.0
13	0.0	0.0	0.0	2.275602807183623E-4	0.0
14	0.0	0.0	0.0	2.275602807183623E-4	0.0
15	0.0	0.0	0.0	2.275602807183623E-4	0.0
16	0.0	0.0	0.0	2.275602807183623E-4	0.0
17	0.0	0.0	0.0	2.275602807183623E-4	0.0
18	0.0	0.0	0.0	4.551205614367246E-4	0.0
19	0.0	0.0	0.0	4.551205614367246E-4	0.0
20	0.0	0.0	0.0	6.826808421550868E-4	2.275602807183623E-4
21	0.0	0.0	0.0	0.0013653616843101737	2.275602807183623E-4
22	0.0	0.0	0.0	0.0022756028071836227	2.275602807183623E-4
23	0.0	0.0	0.0	0.005461446737240695	2.275602807183623E-4
24	0.0	0.0	0.0	0.009329971509452854	2.275602807183623E-4
25	0.0	0.0	0.0	0.012743375720228288	2.275602807183623E-4
26	0.0	0.0	0.0	0.01774970189603226	2.275602807183623E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATA	20	0.0037494458	32.00273	8
CCGATAA	20	0.0037494458	32.00273	9
AACGAAT	30	8.4099494E-4	26.665909	31
AAATCGC	40	1.5916752E-4	23.999317	14
CCTACTC	35	0.0020648637	22.856491	3
TCTAGCG	60	4.7107733E-6	21.332726	28
ATCGCTC	45	3.5326486E-4	21.332726	16
GCATCGT	60	4.7107733E-6	21.332726	27
TTCTATA	40	0.0044798865	19.99943	2
CCAACGA	40	0.0044798865	19.99943	29
ATCCCGT	65	9.414398E-6	19.69175	20
CGTCGTA	50	7.182135E-4	19.20164	10
TACCGTC	50	7.182135E-4	19.20164	7
TAGGACA	85	2.3883513E-7	18.822992	4
GTCGTAG	60	1.1354075E-4	18.66826	11
ACCGTCG	60	1.1354075E-4	18.66826	8
AATCCCG	60	1.13639915E-4	18.666136	19
GGACGGC	105	5.9408194E-9	18.287275	10
TCGTTTA	70	1.7836106E-5	18.285194	30
ATCGTTT	70	1.7836106E-5	18.285194	29
>>END_MODULE