FastQCFastQC Report
Thu 2 Feb 2017
SRR4063037_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063037_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696780
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38140.22477869847593676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23350.13761359752000848No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21410.12618017656973798No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20940.12341022407147656No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17250.10166315020214758No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17170.10119166892584779No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17090.10072018764954796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC9150.019.0805641
ACCGTCG3100.018.0631188
TAGGACG9700.017.8130554
TATAACG450.00883788117.7826922
GTAGGAC9700.017.649683
TCCTACA10650.017.281772
CCTACAG10350.017.0050183
AGGACGT9800.016.9782775
TGTAGGA10200.016.7889522
GGACGTG10300.016.7754026
TACCGTC3250.016.7371677
ATAATAC1354.4383341E-1016.5927773
TACACTA1750.016.455875
CTGTAGG10900.016.3110641
CCGTCGT3250.016.2458559
CGTCGTA3250.016.24585510
ATACCGT3250.016.2448986
TCTAGCG3550.016.22457728
ATCGTTT3750.016.21255729
TAAACGC3700.015.99923328
Produced by FastQC (version 0.11.5)