##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063037_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1696780
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246041325333866	32.0	32.0	32.0	32.0	32.0
2	30.877852756397413	32.0	32.0	32.0	32.0	32.0
3	30.920843008522024	32.0	32.0	32.0	32.0	32.0
4	30.988032037152724	32.0	32.0	32.0	32.0	32.0
5	30.90608269781586	32.0	32.0	32.0	32.0	32.0
6	34.554339395796745	36.0	36.0	36.0	32.0	36.0
7	34.49001815202914	36.0	36.0	36.0	32.0	36.0
8	34.47821461827697	36.0	36.0	36.0	32.0	36.0
9	34.60060467473686	36.0	36.0	36.0	32.0	36.0
10	34.33646495126062	36.0	36.0	36.0	32.0	36.0
11	34.581915156944326	36.0	36.0	36.0	32.0	36.0
12	34.41233159278162	36.0	36.0	36.0	32.0	36.0
13	34.4924179917255	36.0	36.0	36.0	32.0	36.0
14	34.40043199471941	36.0	36.0	36.0	32.0	36.0
15	34.342807553130044	36.0	36.0	36.0	32.0	36.0
16	34.36810370230672	36.0	36.0	36.0	32.0	36.0
17	34.290478435625126	36.0	36.0	36.0	32.0	36.0
18	34.323154445479084	36.0	36.0	36.0	32.0	36.0
19	34.304269852308494	36.0	36.0	36.0	32.0	36.0
20	34.28668713681208	36.0	36.0	36.0	32.0	36.0
21	34.2662207239595	36.0	36.0	36.0	32.0	36.0
22	34.23940817312793	36.0	36.0	36.0	32.0	36.0
23	34.18805502186495	36.0	36.0	36.0	32.0	36.0
24	34.17637878805738	36.0	36.0	36.0	32.0	36.0
25	34.15150166786501	36.0	36.0	36.0	32.0	36.0
26	34.11490941665979	36.0	36.0	36.0	32.0	36.0
27	34.103299190230906	36.0	36.0	36.0	32.0	36.0
28	34.081647001968435	36.0	36.0	36.0	32.0	36.0
29	34.05071193672721	36.0	36.0	36.0	32.0	36.0
30	34.02503211966195	36.0	36.0	36.0	32.0	36.0
31	34.03709555746767	36.0	36.0	36.0	32.0	36.0
32	33.99635721778899	36.0	36.0	36.0	32.0	36.0
33	33.964935937481584	36.0	36.0	36.0	32.0	36.0
34	33.96543924374403	36.0	36.0	36.0	32.0	36.0
35	33.92745848018011	36.0	36.0	36.0	32.0	36.0
36	33.88563691226912	36.0	36.0	36.0	32.0	36.0
37	33.88103584436403	36.0	36.0	36.0	32.0	36.0
38	33.29218284043895	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	5.0
8	7.0
9	5.0
10	16.0
11	24.0
12	6.0
13	7.0
14	191.0
15	471.0
16	620.0
17	679.0
18	844.0
19	1102.0
20	1436.0
21	2006.0
22	3105.0
23	4614.0
24	7074.0
25	10840.0
26	15918.0
27	22993.0
28	32631.0
29	43911.0
30	59531.0
31	80182.0
32	109387.0
33	165129.0
34	366668.0
35	767377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.558953637327576	17.200325476310034	11.451325773109497	25.789395113252894
2	16.470956602959177	19.325764383716734	38.37037613599107	25.832902877333012
3	19.46979123258184	23.3541418986583	28.219703556042546	28.956363312717315
4	12.569825158627909	15.140994072154923	36.2471990372114	36.041981732005766
5	14.59455190634951	36.50302897787982	33.766134265139144	15.13628485063152
6	34.295549739299865	35.401882510194326	16.92031421925215	13.382253531253665
7	30.50538697143234	30.217783223389727	20.582645117602233	18.694184687575696
8	27.77431695180664	33.64235202316874	19.35773225465576	19.22559877036886
9	27.28150865290092	14.502950307996596	18.662377738435097	39.55316330066738
10	15.975812796704316	26.431532946518814	31.602431686179287	25.990222570597577
11	37.47603485517774	21.13276380813739	22.530330310861743	18.860871025823126
12	25.210596636936877	23.38343174830129	28.852313896107034	22.553657718654797
13	29.579522848174943	19.442507478522273	25.362726750268934	25.615242923033847
14	23.222136495263175	20.155947096577215	25.045900597724508	31.5760158104351
15	24.776197533309517	27.99414275962324	22.300369850303177	24.929289856764065
16	25.332578878809848	25.871542329024688	24.496902610530537	24.298976181634927
17	23.732807044535893	26.20296898091083	25.643079756214167	24.421144218339112
18	24.640738291504437	24.86170000836932	26.946484710675122	23.551076989451126
19	25.523224390752798	24.926163017205152	25.498882204447256	24.051730387594798
20	25.648122498777013	24.614214903310604	25.35189518291713	24.385767414995254
21	26.478040615210517	24.360049627060302	24.62934420963602	24.53256554809316
22	25.578834886107316	24.777781510643678	25.288142865815455	24.355240737433554
23	23.905418284847347	24.664639483392442	25.946492027912182	25.483450203848037
24	24.651716587043595	25.14201003810708	25.70916603547819	24.49710733937113
25	24.664969433894864	24.85683650979937	25.735770447990465	24.7424236083153
26	23.947631356344335	25.899194487262996	26.247041590158183	23.906132566234486
27	24.822367308158167	25.158034891746468	25.411774829823315	24.607822970272053
28	23.958737925591763	25.318580487508747	25.863118690939352	24.85956289596014
29	24.129740027184386	25.16736788870669	25.756382613780104	24.946509470328817
30	23.90358528730863	25.488827803422147	26.218710069829175	24.388876839440048
31	24.935903707624245	25.099872337867325	25.013055014952855	24.951168939555576
32	24.35141422553698	25.406845123899817	25.0121268167386	25.229613833824594
33	23.957074704913648	25.00780963585289	25.887616687747773	25.147498971485692
34	24.792683063404294	25.22662073835421	25.892380863124554	24.088315335116945
35	25.145623513850296	25.11202488891823	26.02384441360968	23.71850718362179
36	23.90049731302427	25.702778103608747	25.580468689674195	24.816255893692784
37	25.027230868252108	25.411056439805634	25.112106301765174	24.449606390177085
38	24.20530864961557	25.49684233481565	25.543463205759537	24.754385809809243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	146.0
2	197.0
3	197.0
4	506.5
5	816.0
6	816.0
7	950.5
8	1085.0
9	1116.5
10	1148.0
11	1148.0
12	1496.0
13	1844.0
14	2651.0
15	3458.0
16	3458.0
17	5057.0
18	6656.0
19	6656.0
20	8351.5
21	10047.0
22	11345.0
23	12643.0
24	12643.0
25	15877.5
26	19112.0
27	19112.0
28	24643.5
29	30175.0
30	37823.5
31	45472.0
32	45472.0
33	56833.0
34	68194.0
35	68194.0
36	75208.0
37	82222.0
38	91921.0
39	101620.0
40	101620.0
41	105496.0
42	109372.0
43	119809.5
44	130247.0
45	130247.0
46	134170.5
47	138094.0
48	138094.0
49	145696.5
50	153299.0
51	154350.0
52	155401.0
53	155401.0
54	148655.0
55	141909.0
56	141909.0
57	136004.5
58	130100.0
59	116434.5
60	102769.0
61	102769.0
62	96235.0
63	89701.0
64	74911.5
65	60122.0
66	60122.0
67	50191.0
68	40260.0
69	40260.0
70	32062.5
71	23865.0
72	18510.0
73	13155.0
74	13155.0
75	9991.5
76	6828.0
77	6828.0
78	6481.0
79	6134.0
80	4833.5
81	3533.0
82	3533.0
83	3541.0
84	3549.0
85	3549.0
86	2360.5
87	1172.0
88	988.0
89	804.0
90	804.0
91	594.5
92	385.0
93	324.0
94	263.0
95	263.0
96	249.0
97	235.0
98	235.0
99	517.0
100	799.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11981517933969048
2	0.05327738422187909
3	0.011963837386107803
4	0.0024752767005740287
5	1.768054786124306E-4
6	1.768054786124306E-4
7	5.893515953747687E-5
8	6.482867549122455E-4
9	4.7148127629981494E-4
10	8.84027393062153E-4
11	0.002652082179186459
12	0.003123563455486274
13	0.015028465682056602
14	0.01054939355720836
15	0.024634896686665332
16	0.011197680312120605
17	0.02198281450747887
18	0.006247126910972548
19	0.00807411685663433
20	0.006482867549122456
21	0.007366894942184609
22	0.008191987175709285
23	0.010195782599983497
24	0.014439114086681833
25	0.01726800174448072
26	0.02033263004042952
27	0.009488560685533776
28	0.006659673027734886
29	0.012847864779169958
30	0.004007590848548427
31	0.006718608187272363
32	0.007484765261259562
33	0.009311755206921344
34	0.013142540576857343
35	0.016619714989568476
36	0.015499946958356416
37	0.010372588078595929
38	0.006777543346809841
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1696780.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.59464300692289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95915910340695	44.453009054876574
2	12.219275528372442	13.586525216061899
3	3.4449515377604496	5.745625527538268
4	1.47901862765007	3.2890205041917864
5	0.7432151086255266	2.0659389320693786
6	0.45677448771289225	1.5236528747440998
7	0.29511131968095117	1.1484625925475065
8	0.2172508619626837	0.9662387291009344
9	0.1656033319106142	0.82859923064948
>10	0.867167885620242	8.611598830985319
>50	0.06924071294117844	2.7001077402012728
>100	0.06969742527888695	8.332708719883275
>500	0.009697640047422733	3.6392328918174157
>1k	0.0038364290297496525	3.109279155332755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3814	0.22477869847593676	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2335	0.13761359752000848	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2141	0.12618017656973798	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2094	0.12341022407147656	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1725	0.10166315020214758	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1717	0.10119166892584779	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1709	0.10072018764954796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.893515953747687E-5
2	0.0	0.0	0.0	0.0	5.893515953747687E-5
3	0.0	0.0	0.0	0.0	5.893515953747687E-5
4	0.0	0.0	0.0	0.0	5.893515953747687E-5
5	0.0	0.0	0.0	0.0	1.1787031907495374E-4
6	0.0	0.0	0.0	0.0	1.1787031907495374E-4
7	0.0	0.0	0.0	0.0	1.1787031907495374E-4
8	0.0	0.0	0.0	0.0	1.1787031907495374E-4
9	0.0	0.0	0.0	5.893515953747687E-5	1.1787031907495374E-4
10	0.0	0.0	0.0	1.1787031907495374E-4	1.1787031907495374E-4
11	0.0	0.0	0.0	1.1787031907495374E-4	1.1787031907495374E-4
12	0.0	0.0	0.0	1.768054786124306E-4	3.536109572248612E-4
13	0.0	0.0	0.0	2.3574063814990747E-4	4.7148127629981494E-4
14	0.0	0.0	0.0	2.3574063814990747E-4	4.7148127629981494E-4
15	0.0	0.0	0.0	2.3574063814990747E-4	5.304164358372918E-4
16	0.0	0.0	0.0	2.3574063814990747E-4	6.482867549122455E-4
17	0.0	0.0	0.0	2.3574063814990747E-4	6.482867549122455E-4
18	0.0	0.0	0.0	2.3574063814990747E-4	8.250922335246762E-4
19	0.0	0.0	0.0	2.9467579768738435E-4	8.250922335246762E-4
20	0.0	0.0	0.0	2.9467579768738435E-4	0.0010608328716745837
21	0.0	0.0	0.0	6.482867549122455E-4	0.0010608328716745837
22	0.0	0.0	0.0	8.250922335246762E-4	0.0011197680312120604
23	0.0	0.0	0.0	0.0011197680312120604	0.0011197680312120604
24	0.0	0.0	0.0	0.0014144438288994448	0.0011197680312120604
25	0.0	0.0	0.0	0.0018269899456617828	0.0011197680312120604
26	0.0	0.0	0.0	0.002652082179186459	0.0011787031907495374
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAC	915	0.0	19.080564	1
ACCGTCG	310	0.0	18.063118	8
TAGGACG	970	0.0	17.813055	4
TATAACG	45	0.008837881	17.782692	2
GTAGGAC	970	0.0	17.64968	3
TCCTACA	1065	0.0	17.28177	2
CCTACAG	1035	0.0	17.005018	3
AGGACGT	980	0.0	16.978277	5
TGTAGGA	1020	0.0	16.788952	2
GGACGTG	1030	0.0	16.775402	6
TACCGTC	325	0.0	16.737167	7
ATAATAC	135	4.4383341E-10	16.592777	3
TACACTA	175	0.0	16.45587	5
CTGTAGG	1090	0.0	16.311064	1
CCGTCGT	325	0.0	16.245855	9
CGTCGTA	325	0.0	16.245855	10
ATACCGT	325	0.0	16.244898	6
TCTAGCG	355	0.0	16.224577	28
ATCGTTT	375	0.0	16.212557	29
TAAACGC	370	0.0	15.999233	28
>>END_MODULE