FastQCFastQC Report
Thu 2 Feb 2017
SRR4063037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696780
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42520.2505922983533517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26330.15517627506217657No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21240.12517827885760086No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20780.12246726151887694No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18350.10814601775127006No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18340.10808708259173257No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17910.10555287073162106No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17680.10419736206225909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC3900.017.6407641
GTAGGAC11050.017.3783823
TCTAGCG4800.017.33256728
TGTAGGA11100.017.299082
TAGGACG8100.016.5952844
CGTCTTA1354.4383341E-1016.5918615
CTGTAGG11150.016.5019991
GCGACGC805.6255165E-515.99929232
CTAGCGG5100.015.99929229
TAGCGGC5150.015.8439630
AAGACGG4300.015.6304415
AGGACGT8650.015.3550895
CGTGATT3700.015.13446628
CAAGACG4350.015.0829044
GTCCTAC9450.015.0685611
TGTTCGC859.439954E-515.05815810
CGGTCCA5750.015.02542310
GGACGTG9300.014.9701676
GCGAGAA6650.014.91663520
GTTATTC2700.014.8172173
Produced by FastQC (version 0.11.5)