##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1696780
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.202471151239408	32.0	32.0	32.0	32.0	32.0
2	31.248323884062753	32.0	32.0	32.0	32.0	32.0
3	31.350538078006576	32.0	32.0	32.0	32.0	32.0
4	31.453948066337414	32.0	32.0	32.0	32.0	32.0
5	31.370878369617746	32.0	32.0	32.0	32.0	32.0
6	34.89473001803416	36.0	36.0	36.0	36.0	36.0
7	34.91977981824397	36.0	36.0	36.0	36.0	36.0
8	34.85640035832577	36.0	36.0	36.0	32.0	36.0
9	34.98106236518582	36.0	36.0	36.0	36.0	36.0
10	34.81628673133818	36.0	36.0	36.0	32.0	36.0
11	34.996073739671615	36.0	36.0	36.0	36.0	36.0
12	34.89325840710051	36.0	36.0	36.0	32.0	36.0
13	34.947393297893655	36.0	36.0	36.0	36.0	36.0
14	34.88775327384811	36.0	36.0	36.0	32.0	36.0
15	34.85995709520385	36.0	36.0	36.0	32.0	36.0
16	34.874321361637925	36.0	36.0	36.0	32.0	36.0
17	34.839741746130905	36.0	36.0	36.0	32.0	36.0
18	34.84859321774184	36.0	36.0	36.0	32.0	36.0
19	34.83708966395172	36.0	36.0	36.0	32.0	36.0
20	34.82432195098952	36.0	36.0	36.0	32.0	36.0
21	34.82118247504096	36.0	36.0	36.0	32.0	36.0
22	34.79371751199331	36.0	36.0	36.0	32.0	36.0
23	34.74033993800021	36.0	36.0	36.0	32.0	36.0
24	34.714835158358774	36.0	36.0	36.0	32.0	36.0
25	34.69674854724832	36.0	36.0	36.0	32.0	36.0
26	34.629548910288904	36.0	36.0	36.0	32.0	36.0
27	34.6173552257806	36.0	36.0	36.0	32.0	36.0
28	34.57529909593465	36.0	36.0	36.0	32.0	36.0
29	34.54741451455109	36.0	36.0	36.0	32.0	36.0
30	34.51981871544926	36.0	36.0	36.0	32.0	36.0
31	34.521523709614684	36.0	36.0	36.0	32.0	36.0
32	34.49412357524252	36.0	36.0	36.0	32.0	36.0
33	34.462573226935724	36.0	36.0	36.0	32.0	36.0
34	34.448418180318015	36.0	36.0	36.0	32.0	36.0
35	34.42637760935419	36.0	36.0	36.0	32.0	36.0
36	34.387169815768694	36.0	36.0	36.0	32.0	36.0
37	34.37721330991643	36.0	36.0	36.0	32.0	36.0
38	33.94837397894836	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	10.0
21	27.0
22	123.0
23	343.0
24	1011.0
25	2701.0
26	5785.0
27	11213.0
28	19930.0
29	31535.0
30	48294.0
31	69762.0
32	101224.0
33	161295.0
34	391460.0
35	852065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81734089191767	18.503030152998857	12.196881553494194	26.482747401589286
2	15.556298139003372	20.367944204872877	37.489443233326206	26.586314422797546
3	18.479355013614022	24.092693218920544	28.486957649194355	28.940994118271078
4	11.990608081905824	16.10310836184816	36.20738835604894	35.69889520019708
5	14.080384068874025	37.09923331205773	33.28170610315191	15.538676515916332
6	34.42108185106233	35.870241051122214	16.456493693632105	13.252183404183343
7	30.364090916055996	30.444184081085634	20.51770005905324	18.674024943805126
8	27.801722486684703	33.8174597889002	19.060158551757997	19.320659172657102
9	27.199318689479174	14.256245837581702	18.450548997188708	40.09388647575041
10	15.723654820181027	26.905718067707785	31.456385751213194	25.914241360897993
11	37.69000185065781	21.028187758075408	22.404098794479147	18.877711596787634
12	25.123808839034663	23.48828862384921	28.77804816094589	22.609854376170237
13	29.581595235204606	19.468133132324912	25.258696187715778	25.691575444754704
14	23.177267045384227	20.134914446725237	25.034020340892948	31.65379816699759
15	24.791251664918256	28.110715590707102	22.0662077582244	25.031824986150237
16	25.469005999599243	25.927639411119884	24.289065170499416	24.314289418781456
17	23.74910123881705	26.369240561534202	25.312415280708166	24.56924291894058
18	24.708388830608566	24.898926201393227	26.870189417602752	23.522495550395455
19	25.61033251217011	24.98267306309598	25.449734202430484	23.95726022230342
20	25.761678001862354	24.600183877697756	25.191362463018187	24.446775657421703
21	26.45873875955195	24.415979971404557	24.558839303289652	24.566441965753835
22	25.56589540187885	24.9061162908568	25.166197149895687	24.36179115736866
23	23.92661140893082	24.60460308255185	25.904008543260865	25.564776965256463
24	24.67956953759474	25.194132415516453	25.620940840886856	24.505357206001957
25	24.67899473060428	24.887978929489343	25.671227661351303	24.761798678555074
26	23.98554909888141	25.850375416966255	26.116055116161203	24.048020367991136
27	24.840595033295436	25.168569389413708	25.323981496706406	24.66685408058445
28	23.988319051379673	25.27911691556949	25.87630688716274	24.856257145888094
29	24.204139605605913	25.045733683801082	25.71800704864508	25.032119661947927
30	24.008239135303338	25.350428458609837	26.164794493098693	24.47653791298813
31	24.952262520774642	24.95750774997348	25.087931258029915	25.002298471221962
32	24.47636110750952	25.315126298046884	25.080623298247268	25.127889296196326
33	23.973879937292992	24.934935583870626	25.931941677766122	25.159242801070263
34	24.848772380626833	25.20910194603897	25.86823277030611	24.07389290302809
35	25.240278645434294	25.086575749360552	25.822616956824103	23.85052864838105
36	24.0128950129068	25.69466872546824	25.58363488489963	24.708801376725326
37	25.08009288181143	25.363040582750855	25.048680441777954	24.508186093659756
38	24.34953264418487	25.241693089263194	25.590766039203665	24.81800822734827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	66.5
2	93.0
3	93.0
4	357.0
5	621.0
6	621.0
7	815.0
8	1009.0
9	1044.5
10	1080.0
11	1080.0
12	1452.0
13	1824.0
14	2581.5
15	3339.0
16	3339.0
17	5022.0
18	6705.0
19	6705.0
20	8482.5
21	10260.0
22	11338.5
23	12417.0
24	12417.0
25	15574.5
26	18732.0
27	18732.0
28	24412.5
29	30093.0
30	37255.5
31	44418.0
32	44418.0
33	56474.0
34	68530.0
35	68530.0
36	75523.0
37	82516.0
38	92137.0
39	101758.0
40	101758.0
41	105743.0
42	109728.0
43	119891.0
44	130054.0
45	130054.0
46	134222.5
47	138391.0
48	138391.0
49	146339.5
50	154288.0
51	155191.0
52	156094.0
53	156094.0
54	149221.0
55	142348.0
56	142348.0
57	136657.0
58	130966.0
59	117009.5
60	103053.0
61	103053.0
62	96981.0
63	90909.0
64	75405.5
65	59902.0
66	59902.0
67	49980.5
68	40059.0
69	40059.0
70	32000.0
71	23941.0
72	18594.5
73	13248.0
74	13248.0
75	9773.5
76	6299.0
77	6299.0
78	6240.0
79	6181.0
80	4826.0
81	3471.0
82	3471.0
83	3225.0
84	2979.0
85	2979.0
86	1864.5
87	750.0
88	626.0
89	502.0
90	502.0
91	304.5
92	107.0
93	75.0
94	43.0
95	43.0
96	31.5
97	20.0
98	20.0
99	16.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00978323648322116
2	5.893515953747687E-5
3	0.0
4	1.1787031907495374E-4
5	5.893515953747687E-5
6	1.768054786124306E-4
7	3.536109572248612E-4
8	0.0027699524982614126
9	0.0029467579768738435
10	0.0010608328716745835
11	0.00506842372022301
12	6.482867549122455E-4
13	1.1787031907495374E-4
14	5.893515953747687E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	3.536109572248612E-4
22	0.0
23	2.3574063814990747E-4
24	0.0
25	5.893515953747687E-5
26	0.0
27	5.893515953747687E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1696780.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.66819335496778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53517275100039	42.14840836366485
2	13.012003384506249	13.966614271503527
3	3.767931868125475	6.066542881407088
4	1.543368064143475	3.313191027373373
5	0.8245732559099399	2.2126678466754988
6	0.4933558376035165	1.588650989118456
7	0.33221893425364796	1.2480712999794272
8	0.23636812742415161	1.0148360292440823
9	0.17065666969599316	0.8242951631903488
>10	0.9203928090857085	8.832232087673075
>50	0.07339032346496907	2.7446891182599398
>100	0.07586069154470389	8.773667356041743
>500	0.01017345908459229	3.6987340904087667
>1k	0.004533824157263956	3.5673994754598772
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4252	0.2505922983533517	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2633	0.15517627506217657	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2124	0.12517827885760086	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2078	0.12246726151887694	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1835	0.10814601775127006	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1834	0.10808708259173257	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1791	0.10555287073162106	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1768	0.10419736206225909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9467579768738435E-4
2	0.0	0.0	0.0	0.0	2.9467579768738435E-4
3	0.0	0.0	0.0	0.0	2.9467579768738435E-4
4	0.0	0.0	0.0	0.0	2.9467579768738435E-4
5	0.0	0.0	0.0	0.0	2.9467579768738435E-4
6	0.0	0.0	0.0	0.0	2.9467579768738435E-4
7	0.0	0.0	0.0	0.0	2.9467579768738435E-4
8	0.0	0.0	0.0	0.0	2.9467579768738435E-4
9	0.0	0.0	0.0	0.0	2.9467579768738435E-4
10	0.0	0.0	0.0	0.0	3.536109572248612E-4
11	0.0	0.0	0.0	0.0	3.536109572248612E-4
12	0.0	0.0	0.0	5.893515953747687E-5	6.482867549122455E-4
13	0.0	0.0	0.0	5.893515953747687E-5	7.072219144497224E-4
14	0.0	0.0	0.0	5.893515953747687E-5	7.072219144497224E-4
15	0.0	0.0	0.0	5.893515953747687E-5	0.0010608328716745837
16	0.0	0.0	0.0	1.768054786124306E-4	0.0010608328716745837
17	0.0	0.0	0.0	2.3574063814990747E-4	0.0011197680312120604
18	0.0	0.0	0.0	2.3574063814990747E-4	0.0012376383502870141
19	0.0	0.0	0.0	2.3574063814990747E-4	0.0012376383502870141
20	0.0	0.0	0.0	2.3574063814990747E-4	0.0014733789884369217
21	0.0	0.0	0.0	6.482867549122455E-4	0.0016501844670493524
22	0.0	0.0	0.0	8.84027393062153E-4	0.0017091196265868291
23	0.0	0.0	0.0	0.001296573509824491	0.0017091196265868291
24	0.0	0.0	0.0	0.001768054786124306	0.0017091196265868291
25	0.0	0.0	0.0	0.002180600902886644	0.0017091196265868291
26	0.0	0.0	0.0	0.0028878228173363665	0.0017091196265868291
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	390	0.0	17.640764	1
GTAGGAC	1105	0.0	17.378382	3
TCTAGCG	480	0.0	17.332567	28
TGTAGGA	1110	0.0	17.29908	2
TAGGACG	810	0.0	16.595284	4
CGTCTTA	135	4.4383341E-10	16.59186	15
CTGTAGG	1115	0.0	16.501999	1
GCGACGC	80	5.6255165E-5	15.999292	32
CTAGCGG	510	0.0	15.999292	29
TAGCGGC	515	0.0	15.84396	30
AAGACGG	430	0.0	15.630441	5
AGGACGT	865	0.0	15.355089	5
CGTGATT	370	0.0	15.134466	28
CAAGACG	435	0.0	15.082904	4
GTCCTAC	945	0.0	15.068561	1
TGTTCGC	85	9.439954E-5	15.058158	10
CGGTCCA	575	0.0	15.025423	10
GGACGTG	930	0.0	14.970167	6
GCGAGAA	665	0.0	14.916635	20
GTTATTC	270	0.0	14.817217	3
>>END_MODULE