FastQCFastQC Report
Thu 2 Feb 2017
SRR4063036_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063036_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155494
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31640.2738222786098413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21280.18416365641015878No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19900.17222071252641727No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19490.16867244658994335No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17370.1503253154062245No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15120.13085312429142862No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14560.12600671228063495No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13990.12107375719821999No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13960.12081412798335604No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13760.11908326655092974No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13590.11761203433336737No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13280.1149291991131066No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13220.11440994068337872No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13180.11406376839689346No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12560.10869809795637189No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12460.10783266724015876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12060.10437094437530615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4050.020.14858428
AACCGCG1450.019.8607797
CGGTCCA4350.019.1268510
CTAGCGG4300.018.97715629
TAGCGGC4400.018.18221330
GATATAC1800.017.7897131
TCTATAC450.00885581717.7758543
TGGACGG450.00885802517.7750855
CCAACGA1453.6379788E-1217.65555429
AATCCCG1006.8192094E-817.59885819
CGCGGTC1550.017.54876510
AACGAAT1201.6589183E-917.33370831
CGCCGGT4950.017.13027
AAGACGG3950.017.010085
CCGGTCC5100.016.9415449
AGAACCG2100.016.7593655
AGCGGCG4900.016.65342131
GTCCTAC2500.016.651171
CAAGACG4250.016.1857854
GTCTTAA908.526384E-616.010741
Produced by FastQC (version 0.11.5)