##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063036_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155494
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233313197645337	32.0	32.0	32.0	32.0	32.0
2	30.85443888068653	32.0	32.0	32.0	32.0	32.0
3	30.905224951406066	32.0	32.0	32.0	32.0	32.0
4	30.962198851746525	32.0	32.0	32.0	32.0	32.0
5	30.85656870567913	32.0	32.0	32.0	32.0	32.0
6	34.526747867146	36.0	36.0	36.0	32.0	36.0
7	34.457803329138876	36.0	36.0	36.0	32.0	36.0
8	34.41732626911087	36.0	36.0	36.0	32.0	36.0
9	34.58178839526644	36.0	36.0	36.0	32.0	36.0
10	34.283065078659	36.0	36.0	36.0	32.0	36.0
11	34.5688848232877	36.0	36.0	36.0	32.0	36.0
12	34.371678260553495	36.0	36.0	36.0	32.0	36.0
13	34.47657971395784	36.0	36.0	36.0	32.0	36.0
14	34.3596470427367	36.0	36.0	36.0	32.0	36.0
15	34.297178522779	36.0	36.0	36.0	32.0	36.0
16	34.32283335093042	36.0	36.0	36.0	32.0	36.0
17	34.24049627258991	36.0	36.0	36.0	32.0	36.0
18	34.27346485572404	36.0	36.0	36.0	32.0	36.0
19	34.25155214998953	36.0	36.0	36.0	32.0	36.0
20	34.22980214522966	36.0	36.0	36.0	32.0	36.0
21	34.211848785021814	36.0	36.0	36.0	32.0	36.0
22	34.19184262315512	36.0	36.0	36.0	32.0	36.0
23	34.13924174422368	36.0	36.0	36.0	32.0	36.0
24	34.125116184073654	36.0	36.0	36.0	32.0	36.0
25	34.10391486238786	36.0	36.0	36.0	32.0	36.0
26	34.0434177936017	36.0	36.0	36.0	32.0	36.0
27	34.06571648143564	36.0	36.0	36.0	32.0	36.0
28	34.047058660624806	36.0	36.0	36.0	32.0	36.0
29	34.009229818588416	36.0	36.0	36.0	32.0	36.0
30	33.9837835592396	36.0	36.0	36.0	32.0	36.0
31	34.00255994405856	36.0	36.0	36.0	32.0	36.0
32	33.95413303747142	36.0	36.0	36.0	32.0	36.0
33	33.91875336436191	36.0	36.0	36.0	32.0	36.0
34	33.91449977239172	36.0	36.0	36.0	32.0	36.0
35	33.884374994591056	36.0	36.0	36.0	32.0	36.0
36	33.84372052126623	36.0	36.0	36.0	32.0	36.0
37	33.845040303108455	36.0	36.0	36.0	32.0	36.0
38	33.23409208528993	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	3.0
9	6.0
10	10.0
11	8.0
12	6.0
13	7.0
14	102.0
15	246.0
16	347.0
17	438.0
18	553.0
19	716.0
20	970.0
21	1412.0
22	2149.0
23	3336.0
24	5140.0
25	7784.0
26	11058.0
27	16304.0
28	22278.0
29	30338.0
30	41381.0
31	55951.0
32	76948.0
33	116890.0
34	256455.0
35	504654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.00392160600423	16.9789250501898	11.196679025299817	25.820474318506147
2	17.206833551247307	18.858765769900163	37.189948826295144	26.74445185255739
3	19.623506072650223	22.638625379107868	27.761342076472463	29.97652647176945
4	12.605077653391955	14.604549722836458	35.303235521937246	37.48713710183434
5	14.980484469792035	36.102778907649565	33.152428839713025	15.764307782845375
6	35.47000447429616	34.86541195978499	16.1716814266514	13.492902139267443
7	31.389026155935174	29.88308886336828	19.840881770811247	18.887003209885304
8	29.06558014066796	32.40417243984571	18.63543250594771	19.89481491353862
9	27.2809174297635	14.05361712660181	18.437735019545837	40.22773042408885
10	16.378446397261058	25.86305974476452	30.700434969995204	27.058058887979215
11	38.61212705523139	20.869761637296122	21.44589282592478	19.072218481547704
12	25.430345851803516	23.25936029273038	27.819252145887457	23.491041709578646
13	29.687394509928833	18.748106588466246	25.15878690096787	26.40571200063705
14	23.702504803614396	19.54664266301996	24.453599681490072	32.29725285187558
15	25.507388139158437	26.938791409502873	21.930784345974395	25.6230361053643
16	26.42833526345764	25.382865689314038	23.327326578763785	24.861472468464537
17	24.352748708094214	25.75138279363266	24.573130090801286	25.322738407471846
18	25.543044580805415	24.180521537436277	25.93398128835152	24.342452593406783
19	25.80709654693108	24.53949127883075	25.09825541995158	24.555156754286582
20	26.155784831062455	23.79016588672413	24.582513650797498	25.47153563141592
21	27.486556871379577	23.589369434397618	24.175994419359835	24.74807927486297
22	26.34934785054656	23.869881687020193	24.809548125773535	24.971222336659714
23	24.699666950554967	23.577096445856963	25.600839201512226	26.122397402075848
24	24.985869825824505	24.630213558215885	25.105922209171588	25.277994406788025
25	25.157448281831556	24.25188262788886	25.142906604345193	25.44776248593439
26	24.720175819001298	25.401742782479385	25.586892863109256	24.29118853541006
27	25.54524548704904	24.415763586022813	24.85699720528961	25.181993721638534
28	24.676009187889573	24.445965758321115	25.596442548860427	25.281582504928885
29	24.466273025171244	24.590735749537377	25.61058573646792	25.332405488823458
30	24.389009043159472	24.935372003679923	25.870930946056937	24.80468800710367
31	25.313930472698065	24.718308579222818	24.38587851632854	25.581882431750575
32	24.970551961399483	24.68286561222061	24.359261743514292	25.98732068286561
33	24.51138218559428	24.259175594295318	25.17946134765216	26.049980872458246
34	25.61775812807754	24.53487999709178	25.181612104415386	24.665749770415296
35	26.049751708867934	24.294964844537812	25.266922704724415	24.38836074186984
36	24.443596195343112	25.21004984761811	24.805746627993432	25.540607329045343
37	25.802763410260138	24.874414047623993	24.641591020820783	24.681231521295086
38	24.728691496331635	24.52651375821239	25.24270416186682	25.502090583589155
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	74.5
2	109.0
3	109.0
4	287.5
5	466.0
6	466.0
7	640.0
8	814.0
9	845.5
10	877.0
11	877.0
12	1208.5
13	1540.0
14	2096.5
15	2653.0
16	2653.0
17	3764.5
18	4876.0
19	4876.0
20	5732.5
21	6589.0
22	6968.0
23	7347.0
24	7347.0
25	8737.0
26	10127.0
27	10127.0
28	13056.0
29	15985.0
30	20089.5
31	24194.0
32	24194.0
33	30727.5
34	37261.0
35	37261.0
36	41790.0
37	46319.0
38	53418.0
39	60517.0
40	60517.0
41	64327.0
42	68137.0
43	78239.0
44	88341.0
45	88341.0
46	89598.0
47	90855.0
48	90855.0
49	97020.5
50	103186.0
51	106679.5
52	110173.0
53	110173.0
54	105916.0
55	101659.0
56	101659.0
57	98371.0
58	95083.0
59	86389.0
60	77695.0
61	77695.0
62	73886.0
63	70077.0
64	59028.0
65	47979.0
66	47979.0
67	40187.5
68	32396.0
69	32396.0
70	25869.0
71	19342.0
72	15184.0
73	11026.0
74	11026.0
75	8317.5
76	5609.0
77	5609.0
78	5320.0
79	5031.0
80	4002.5
81	2974.0
82	2974.0
83	2995.0
84	3016.0
85	3016.0
86	2058.5
87	1101.0
88	903.5
89	706.0
90	706.0
91	531.5
92	357.0
93	319.0
94	281.0
95	281.0
96	243.0
97	205.0
98	205.0
99	378.0
100	551.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11899672347930841
2	0.052272015259274385
3	0.012981460743197282
4	0.003375179793231293
5	3.461722864852608E-4
6	4.327153581065761E-4
7	8.65430716213152E-5
8	6.058015013492065E-4
9	4.327153581065761E-4
10	0.0010385168594557825
11	0.002423206005396826
12	0.002423206005396826
13	0.014885408318866217
14	0.009865910164829935
15	0.022847370908027215
16	0.011077513167528346
17	0.019385648043174608
18	0.005538756583764173
19	0.007529247231054423
20	0.005798385798628119
21	0.0061445580851133795
22	0.007269618016190477
23	0.009519737878344673
24	0.01427960681751701
25	0.016789355894535153
26	0.01860676039858277
27	0.008394677947267577
28	0.0051925842972789125
29	0.011077513167528346
30	0.0030290075067460325
31	0.0054522135121428585
32	0.00597147194187075
33	0.007961962589160999
34	0.012721831528333337
35	0.017048985109399097
36	0.014971951390487532
37	0.009519737878344673
38	0.006490730371598641
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1155494.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.65521979080636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7035708536533	40.38843382038804
2	13.914231387429501	14.653138238504738
3	4.46861924494091	7.058883855113722
4	1.8814627279263882	3.962753338686963
5	0.9322951769892472	2.4545103727138766
6	0.5168830177587844	1.6329953343734438
7	0.3181112613405425	1.1725152868671838
8	0.21250231634945982	0.8951484938749019
9	0.1562079086219318	0.7402645585395015
>10	0.7131507242496955	6.897627320938946
>50	0.07762866687354067	2.848735962335543
>100	0.09080751432061002	10.366355451302073
>500	0.009906272417838292	3.54282233437662
>1k	0.0046229271283245365	3.3858156319844874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3164	0.2738222786098413	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2128	0.18416365641015878	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1990	0.17222071252641727	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1949	0.16867244658994335	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1737	0.1503253154062245	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1512	0.13085312429142862	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1456	0.12600671228063495	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1399	0.12107375719821999	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1396	0.12081412798335604	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1376	0.11908326655092974	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1359	0.11761203433336737	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1328	0.1149291991131066	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1322	0.11440994068337872	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1318	0.11406376839689346	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1256	0.10869809795637189	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1246	0.10783266724015876	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1206	0.10437094437530615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.654307162131522E-5
7	0.0	0.0	0.0	8.654307162131522E-5	8.654307162131522E-5
8	0.0	0.0	0.0	8.654307162131522E-5	8.654307162131522E-5
9	0.0	0.0	0.0	8.654307162131522E-5	8.654307162131522E-5
10	0.0	0.0	0.0	1.7308614324263043E-4	1.7308614324263043E-4
11	0.0	0.0	0.0	1.7308614324263043E-4	1.7308614324263043E-4
12	0.0	0.0	0.0	1.7308614324263043E-4	4.3271535810657604E-4
13	0.0	0.0	0.0	1.7308614324263043E-4	4.3271535810657604E-4
14	0.0	0.0	0.0	1.7308614324263043E-4	4.3271535810657604E-4
15	0.0	0.0	0.0	1.7308614324263043E-4	4.3271535810657604E-4
16	0.0	0.0	0.0	2.5962921486394564E-4	4.3271535810657604E-4
17	0.0	0.0	0.0	3.4617228648526087E-4	4.3271535810657604E-4
18	0.0	0.0	0.0	3.4617228648526087E-4	4.3271535810657604E-4
19	0.0	0.0	0.0	4.3271535810657604E-4	4.3271535810657604E-4
20	0.0	0.0	0.0	5.192584297278913E-4	6.058015013492065E-4
21	0.0	0.0	0.0	6.923445729705217E-4	6.923445729705217E-4
22	0.0	0.0	0.0	8.654307162131521E-4	6.923445729705217E-4
23	0.0	0.0	0.0	0.0012981460743197281	6.923445729705217E-4
24	0.0	0.0	0.0	0.0019039475756689346	6.923445729705217E-4
25	0.0	0.0	0.0	0.002077033718911565	6.923445729705217E-4
26	0.0	0.0	0.0	0.002855921363503402	6.923445729705217E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	405	0.0	20.148584	28
AACCGCG	145	0.0	19.860779	7
CGGTCCA	435	0.0	19.12685	10
CTAGCGG	430	0.0	18.977156	29
TAGCGGC	440	0.0	18.182213	30
GATATAC	180	0.0	17.789713	1
TCTATAC	45	0.008855817	17.775854	3
TGGACGG	45	0.008858025	17.775085	5
CCAACGA	145	3.6379788E-12	17.655554	29
AATCCCG	100	6.8192094E-8	17.598858	19
CGCGGTC	155	0.0	17.548765	10
AACGAAT	120	1.6589183E-9	17.333708	31
CGCCGGT	495	0.0	17.1302	7
AAGACGG	395	0.0	17.01008	5
CCGGTCC	510	0.0	16.941544	9
AGAACCG	210	0.0	16.759365	5
AGCGGCG	490	0.0	16.653421	31
GTCCTAC	250	0.0	16.65117	1
CAAGACG	425	0.0	16.185785	4
GTCTTAA	90	8.526384E-6	16.01074	1
>>END_MODULE