FastQCFastQC Report
Thu 2 Feb 2017
SRR4063036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155494
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36000.31155505783673476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25380.21964631577489802No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21010.18182699347638326No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19740.17083602338047624No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16300.1410652067427438No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16140.13968051759680275No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15890.13751694080626986No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15650.1354399070873583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15600.13500719172925174No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15260.13206472729412702No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14960.12946843514548756No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14450.1250547384928005No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13380.11579462982931976No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13150.1138041391820295No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12520.10835192566988663No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG12180.10540946123476193No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC11930.10324588444422904No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG11770.10186119529828801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACG308.4136357E-426.6684325
CTAGCGG2900.022.62023229
TAGCGGC3000.021.86622230
TCTAGCG3050.021.50776128
AGCGGCG3800.017.68385131
TAAGTAG550.001364045917.4557025
TCTAAGC854.9808314E-616.94233
GCGGCGC4150.016.19244432
GCATTCG4450.016.17944722
ACCGTCC703.694554E-416.001068
TACCGTC3100.016.001067
TATGATA600.002440837216.0010594
GTATTAG2100.016.0003681
GTTTAGA1102.0186599E-716.0003681
CGGTCCA4350.015.81577110
CCGTTCT4550.015.4728839
TATAGAG1153.3422657E-715.3053625
TATAGCC951.432094E-515.1582439
ATTCGTA4650.015.13947724
CTTGCGC4550.015.1218813
Produced by FastQC (version 0.11.5)