##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063036_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155494
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1485252195165	32.0	32.0	32.0	32.0	32.0
2	31.211536364533263	32.0	32.0	32.0	32.0	32.0
3	31.320266483426135	32.0	32.0	32.0	32.0	32.0
4	31.435373961266784	32.0	32.0	32.0	32.0	32.0
5	31.324176499401986	32.0	32.0	32.0	32.0	32.0
6	34.87225636827193	36.0	36.0	36.0	36.0	36.0
7	34.90185582962785	36.0	36.0	36.0	36.0	36.0
8	34.820886131818945	36.0	36.0	36.0	32.0	36.0
9	34.965556722925434	36.0	36.0	36.0	32.0	36.0
10	34.77210007148458	36.0	36.0	36.0	32.0	36.0
11	34.9778371847885	36.0	36.0	36.0	36.0	36.0
12	34.86489674546125	36.0	36.0	36.0	32.0	36.0
13	34.92840637857055	36.0	36.0	36.0	36.0	36.0
14	34.869667864999734	36.0	36.0	36.0	32.0	36.0
15	34.838485530863856	36.0	36.0	36.0	32.0	36.0
16	34.85208231284628	36.0	36.0	36.0	32.0	36.0
17	34.806862692493425	36.0	36.0	36.0	32.0	36.0
18	34.81669485086032	36.0	36.0	36.0	32.0	36.0
19	34.8074537816726	36.0	36.0	36.0	32.0	36.0
20	34.79067308008523	36.0	36.0	36.0	32.0	36.0
21	34.78640564122358	36.0	36.0	36.0	32.0	36.0
22	34.772264503320656	36.0	36.0	36.0	32.0	36.0
23	34.70892362920102	36.0	36.0	36.0	32.0	36.0
24	34.69427102174481	36.0	36.0	36.0	32.0	36.0
25	34.66516052874355	36.0	36.0	36.0	32.0	36.0
26	34.60011908326655	36.0	36.0	36.0	32.0	36.0
27	34.59287888989471	36.0	36.0	36.0	32.0	36.0
28	34.56370435502045	36.0	36.0	36.0	32.0	36.0
29	34.51886812047488	36.0	36.0	36.0	32.0	36.0
30	34.50210213120968	36.0	36.0	36.0	32.0	36.0
31	34.49924534441546	36.0	36.0	36.0	32.0	36.0
32	34.466211853977605	36.0	36.0	36.0	32.0	36.0
33	34.43840037248138	36.0	36.0	36.0	32.0	36.0
34	34.419992661147525	36.0	36.0	36.0	32.0	36.0
35	34.39615696836158	36.0	36.0	36.0	32.0	36.0
36	34.36436277470934	36.0	36.0	36.0	32.0	36.0
37	34.36389544212259	36.0	36.0	36.0	32.0	36.0
38	33.911621349829595	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	20.0
22	78.0
23	282.0
24	764.0
25	1996.0
26	4094.0
27	8034.0
28	13702.0
29	21568.0
30	33286.0
31	48223.0
32	70418.0
33	113368.0
34	273335.0
35	566321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30527383542698	18.34772659212984	11.921911161656288	26.425088410786895
2	16.32081170477735	19.91468583999571	36.239478526067636	27.525023929159303
3	18.548689997524868	23.414401113290072	28.233984771881115	29.80292411730394
4	12.130805339389072	15.48653036639065	35.31347794178736	37.06918635243291
5	14.493108575206795	36.6790307868323	32.712329099069315	16.11553153889159
6	35.554143941898445	35.30983285071147	15.729203267174041	13.406819940216048
7	31.153303925870347	30.168699139498518	19.758647637493738	18.9193492971374
8	28.818749080460766	32.76900308965183	18.478887379161726	19.93336045072568
9	27.218258419186885	13.883782679738562	18.12816758059156	40.769791320482994
10	16.09869448759612	26.278662206778975	30.64496726482819	26.977676040796723
11	38.68864632449162	20.80494568265376	21.372698696245212	19.133709296609407
12	25.186522439378585	23.502216806535056	27.75426204098517	23.556998713101184
13	29.68562309388555	18.779705960750917	25.07278284921055	26.46188809615298
14	23.654125421681115	19.516241538251172	24.42288752689326	32.40674551317445
15	25.592949855213444	27.0970684399919	21.73183071482846	25.578150989966197
16	26.341201252451334	25.509349248027252	23.13780945638835	25.011640043133067
17	24.39727077769335	25.843924762915254	24.511334546090243	25.247469913301153
18	25.57001594123379	24.173037679122523	25.836482058755823	24.420464320887863
19	25.754612313002056	24.595194782491298	25.13418503254885	24.516007871957797
20	26.078196857794154	23.86156916435741	24.60843587244936	25.451798105399075
21	27.42846559866654	23.680108836661063	24.199281172625017	24.69214439204738
22	26.218050461534204	23.930284363224732	24.88398901249163	24.967676162749438
23	24.54484795667304	23.567516203040604	25.59807804980212	26.289557790484235
24	25.089009549162522	24.667717876509958	25.03292963875191	25.210342935575607
25	25.131286066887526	24.187878410235637	25.28758312476417	25.393252398112665
26	24.7754640006785	25.36430305998993	25.578150989966197	24.28208194936538
27	25.684945140346898	24.338421488990853	24.81510072748106	25.161532643181182
28	24.717393599620596	24.26685036876003	25.722851005717036	25.29290502590234
29	24.46036067690529	24.503372583501083	25.690916612288774	25.34535012730486
30	24.402117189704146	24.8676323719552	25.865733616963826	24.86451682137683
31	25.295241688836118	24.58930985362105	24.5217197146848	25.593728742858033
32	25.060277249384242	24.567933714930586	24.44582144087291	25.92596759481226
33	24.594242808703466	24.193634930168393	25.199784680837805	26.012337580290335
34	25.538341177020392	24.422714440750017	25.316704370598202	24.72224001163139
35	26.09740941969409	24.243829911708758	25.273779007074033	24.384981661523124
36	24.53219142635098	25.087105601586853	24.91159625233883	25.46910671972334
37	25.832760706676105	24.79277261500276	24.558587063195482	24.815879615125652
38	24.85209763460067	24.39601485774025	25.19134706254998	25.560540445109098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	27.5
2	37.0
3	37.0
4	170.5
5	304.0
6	304.0
7	501.0
8	698.0
9	821.0
10	944.0
11	944.0
12	1221.0
13	1498.0
14	2052.5
15	2607.0
16	2607.0
17	3848.5
18	5090.0
19	5090.0
20	5945.5
21	6801.0
22	7163.0
23	7525.0
24	7525.0
25	8712.5
26	9900.0
27	9900.0
28	13115.5
29	16331.0
30	20054.0
31	23777.0
32	23777.0
33	30833.0
34	37889.0
35	37889.0
36	42342.0
37	46795.0
38	53740.0
39	60685.0
40	60685.0
41	64630.5
42	68576.0
43	78607.0
44	88638.0
45	88638.0
46	89725.0
47	90812.0
48	90812.0
49	97400.5
50	103989.0
51	106832.0
52	109675.0
53	109675.0
54	105687.5
55	101700.0
56	101700.0
57	98750.5
58	95801.0
59	86675.5
60	77550.0
61	77550.0
62	73744.0
63	69938.0
64	58628.0
65	47318.0
66	47318.0
67	40059.5
68	32801.0
69	32801.0
70	26195.0
71	19589.0
72	15329.5
73	11070.0
74	11070.0
75	8274.0
76	5478.0
77	5478.0
78	5253.0
79	5028.0
80	3917.5
81	2807.0
82	2807.0
83	2602.5
84	2398.0
85	2398.0
86	1587.5
87	777.0
88	627.0
89	477.0
90	477.0
91	292.5
92	108.0
93	74.0
94	40.0
95	40.0
96	27.0
97	14.0
98	14.0
99	12.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007961962589160999
2	0.0
3	0.0
4	5.192584297278913E-4
5	0.0
6	0.0
7	4.327153581065761E-4
8	0.002077033718911565
9	0.003288636721609978
10	7.78887644591837E-4
11	0.005365670440521543
12	2.5962921486394564E-4
13	1.730861432426304E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	8.65430716213152E-5
22	0.0
23	8.65430716213152E-5
24	0.0
25	1.730861432426304E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.730861432426304E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1155494.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.6009488417409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88138399912944	37.890690809387046
2	14.915890732221904	15.095164477403156
3	4.866590967985251	7.387623618140999
4	2.071054300398149	4.191892508116569
5	0.9992216735533787	2.5280782392516623
6	0.5622902522500692	1.7071452172989203
7	0.34511025188651306	1.2224034340328835
8	0.23254854565174995	0.9413741649396359
9	0.15458791911615327	0.7040065848072887
>10	0.772613105947041	7.03235949205252
>50	0.08603650768000758	3.0589415801902153
>100	0.09598600657759664	10.554362741825853
>500	0.01152520019994587	3.950693903039933
>1k	0.005160537402960838	3.735263229513365
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3600	0.31155505783673476	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2538	0.21964631577489802	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2101	0.18182699347638326	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1974	0.17083602338047624	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1630	0.1410652067427438	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1614	0.13968051759680275	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1589	0.13751694080626986	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1565	0.1354399070873583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1560	0.13500719172925174	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1526	0.13206472729412702	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1496	0.12946843514548756	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1445	0.1250547384928005	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1338	0.11579462982931976	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1315	0.1138041391820295	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1252	0.10835192566988663	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1218	0.10540946123476193	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1193	0.10324588444422904	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1177	0.10186119529828801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	8.654307162131522E-5	0.0
7	0.0	0.0	0.0	8.654307162131522E-5	0.0
8	0.0	0.0	0.0	8.654307162131522E-5	0.0
9	0.0	0.0	0.0	8.654307162131522E-5	0.0
10	8.654307162131522E-5	0.0	0.0	8.654307162131522E-5	0.0
11	8.654307162131522E-5	0.0	0.0	8.654307162131522E-5	0.0
12	8.654307162131522E-5	0.0	0.0	8.654307162131522E-5	0.0
13	8.654307162131522E-5	0.0	0.0	8.654307162131522E-5	8.654307162131522E-5
14	8.654307162131522E-5	0.0	0.0	8.654307162131522E-5	8.654307162131522E-5
15	8.654307162131522E-5	0.0	0.0	8.654307162131522E-5	1.7308614324263043E-4
16	8.654307162131522E-5	0.0	0.0	2.5962921486394564E-4	2.5962921486394564E-4
17	8.654307162131522E-5	0.0	0.0	3.4617228648526087E-4	2.5962921486394564E-4
18	8.654307162131522E-5	0.0	0.0	3.4617228648526087E-4	2.5962921486394564E-4
19	8.654307162131522E-5	0.0	0.0	4.3271535810657604E-4	2.5962921486394564E-4
20	8.654307162131522E-5	0.0	0.0	6.058015013492065E-4	3.4617228648526087E-4
21	8.654307162131522E-5	0.0	0.0	7.78887644591837E-4	4.3271535810657604E-4
22	8.654307162131522E-5	0.0	0.0	9.519737878344673E-4	4.3271535810657604E-4
23	8.654307162131522E-5	0.0	0.0	0.0012981460743197281	4.3271535810657604E-4
24	8.654307162131522E-5	0.0	0.0	0.002077033718911565	4.3271535810657604E-4
25	8.654307162131522E-5	0.0	0.0	0.0022501198621541953	4.3271535810657604E-4
26	8.654307162131522E-5	0.0	0.0	0.002942464435124717	5.192584297278913E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTACG	30	8.4136357E-4	26.668432	5
CTAGCGG	290	0.0	22.620232	29
TAGCGGC	300	0.0	21.866222	30
TCTAGCG	305	0.0	21.507761	28
AGCGGCG	380	0.0	17.683851	31
TAAGTAG	55	0.0013640459	17.455702	5
TCTAAGC	85	4.9808314E-6	16.9423	3
GCGGCGC	415	0.0	16.192444	32
GCATTCG	445	0.0	16.179447	22
ACCGTCC	70	3.694554E-4	16.00106	8
TACCGTC	310	0.0	16.00106	7
TATGATA	60	0.0024408372	16.001059	4
GTATTAG	210	0.0	16.000368	1
GTTTAGA	110	2.0186599E-7	16.000368	1
CGGTCCA	435	0.0	15.815771	10
CCGTTCT	455	0.0	15.472883	9
TATAGAG	115	3.3422657E-7	15.305362	5
TATAGCC	95	1.432094E-5	15.158243	9
ATTCGTA	465	0.0	15.139477	24
CTTGCGC	455	0.0	15.121881	3
>>END_MODULE