FastQCFastQC Report
Thu 2 Feb 2017
SRR4063035_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063035_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308456
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33420.25541554320512116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21480.16416295236523046No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18620.142305129098724No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16390.1252621410272871No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16080.12289293640749097No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16020.1224343806746272No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15550.1188423607671943No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15300.11693171188026193No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15290.11685528592478463No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15220.11632030423644356No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15210.11624387828096626No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14330.10951839419896428No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14250.10890698655514591No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13860.10592637429153139No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13530.10340431776078064No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13150.10050013145264342No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13120.10027085358621153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA2750.018.0488451
CCGTAAA450.00881570517.7900811
AACGGAC550.001364400117.45527515
GTATTAG3100.016.527561
GTATAGG1001.305254E-616.0110741
GTATCAA28150.015.7551231
TATAGTA1451.2169039E-915.4518752
GACGGCC5700.015.43924111
GGACCGT859.4488656E-515.0560016
ATCGTTT3300.015.02978329
GCATTCG5750.015.02614222
TAGGTAC756.2558753E-414.9299635
TAGGAGT1501.9790605E-914.9299634
ACATCGC650.00416717814.7664626
TAAGAGG6300.014.7268344
ATTCGTA5900.014.64412124
CATCGTT3400.014.58772928
CGTATTG5950.014.5199527
TACCGTC3100.014.4511137
GTATAGA1002.31104E-514.4099661
Produced by FastQC (version 0.11.5)