##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063035_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1308456
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205895345353607	32.0	32.0	32.0	32.0	32.0
2	30.760548310374976	32.0	32.0	32.0	32.0	32.0
3	30.818356138838446	32.0	32.0	32.0	32.0	32.0
4	30.896048472398	32.0	32.0	32.0	32.0	32.0
5	30.75395045763862	32.0	32.0	32.0	32.0	32.0
6	34.43631272278166	36.0	36.0	36.0	32.0	36.0
7	34.35858599754214	36.0	36.0	36.0	32.0	36.0
8	34.33070657324358	36.0	36.0	36.0	32.0	36.0
9	34.505628007361345	36.0	36.0	36.0	32.0	36.0
10	34.16684091784516	36.0	36.0	36.0	32.0	36.0
11	34.505742646294564	36.0	36.0	36.0	32.0	36.0
12	34.277666960142334	36.0	36.0	36.0	32.0	36.0
13	34.39050988340456	36.0	36.0	36.0	32.0	36.0
14	34.277997884529555	36.0	36.0	36.0	32.0	36.0
15	34.20456400520919	36.0	36.0	36.0	32.0	36.0
16	34.231368116314194	36.0	36.0	36.0	32.0	36.0
17	34.145773338958286	36.0	36.0	36.0	32.0	36.0
18	34.18261370653656	36.0	36.0	36.0	32.0	36.0
19	34.16399481526318	36.0	36.0	36.0	32.0	36.0
20	34.15087477148639	36.0	36.0	36.0	32.0	36.0
21	34.13548105553416	36.0	36.0	36.0	32.0	36.0
22	34.10128349749628	36.0	36.0	36.0	32.0	36.0
23	34.04345426976528	36.0	36.0	36.0	32.0	36.0
24	34.03821756329597	36.0	36.0	36.0	32.0	36.0
25	33.99880469805633	36.0	36.0	36.0	32.0	36.0
26	33.95303242906143	36.0	36.0	36.0	32.0	36.0
27	33.96719721564959	36.0	36.0	36.0	32.0	36.0
28	33.946820527400234	36.0	36.0	36.0	32.0	36.0
29	33.9098983840496	36.0	36.0	36.0	32.0	36.0
30	33.883232603924014	36.0	36.0	36.0	32.0	36.0
31	33.89927823327647	36.0	36.0	36.0	32.0	36.0
32	33.851103896500916	36.0	36.0	36.0	32.0	36.0
33	33.80821135750839	36.0	36.0	36.0	32.0	36.0
34	33.81390203415323	36.0	36.0	36.0	32.0	36.0
35	33.78342030607067	36.0	36.0	36.0	32.0	36.0
36	33.72047971043734	36.0	36.0	36.0	27.0	36.0
37	33.72369189334605	36.0	36.0	36.0	27.0	36.0
38	33.10799293212764	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	2.0
9	5.0
10	9.0
11	11.0
12	5.0
13	5.0
14	124.0
15	334.0
16	421.0
17	509.0
18	665.0
19	846.0
20	1185.0
21	1740.0
22	2557.0
23	3942.0
24	5959.0
25	9259.0
26	13351.0
27	19346.0
28	27422.0
29	36429.0
30	49861.0
31	67174.0
32	92267.0
33	138283.0
34	298597.0
35	538141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.855031876765615	17.77852763928295	11.563093959176927	25.803346524774508
2	16.371732168582938	20.301311873541387	38.201702468671336	25.12525348920434
3	18.560288740231627	24.818696572050534	28.9790446257139	27.64197006200394
4	12.357262029502557	15.716634223179096	37.106254905692246	34.819848841626104
5	14.33156992909189	36.87351637887155	33.803863185102415	14.991050506934137
6	32.912556211462096	36.44084765814871	17.3946006425914	13.251995487797794
7	29.230329487579255	30.846279890191187	21.763513637447495	18.159876984782063
8	27.361368095154027	33.19553638779408	20.273117673088784	19.16997784396311
9	27.73582829175239	14.162460678725699	18.97937410008162	39.12233692944029
10	15.834790530460516	26.44560578639732	32.096728337694735	25.622875345447433
11	37.0248482143403	21.31656099246116	22.973974797160555	18.68461599603798
12	24.77606647386474	24.000706193099486	29.128783941596915	22.094443391438862
13	29.34492315829864	19.641145509274054	25.51208691757231	25.50184441485499
14	23.60484677830491	20.31195421960617	25.44864342640597	30.63455557568295
15	24.745711922529473	27.68777873076882	22.67096888611655	24.89554046058516
16	25.262169879967473	25.91079192119311	24.4090094717468	24.41802872709262
17	23.71413305121606	26.013011742061437	25.69066115885684	24.58219404786566
18	24.721488492674933	24.76337222099934	26.990470687504587	23.52466859882114
19	25.50739015654689	24.969618500078724	25.764276247705148	23.758715095669242
20	25.56772670853027	24.517952026023103	25.335377597877063	24.57894366756957
21	26.87150090761441	24.399197477787332	24.575293780452853	24.15400783414541
22	25.695201859425648	24.637007676016022	25.330317855698848	24.337472608859482
23	23.887827323172875	24.40718773407524	25.975434520078878	25.729550422673004
24	24.576620978319692	25.250098602444115	25.678140220319136	24.495140198917053
25	24.55170927202585	24.754958352665817	25.823946089691013	24.869386285617317
26	24.14438888961082	25.85977708591159	26.21109494831515	23.78473907616244
27	25.105706467737743	25.012534967974688	25.15706926334133	24.72468930094624
28	23.986438201105027	25.179897904356608	26.013752874736028	24.819911019802333
29	24.172839921365526	25.175032025510347	25.911695196632046	24.740432856492085
30	23.93929157195942	25.48008358185228	26.28655368544303	24.29407116074527
31	24.89819502531367	25.14491158558955	24.96866363391787	24.98822975517891
32	24.374027411332015	25.285853172358763	24.928995294887354	25.411124121421867
33	23.886855358266143	24.8484533490071	25.923400820732788	25.341290471993965
34	24.867422360485826	24.979859205527827	25.962668827247782	24.19004960673857
35	25.562948836036064	24.77792768174157	25.82039296920684	23.83873051301553
36	24.025221074226977	25.73266547985853	25.345971897259815	24.896141548654676
37	25.114439694542128	25.500809810361446	25.086312255843513	24.298438239252913
38	24.206636425822676	25.298746164164214	25.748613749011184	24.74600366100192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	561.0
1	409.5
2	258.0
3	258.0
4	562.0
5	866.0
6	866.0
7	1022.5
8	1179.0
9	1177.0
10	1175.0
11	1175.0
12	1568.5
13	1962.0
14	2564.0
15	3166.0
16	3166.0
17	4589.0
18	6012.0
19	6012.0
20	7474.5
21	8937.0
22	9819.0
23	10701.0
24	10701.0
25	13195.5
26	15690.0
27	15690.0
28	19977.5
29	24265.0
30	29901.5
31	35538.0
32	35538.0
33	44706.5
34	53875.0
35	53875.0
36	58916.5
37	63958.0
38	71825.0
39	79692.0
40	79692.0
41	82887.5
42	86083.0
43	95217.0
44	104351.0
45	104351.0
46	104822.0
47	105293.0
48	105293.0
49	111370.5
50	117448.0
51	116804.5
52	116161.0
53	116161.0
54	109935.5
55	103710.0
56	103710.0
57	101071.0
58	98432.0
59	87093.0
60	75754.0
61	75754.0
62	72249.5
63	68745.0
64	56717.0
65	44689.0
66	44689.0
67	37624.0
68	30559.0
69	30559.0
70	24585.5
71	18612.0
72	14463.0
73	10314.0
74	10314.0
75	7909.0
76	5504.0
77	5504.0
78	5336.0
79	5168.0
80	4137.0
81	3106.0
82	3106.0
83	3140.5
84	3175.0
85	3175.0
86	2119.0
87	1063.0
88	932.0
89	801.0
90	801.0
91	591.5
92	382.0
93	351.0
94	320.0
95	320.0
96	297.5
97	275.0
98	275.0
99	475.5
100	676.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11830737907885325
2	0.05410957647792512
3	0.011616745232548896
4	0.0020635007978869755
5	1.5285191095459075E-4
6	3.057038219091815E-4
7	0.0
8	6.878335992956583E-4
9	3.057038219091815E-4
10	6.11407643818363E-4
11	0.002445630575273452
12	0.002445630575273452
13	0.013909523896867757
14	0.009400392523707331
15	0.022469230910324843
16	0.011005337588730534
17	0.01933576673575573
18	0.005502668794365267
19	0.007184039814865766
20	0.0061140764381836295
21	0.0061905023936609255
22	0.00726046577034306
23	0.008865410835366265
24	0.013145264342094806
25	0.015973024694754735
26	0.018265803359073594
27	0.008865410835366265
28	0.005731946660797153
29	0.009476818479184626
30	0.002904186308137224
31	0.005502668794365267
32	0.007031187903911175
33	0.008636132968934377
34	0.012686708609231033
35	0.016125876605709327
36	0.014138801763299645
37	0.009553244434661922
38	0.005426242838887972
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1308456.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.63168513904551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8080031101305	48.551727653462734
2	11.280427743554581	13.227809753876919
3	2.8952094309233614	5.09253023296481
4	1.0512126977939664	2.4653748764488976
5	0.5243779969197817	1.537258280462201
6	0.29867869060571656	1.0507220967202058
7	0.19372351969395213	0.7950835487506438
8	0.14220631362344668	0.6670236644123427
9	0.09915662526958165	0.523234802903079
>10	0.5439600077831904	5.914551294168485
>50	0.07096713151284947	2.935864619606467
>100	0.07782055051915412	9.924856200827085
>500	0.010463252601776118	4.110840278285729
>1k	0.003792929068143843	3.2031226971103575
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3342	0.25541554320512116	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2148	0.16416295236523046	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1862	0.142305129098724	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1639	0.1252621410272871	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1608	0.12289293640749097	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1602	0.1224343806746272	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1555	0.1188423607671943	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1530	0.11693171188026193	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1529	0.11685528592478463	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1522	0.11632030423644356	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1521	0.11624387828096626	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1433	0.10951839419896428	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1425	0.10890698655514591	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1386	0.10592637429153139	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1353	0.10340431776078064	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1315	0.10050013145264342	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1312	0.10027085358621153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.642595547729537E-5	0.0
5	0.0	0.0	0.0	7.642595547729537E-5	0.0
6	0.0	0.0	0.0	7.642595547729537E-5	0.0
7	0.0	0.0	0.0	7.642595547729537E-5	0.0
8	0.0	0.0	0.0	7.642595547729537E-5	0.0
9	0.0	0.0	0.0	1.5285191095459075E-4	0.0
10	0.0	0.0	0.0	1.5285191095459075E-4	0.0
11	0.0	0.0	0.0	1.5285191095459075E-4	0.0
12	0.0	0.0	0.0	1.5285191095459075E-4	3.057038219091815E-4
13	0.0	0.0	0.0	1.5285191095459075E-4	3.821297773864769E-4
14	0.0	0.0	0.0	1.5285191095459075E-4	3.821297773864769E-4
15	0.0	0.0	0.0	1.5285191095459075E-4	5.349816883410677E-4
16	0.0	0.0	0.0	1.5285191095459075E-4	6.11407643818363E-4
17	0.0	0.0	0.0	1.5285191095459075E-4	6.11407643818363E-4
18	0.0	0.0	0.0	2.2927786643188612E-4	6.11407643818363E-4
19	0.0	0.0	0.0	3.057038219091815E-4	6.11407643818363E-4
20	0.0	0.0	0.0	4.5855573286377225E-4	6.878335992956583E-4
21	0.0	0.0	0.0	6.11407643818363E-4	6.878335992956583E-4
22	0.0	0.0	0.0	6.878335992956583E-4	6.878335992956583E-4
23	0.0	0.0	0.0	0.0014520931540686123	6.878335992956583E-4
24	0.0	0.0	0.0	0.0025220565307507476	6.878335992956583E-4
25	0.0	0.0	0.0	0.0031334641745691103	6.878335992956583E-4
26	0.0	0.0	0.0	0.003668445862910178	6.878335992956583E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	275	0.0	18.048845	1
CCGTAAA	45	0.008815705	17.790081	1
AACGGAC	55	0.0013644001	17.455275	15
GTATTAG	310	0.0	16.52756	1
GTATAGG	100	1.305254E-6	16.011074	1
GTATCAA	2815	0.0	15.755123	1
TATAGTA	145	1.2169039E-9	15.451875	2
GACGGCC	570	0.0	15.439241	11
GGACCGT	85	9.4488656E-5	15.056001	6
ATCGTTT	330	0.0	15.029783	29
GCATTCG	575	0.0	15.026142	22
TAGGTAC	75	6.2558753E-4	14.929963	5
TAGGAGT	150	1.9790605E-9	14.929963	4
ACATCGC	65	0.004167178	14.766462	6
TAAGAGG	630	0.0	14.726834	4
ATTCGTA	590	0.0	14.644121	24
CATCGTT	340	0.0	14.587729	28
CGTATTG	595	0.0	14.51995	27
TACCGTC	310	0.0	14.451113	7
GTATAGA	100	2.31104E-5	14.409966	1
>>END_MODULE