FastQCFastQC Report
Thu 2 Feb 2017
SRR4063035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308456
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38690.2956920217416558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27150.20749646912085695No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19450.1486484834033395No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17520.1338982739962215No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17450.13336329230788044No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17320.13236975488667557No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16300.12457430742799146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16280.12442145551703687No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15860.12121156538699047No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15640.11953019436648997No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15550.1188423607671943No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15510.11853665694528515No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15240.11647315614739814No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14660.11204045072971502No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14240.10883056059966861No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14170.10829557891132754No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13940.10653778193534975No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13600.10393929944912171No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13560.10363359562721253No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13490.10309861393887146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2650.018.7185921
TATACCG753.2318596E-517.0681345
TGAACCG600.002440747316.0013755
TCTAGAT2300.015.3039082
AACCGCG2200.015.2740417
ATACCGT3700.015.1364366
TGGATAC5100.015.05839124
TACCGTG650.0041596114.7705017
TACGCTA4400.014.5467059
CGACCCG3200.014.5012465
TATTAGC2003.6379788E-1214.3995872
ATTAGAG4700.014.2974623
GTATCAA28250.014.2737941
AACGAAT901.5348144E-414.22181531
ATACGCT4400.014.1830388
GTATAAT2150.014.1407521
ACCGCAG5450.014.09133929
ACAATAC2500.014.07959653
GTCCTAT4800.014.0012041
ATACCGC5600.013.999598527
Produced by FastQC (version 0.11.5)