FastQCFastQC Report
Thu 2 Feb 2017
SRR4063034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968646
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT22540.23269594877798494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT14160.14618343543461698No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9980.10303041565236423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG350.002068750822.8524955
AGGCGTA400.00448423419.99903320
CTTATAC1105.2023097E-1018.9081753
GTCTTAG701.7685505E-518.3075141
CATCGTT2400.017.99912828
TAGGACG2700.017.775084
AACCGCG1453.6379788E-1217.6534067
TCCTATA1051.1828706E-716.7688852
ATCGTTT2500.016.63919429
ACGTTTT1551.0913936E-1116.51533129
GACGTGA1651.8189894E-1216.4831987
TCTAGAT2050.016.397072
GTCCTAC2850.016.3001141
GTCCTAT3250.016.2655221
GTCTATA600.00242219216.0190751
GCATCGT2600.015.99922727
TCTAGCG1501.1459633E-1015.99922628
TTAATAC703.6970098E-415.9992253
TACCGTC1501.1459633E-1015.99847
TAATACC908.590365E-615.9975744
Produced by FastQC (version 0.11.5)