##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063034_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	968646
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2673246985999	32.0	32.0	32.0	32.0	32.0
2	30.874678675181645	32.0	32.0	32.0	32.0	32.0
3	30.919401928052146	32.0	32.0	32.0	32.0	32.0
4	30.99034941557597	32.0	32.0	32.0	32.0	32.0
5	30.89572145035441	32.0	32.0	32.0	32.0	32.0
6	34.553278493897665	36.0	36.0	36.0	32.0	36.0
7	34.494746274696844	36.0	36.0	36.0	32.0	36.0
8	34.479187443090666	36.0	36.0	36.0	32.0	36.0
9	34.62324110149631	36.0	36.0	36.0	32.0	36.0
10	34.334007470221316	36.0	36.0	36.0	32.0	36.0
11	34.60953227494874	36.0	36.0	36.0	32.0	36.0
12	34.43157355731609	36.0	36.0	36.0	32.0	36.0
13	34.50305890903385	36.0	36.0	36.0	32.0	36.0
14	34.416427673267634	36.0	36.0	36.0	32.0	36.0
15	34.354377140874995	36.0	36.0	36.0	32.0	36.0
16	34.36273519944335	36.0	36.0	36.0	32.0	36.0
17	34.309467029234625	36.0	36.0	36.0	32.0	36.0
18	34.324241260481124	36.0	36.0	36.0	32.0	36.0
19	34.316574889071966	36.0	36.0	36.0	32.0	36.0
20	34.29121268244539	36.0	36.0	36.0	32.0	36.0
21	34.276869981396715	36.0	36.0	36.0	32.0	36.0
22	34.24860165633265	36.0	36.0	36.0	32.0	36.0
23	34.19952387146594	36.0	36.0	36.0	32.0	36.0
24	34.18261160423932	36.0	36.0	36.0	32.0	36.0
25	34.15692007193547	36.0	36.0	36.0	32.0	36.0
26	34.12421049588807	36.0	36.0	36.0	32.0	36.0
27	34.119987074741445	36.0	36.0	36.0	32.0	36.0
28	34.09636337733289	36.0	36.0	36.0	32.0	36.0
29	34.06992440994956	36.0	36.0	36.0	32.0	36.0
30	34.0359574085889	36.0	36.0	36.0	32.0	36.0
31	34.047582914707746	36.0	36.0	36.0	32.0	36.0
32	34.01812530067744	36.0	36.0	36.0	32.0	36.0
33	33.97311608162322	36.0	36.0	36.0	32.0	36.0
34	33.97534496606603	36.0	36.0	36.0	32.0	36.0
35	33.93740540920006	36.0	36.0	36.0	32.0	36.0
36	33.89437420894733	36.0	36.0	36.0	32.0	36.0
37	33.89419251202194	36.0	36.0	36.0	32.0	36.0
38	33.297152933063266	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	3.0
10	12.0
11	8.0
12	4.0
13	4.0
14	124.0
15	262.0
16	361.0
17	423.0
18	471.0
19	608.0
20	744.0
21	1127.0
22	1681.0
23	2529.0
24	3944.0
25	6011.0
26	8737.0
27	12641.0
28	18251.0
29	24928.0
30	33650.0
31	45834.0
32	63217.0
33	95799.0
34	214084.0
35	433186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.749778795822344	16.966980624994832	11.229523108600914	26.053717470581912
2	16.671556130354478	19.588499981406265	37.704479437394895	26.035464450844366
3	19.139643727756216	23.66692169258742	28.352584176622486	28.84085040303388
4	12.7235129420704	15.278993706536282	35.93458349163349	36.06290985975982
5	14.58380435310016	36.48320382225443	33.794597183895405	15.138394640749997
6	34.28335458294834	35.31809899395547	16.86985428098013	13.52869214211605
7	30.2411100041811	30.415167579453772	20.899400709238165	18.444321707126964
8	28.03176399172451	33.30222455308658	19.471343445063408	19.194668010125497
9	27.68749683836047	14.183243981528799	18.452515529457187	39.67674365065355
10	15.79634168247918	26.6087987099465	31.95535605517828	25.639503552396036
11	37.55749117049184	20.887916607734454	22.32686059752619	19.227731624247518
12	24.975945159092316	23.562697445850798	28.837727901550657	22.623629493506225
13	29.93779007635558	19.40206196211648	24.929091012343893	25.731056949184044
14	23.633065373742713	19.672005897451253	25.17102815124402	31.523900577562014
15	25.267834001261853	27.109705839529873	22.302272677988924	25.320187481219353
16	25.54084037916904	25.53929165475009	24.09237005427763	24.827497911803242
17	24.01802051108367	25.832565785397726	25.309669451867517	24.839744251651087
18	24.853421708763726	24.644872211811954	26.459046350641906	24.04265972878241
19	25.68659577960794	24.9688462423974	25.337942127830036	24.00661585016462
20	25.841308057870226	24.195085387799264	25.206255336414817	24.757351217915694
21	26.724166411999374	24.13874274198211	24.42586290915398	24.711227936864535
22	25.81862445486983	24.593836725254395	24.744160499537465	24.84337832033831
23	24.382198223126203	24.395104046750056	25.439959527337113	25.782738202786625
24	24.891483465425456	25.020546898554073	25.17057023322279	24.91739940279768
25	24.940190337732282	24.493205395208413	25.310870851398416	25.25573341566089
26	24.606977904026714	25.19626581252446	25.82572070830197	24.371035575146855
27	25.240435990596282	24.9755046961808	24.754350045893204	25.029709267329718
28	24.48427983980954	24.743520783153365	25.469725799274624	25.302473577762473
29	24.55210566093841	25.00660793861225	25.455947764245266	24.98533863620407
30	24.56571967780767	25.139116304327626	25.612679560691877	24.682484457172826
31	25.09792625538672	24.784273156952505	24.760010985085476	25.3577896025753
32	24.549677208871103	25.146838216239598	24.791680217389732	25.511804357499564
33	24.287764436230734	24.83537586338623	25.40012595888784	25.476733741495195
34	24.974471279285755	24.789239631312615	25.597588891057153	24.63870019834448
35	25.518491825351425	24.81904671434265	25.314564442085658	24.347897018220266
36	24.540713444218095	25.38448115822916	25.106322785548567	24.968482612004177
37	25.2951592336959	25.037659582156742	24.86120933978287	24.805971844364485
38	24.66827864181114	25.201634551055974	25.163847430888804	24.966239376244083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	281.0
1	240.0
2	199.0
3	199.0
4	444.5
5	690.0
6	690.0
7	763.0
8	836.0
9	794.5
10	753.0
11	753.0
12	978.5
13	1204.0
14	1510.0
15	1816.0
16	1816.0
17	2754.5
18	3693.0
19	3693.0
20	4556.0
21	5419.0
22	5899.5
23	6380.0
24	6380.0
25	7711.0
26	9042.0
27	9042.0
28	11710.5
29	14379.0
30	18249.0
31	22119.0
32	22119.0
33	28159.5
34	34200.0
35	34200.0
36	38012.5
37	41825.0
38	47424.5
39	53024.0
40	53024.0
41	56962.0
42	60900.0
43	68314.5
44	75729.0
45	75729.0
46	79817.5
47	83906.0
48	83906.0
49	87591.5
50	91277.0
51	91249.5
52	91222.0
53	91222.0
54	87694.0
55	84166.0
56	84166.0
57	81194.0
58	78222.0
59	70518.5
60	62815.0
61	62815.0
62	57700.0
63	52585.0
64	43675.0
65	34765.0
66	34765.0
67	28540.5
68	22316.0
69	22316.0
70	17871.5
71	13427.0
72	10642.0
73	7857.0
74	7857.0
75	6013.0
76	4169.0
77	4169.0
78	3849.5
79	3530.0
80	2744.0
81	1958.0
82	1958.0
83	1931.5
84	1905.0
85	1905.0
86	1249.0
87	593.0
88	541.0
89	489.0
90	489.0
91	347.5
92	206.0
93	186.5
94	167.0
95	167.0
96	152.0
97	137.0
98	137.0
99	291.0
100	445.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1253295837695092
2	0.059670922091249017
3	0.012078716063453522
4	0.002271211567486987
5	3.0971066829368004E-4
6	1.0323688943122668E-4
7	1.0323688943122668E-4
8	2.0647377886245336E-4
9	3.0971066829368004E-4
10	4.129475577249067E-4
11	0.001961500899193307
12	0.0026841591252118936
13	0.01558877030411523
14	0.009497793827672855
15	0.023641247679750912
16	0.011046347169141255
17	0.020027956549657975
18	0.005265081360992561
19	0.0065039240341672805
20	0.006710397813029734
21	0.006400687144736054
22	0.007226582260185867
23	0.009394556938241629
24	0.013420795626059467
25	0.016208191640702592
26	0.019511772102501844
27	0.009601030717104081
28	0.005058607582130108
29	0.011769005395159841
30	0.0033035804617992538
31	0.006194213365873601
32	0.0065039240341672805
33	0.007846003596773229
34	0.013317558736628243
35	0.016930849866721175
36	0.01373050629435315
37	0.010426925832553896
38	0.006400687144736054
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	968646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.14564552799256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.38920158513348	53.490541657422874
2	10.73133950696227	13.767374001082883
3	2.784768676981864	5.358923532934064
4	1.058357066665638	2.7155598896152
5	0.5568221334056382	1.7858857595789326
6	0.3183266189024777	1.2251559874945628
7	0.22200730053836137	0.9968561123472169
8	0.1611159114891314	0.8267907317840998
9	0.10429198735686677	0.6020889167163112
>10	0.5499338600186717	6.559971339990016
>50	0.05877517041097969	2.691422085712162
>100	0.06037843229642832	7.608089568859225
>500	0.004358870539020163	1.991288041755437
>1k	3.228792991866787E-4	0.38005237470708064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2254	0.23269594877798494	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1416	0.14618343543461698	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	998	0.10303041565236423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0323688943122668E-4	0.0
10	0.0	0.0	0.0	1.0323688943122668E-4	0.0
11	0.0	0.0	0.0	1.0323688943122668E-4	0.0
12	0.0	0.0	0.0	1.0323688943122668E-4	0.0
13	0.0	0.0	0.0	1.0323688943122668E-4	0.0
14	0.0	0.0	0.0	1.0323688943122668E-4	0.0
15	0.0	0.0	0.0	1.0323688943122668E-4	0.0
16	0.0	0.0	0.0	2.0647377886245336E-4	0.0
17	0.0	0.0	0.0	3.0971066829368004E-4	0.0
18	0.0	0.0	0.0	3.0971066829368004E-4	0.0
19	0.0	0.0	0.0	4.129475577249067E-4	0.0
20	0.0	0.0	0.0	5.161844471561335E-4	0.0
21	0.0	0.0	0.0	5.161844471561335E-4	0.0
22	0.0	0.0	0.0	7.226582260185868E-4	0.0
23	0.0	0.0	0.0	0.0014453164520371736	0.0
24	0.0	0.0	0.0	0.002271211567486987	0.0
25	0.0	0.0	0.0	0.00278739601464312	0.0
26	0.0	0.0	0.0	0.003510054240661707	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	35	0.0020687508	22.852495	5
AGGCGTA	40	0.004484234	19.999033	20
CTTATAC	110	5.2023097E-10	18.908175	3
GTCTTAG	70	1.7685505E-5	18.307514	1
CATCGTT	240	0.0	17.999128	28
TAGGACG	270	0.0	17.77508	4
AACCGCG	145	3.6379788E-12	17.653406	7
TCCTATA	105	1.1828706E-7	16.768885	2
ATCGTTT	250	0.0	16.639194	29
ACGTTTT	155	1.0913936E-11	16.515331	29
GACGTGA	165	1.8189894E-12	16.483198	7
TCTAGAT	205	0.0	16.39707	2
GTCCTAC	285	0.0	16.300114	1
GTCCTAT	325	0.0	16.265522	1
GTCTATA	60	0.002422192	16.019075	1
GCATCGT	260	0.0	15.999227	27
TCTAGCG	150	1.1459633E-10	15.999226	28
TTAATAC	70	3.6970098E-4	15.999225	3
TACCGTC	150	1.1459633E-10	15.9984	7
TAATACC	90	8.590365E-6	15.997574	4
>>END_MODULE