FastQCFastQC Report
Thu 2 Feb 2017
SRR4063034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968646
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26220.27068712408867635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19260.19883424904454258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11000.11356057837434935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT756.394475E-821.3349864
CGCGTAA659.433408E-619.691810
GCGTAAC751.5084388E-619.19950511
ATTATAC852.3930806E-718.8249873
GAATAGA701.785597E-518.287131
CGAACGA802.7959504E-617.99953716
GTGTACG450.00884534917.7791561
GTATATA904.3614273E-717.7791561
GCGTTCG450.00885061217.7773213
CCTTAAC652.09956E-417.2312152
TATACTA753.2306954E-517.0679875
TCTAGCG1900.016.84167128
TTATACT1456.91216E-1116.5530074
CGTTTAT1850.016.43200931
TTTATAC908.571704E-616.001243
TAGTACT1001.3127847E-616.001244
CCACGCA805.618154E-516.0004149
TCGCGTA805.618154E-516.0004149
CGCATCG1900.015.99958713
TAACGAA1052.1845535E-615.23770213
Produced by FastQC (version 0.11.5)