##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063034_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	968646
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21551010379437	32.0	32.0	32.0	32.0	32.0
2	31.297791969408845	32.0	32.0	32.0	32.0	32.0
3	31.37633149778144	32.0	32.0	32.0	32.0	32.0
4	31.478267602405833	32.0	32.0	32.0	32.0	32.0
5	31.404369604582065	32.0	32.0	32.0	32.0	32.0
6	34.940734798884215	36.0	36.0	36.0	36.0	36.0
7	34.96612900894651	36.0	36.0	36.0	36.0	36.0
8	34.91032534073335	36.0	36.0	36.0	36.0	36.0
9	35.01049402981069	36.0	36.0	36.0	36.0	36.0
10	34.86831721805489	36.0	36.0	36.0	32.0	36.0
11	35.04353396390425	36.0	36.0	36.0	36.0	36.0
12	34.93211761572339	36.0	36.0	36.0	32.0	36.0
13	34.98640886350638	36.0	36.0	36.0	36.0	36.0
14	34.93565760866199	36.0	36.0	36.0	32.0	36.0
15	34.90487546534028	36.0	36.0	36.0	32.0	36.0
16	34.92139956186264	36.0	36.0	36.0	32.0	36.0
17	34.891325623602434	36.0	36.0	36.0	32.0	36.0
18	34.882882910784744	36.0	36.0	36.0	32.0	36.0
19	34.88512521602319	36.0	36.0	36.0	32.0	36.0
20	34.866148211007946	36.0	36.0	36.0	32.0	36.0
21	34.85182718970604	36.0	36.0	36.0	32.0	36.0
22	34.84103687002269	36.0	36.0	36.0	32.0	36.0
23	34.783776529299665	36.0	36.0	36.0	32.0	36.0
24	34.757715408931645	36.0	36.0	36.0	32.0	36.0
25	34.745788451095656	36.0	36.0	36.0	32.0	36.0
26	34.67961154023245	36.0	36.0	36.0	32.0	36.0
27	34.66590168131598	36.0	36.0	36.0	32.0	36.0
28	34.63807624250758	36.0	36.0	36.0	32.0	36.0
29	34.604268225956645	36.0	36.0	36.0	32.0	36.0
30	34.5808076428334	36.0	36.0	36.0	32.0	36.0
31	34.576617257491385	36.0	36.0	36.0	32.0	36.0
32	34.545397389758485	36.0	36.0	36.0	32.0	36.0
33	34.51492702184286	36.0	36.0	36.0	32.0	36.0
34	34.498641402535085	36.0	36.0	36.0	32.0	36.0
35	34.46145134548638	36.0	36.0	36.0	32.0	36.0
36	34.45065586395856	36.0	36.0	36.0	32.0	36.0
37	34.42208505480846	36.0	36.0	36.0	32.0	36.0
38	34.00844684229326	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	20.0
22	72.0
23	209.0
24	630.0
25	1442.0
26	3167.0
27	6082.0
28	10811.0
29	17303.0
30	26345.0
31	38393.0
32	55057.0
33	88468.0
34	217842.0
35	502803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.127648920298796	18.094171213818523	11.918992726277805	26.859187139604874
2	15.760040303681633	20.536191756327902	36.905639418322075	26.798128521668392
3	18.12282299209412	24.41108516423957	28.810525207351294	28.655566636315022
4	12.228769762203166	16.081173436625708	36.00422034146774	35.68583645970338
5	14.108161400719563	36.92766699874567	33.56286358779532	15.401308012739445
6	34.223615693691386	35.76783627421426	16.59722250899195	13.411325523102398
7	29.995674360935865	30.51640284397864	20.941151693658036	18.54677110142746
8	27.884595530998485	33.4380174845245	19.335802805617845	19.34158417885917
9	27.49147740764524	14.076369860892296	18.251356078141487	40.18079665332097
10	15.540838396299947	26.83714711939998	32.09299684607721	25.52901763822286
11	37.54258741518652	20.926268537140356	22.291543636356128	19.239600411317
12	24.80632719554346	23.577186252121525	28.911860291293806	22.70462626104121
13	29.92089973199648	19.332696016286686	25.022402451261765	25.72400180045507
14	23.531610103174945	19.51486920918478	25.190213968777037	31.763306718863237
15	25.166469484207855	27.217992950985188	22.306910883852304	25.308626680954653
16	25.54999452844486	25.526250043875677	24.029624857791184	24.894130569888276
17	24.038193519613976	25.776083316299246	25.376969501758122	24.80875366232865
18	24.891343173873633	24.62499199914107	26.4546593905307	24.029005436454597
19	25.657360893453333	24.916842685563147	25.388015848927264	24.037780572056253
20	25.788574979920426	24.17477592433149	25.415373624626543	24.621275471121546
21	26.658036062710217	24.26892796749277	24.439165598164863	24.633870371632156
22	25.709109116342933	24.559461928776795	24.888478235060315	24.842950719819953
23	24.284488712559735	24.192917308027827	25.616248710825353	25.90634526858708
24	24.83549201669134	24.94389075059413	25.39451977296143	24.8260974597531
25	24.966034993248293	24.378512642415583	25.47643922844719	25.179013135888933
26	24.578638635786447	25.12414235954105	25.788678216809856	24.508540787862646
27	25.330049016976307	24.774736642151296	24.917100606621215	24.97811373425118
28	24.56872789440105	24.524748979503347	25.665103660160675	25.24141946593492
29	24.6677320713656	24.748566555790248	25.558666427157085	25.025034945687075
30	24.565424313939253	24.84117004561006	25.84380671576613	24.74959892468456
31	25.1447897374273	24.52381984749847	24.979817188116197	25.351573226958042
32	24.57130881663683	24.940174222574605	25.017601889648024	25.470915071140542
33	24.33045715359378	24.58029042601735	25.548858922661115	25.540393497727752
34	24.890207568089888	24.62746968448742	25.791672086603363	24.69065066081933
35	25.631035486648372	24.68734708035753	25.411450622828152	24.270166810165943
36	24.66793854514446	25.155629610817577	25.219017060928344	24.95741478310962
37	25.34795993582795	24.850358128769436	24.97826863477473	24.823413300627887
38	24.7378299192894	24.902595994821638	25.361277494564575	24.998296591324383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	116.5
2	106.0
3	106.0
4	387.5
5	669.0
6	669.0
7	747.5
8	826.0
9	811.5
10	797.0
11	797.0
12	1064.0
13	1331.0
14	1654.5
15	1978.0
16	1978.0
17	3045.5
18	4113.0
19	4113.0
20	4867.0
21	5621.0
22	6035.5
23	6450.0
24	6450.0
25	7619.5
26	8789.0
27	8789.0
28	11581.5
29	14374.0
30	18157.0
31	21940.0
32	21940.0
33	27937.5
34	33935.0
35	33935.0
36	38121.0
37	42307.0
38	47998.5
39	53690.0
40	53690.0
41	57510.0
42	61330.0
43	68345.0
44	75360.0
45	75360.0
46	79803.0
47	84246.0
48	84246.0
49	88145.5
50	92045.0
51	91662.0
52	91279.0
53	91279.0
54	87755.0
55	84231.0
56	84231.0
57	81042.0
58	77853.0
59	70482.5
60	63112.0
61	63112.0
62	57934.5
63	52757.0
64	43411.0
65	34065.0
66	34065.0
67	28225.0
68	22385.0
69	22385.0
70	17989.5
71	13594.0
72	10705.0
73	7816.0
74	7816.0
75	5855.5
76	3895.0
77	3895.0
78	3626.5
79	3358.0
80	2635.0
81	1912.0
82	1912.0
83	1729.0
84	1546.0
85	1546.0
86	990.0
87	434.0
88	359.0
89	284.0
90	284.0
91	166.0
92	48.0
93	38.0
94	28.0
95	28.0
96	16.5
97	5.0
98	5.0
99	7.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009394556938241629
2	0.0
3	0.0
4	4.129475577249067E-4
5	1.0323688943122668E-4
6	2.0647377886245336E-4
7	3.0971066829368004E-4
8	0.0018582640097620801
9	0.004542423134973974
10	0.0011356057837434935
11	0.005161844471561334
12	2.0647377886245336E-4
13	2.0647377886245336E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0323688943122668E-4
23	3.0971066829368004E-4
24	0.0
25	2.0647377886245336E-4
26	0.0
27	2.0647377886245336E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	968646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.46895724523041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.21186580025987	51.35689529727054
2	11.55615033283084	14.438013221221299
3	2.934313355124497	5.499104865761424
4	1.1508374793772582	2.8756646918170676
5	0.5901076029864323	1.843170331052242
6	0.3356227148516219	1.2579600614756474
7	0.22563155325600595	0.9866477497467055
8	0.16206733443358484	0.8099341908464057
9	0.11684336918943608	0.6569175090855199
>10	0.5812820081511259	6.6956164696069225
>50	0.06489941631367399	2.8818102410257262
>100	0.06505485129836323	7.9500862149176
>500	0.004825039871571179	2.1611470517114153
>1k	4.991420556797771E-4	0.5870321044614089
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2622	0.27068712408867635	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1926	0.19883424904454258	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1100	0.11356057837434935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.0647377886245336E-4
13	0.0	0.0	0.0	0.0	2.0647377886245336E-4
14	0.0	0.0	0.0	0.0	2.0647377886245336E-4
15	0.0	0.0	0.0	0.0	2.0647377886245336E-4
16	0.0	0.0	0.0	2.0647377886245336E-4	2.0647377886245336E-4
17	0.0	0.0	0.0	3.0971066829368004E-4	2.0647377886245336E-4
18	0.0	0.0	0.0	3.0971066829368004E-4	3.0971066829368004E-4
19	0.0	0.0	0.0	4.129475577249067E-4	3.0971066829368004E-4
20	0.0	0.0	0.0	5.161844471561335E-4	4.129475577249067E-4
21	0.0	0.0	0.0	5.161844471561335E-4	4.129475577249067E-4
22	0.0	0.0	0.0	8.258951154498134E-4	4.129475577249067E-4
23	0.0	0.0	0.0	0.0017550271203308535	4.129475577249067E-4
24	0.0	0.0	0.0	0.0026841591252118936	4.129475577249067E-4
25	0.0	0.0	0.0	0.003200343572368027	4.129475577249067E-4
26	0.0	0.0	0.0	0.004026238687817841	4.129475577249067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGGT	75	6.394475E-8	21.334986	4
CGCGTAA	65	9.433408E-6	19.6918	10
GCGTAAC	75	1.5084388E-6	19.199505	11
ATTATAC	85	2.3930806E-7	18.824987	3
GAATAGA	70	1.785597E-5	18.28713	1
CGAACGA	80	2.7959504E-6	17.999537	16
GTGTACG	45	0.008845349	17.779156	1
GTATATA	90	4.3614273E-7	17.779156	1
GCGTTCG	45	0.008850612	17.77732	13
CCTTAAC	65	2.09956E-4	17.231215	2
TATACTA	75	3.2306954E-5	17.067987	5
TCTAGCG	190	0.0	16.841671	28
TTATACT	145	6.91216E-11	16.553007	4
CGTTTAT	185	0.0	16.432009	31
TTTATAC	90	8.571704E-6	16.00124	3
TAGTACT	100	1.3127847E-6	16.00124	4
CCACGCA	80	5.618154E-5	16.000414	9
TCGCGTA	80	5.618154E-5	16.000414	9
CGCATCG	190	0.0	15.999587	13
TAACGAA	105	2.1845535E-6	15.237702	13
>>END_MODULE