FastQCFastQC Report
Thu 2 Feb 2017
SRR4063033_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063033_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857176
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT37530.2020810090158391No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC33720.18156599051463082No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28680.154428013284686No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT28180.15173575363885813No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28080.15119730170969256No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27370.147374293012617No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG26950.1451127949101216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26360.14193592852804474No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG25300.13622833807888968No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24980.13450529190555982No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC24310.1308976639801505No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC24000.12922846299973723No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG23910.12874385626348822No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG23680.1275054168264074No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG23620.12718234566890807No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC23510.12659004854682593No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA22460.12093630329058744No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA22050.11872865038100859No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20070.10806730218353025No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC19970.10752885025436469No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC19750.10634425601020045No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT19730.10623656562436731No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT19220.1034904607856229No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG18620.10025974921062948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5900.018.9811528
GTATCAA24450.017.8117181
CTAGCGG6350.017.63697829
ATCCGGT550.00136648417.4518577
TAGCGGC6800.016.93993630
GTAATAC1354.456524E-1016.5895923
GTATTAG3950.016.2135721
AAGACGG6850.016.1121375
GTATTAC1900.016.01091
ACCGTCG5550.015.8538418
CAAGACG6900.015.763994
ATCGTTT5900.015.728085529
CATCGTT6050.015.60174428
ATACCGT6100.015.4709436
CTATTCC7700.015.37264
AGCGGCG7400.015.35022631
GTTATTC3550.015.3211713
CCTATTC7750.015.2746553
CGTTTAT6100.015.21200131
TCGTTTA6150.015.08832630
Produced by FastQC (version 0.11.5)