##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063033_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1857176
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350455207260918	32.0	32.0	32.0	32.0	32.0
2	31.04471897116913	32.0	32.0	32.0	32.0	32.0
3	31.084680181092153	32.0	32.0	32.0	32.0	32.0
4	31.13857060397076	32.0	32.0	32.0	32.0	32.0
5	31.073839528402264	32.0	32.0	32.0	32.0	32.0
6	34.71057024213106	36.0	36.0	36.0	32.0	36.0
7	34.66585127096193	36.0	36.0	36.0	32.0	36.0
8	34.6599261459334	36.0	36.0	36.0	32.0	36.0
9	34.775897383985146	36.0	36.0	36.0	32.0	36.0
10	34.535708516586475	36.0	36.0	36.0	32.0	36.0
11	34.73201678246973	36.0	36.0	36.0	32.0	36.0
12	34.588113350592515	36.0	36.0	36.0	32.0	36.0
13	34.67222869561097	36.0	36.0	36.0	32.0	36.0
14	34.56642612224151	36.0	36.0	36.0	32.0	36.0
15	34.52952493463194	36.0	36.0	36.0	32.0	36.0
16	34.542146786303505	36.0	36.0	36.0	32.0	36.0
17	34.46564838227503	36.0	36.0	36.0	32.0	36.0
18	34.48933165192744	36.0	36.0	36.0	32.0	36.0
19	34.4799022817439	36.0	36.0	36.0	32.0	36.0
20	34.46721635429275	36.0	36.0	36.0	32.0	36.0
21	34.44506713418653	36.0	36.0	36.0	32.0	36.0
22	34.42310152618815	36.0	36.0	36.0	32.0	36.0
23	34.37779671932009	36.0	36.0	36.0	32.0	36.0
24	34.360648102280024	36.0	36.0	36.0	32.0	36.0
25	34.34563713939874	36.0	36.0	36.0	32.0	36.0
26	34.28905014925888	36.0	36.0	36.0	32.0	36.0
27	34.291323493303814	36.0	36.0	36.0	32.0	36.0
28	34.288778769486576	36.0	36.0	36.0	32.0	36.0
29	34.26185455767251	36.0	36.0	36.0	32.0	36.0
30	34.23454966034452	36.0	36.0	36.0	32.0	36.0
31	34.251791429568335	36.0	36.0	36.0	32.0	36.0
32	34.2131537344872	36.0	36.0	36.0	32.0	36.0
33	34.168193536853806	36.0	36.0	36.0	32.0	36.0
34	34.19309909238543	36.0	36.0	36.0	32.0	36.0
35	34.15303288433622	36.0	36.0	36.0	32.0	36.0
36	34.11538540235282	36.0	36.0	36.0	32.0	36.0
37	34.126777968270105	36.0	36.0	36.0	32.0	36.0
38	33.519148965956916	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	2.0
10	19.0
11	13.0
12	10.0
13	6.0
14	149.0
15	393.0
16	518.0
17	630.0
18	764.0
19	1024.0
20	1278.0
21	1885.0
22	2625.0
23	4112.0
24	6134.0
25	9328.0
26	13814.0
27	20493.0
28	29148.0
29	40596.0
30	56245.0
31	78950.0
32	111250.0
33	175720.0
34	412165.0
35	889901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.994801951774924	16.694080144393823	11.075368464634996	26.23574943919626
2	17.46443679652094	18.780937993634073	36.37354455394336	27.38108065590163
3	19.352486604378146	22.47227981367296	27.778830878591236	30.396402703357655
4	12.941434300705174	14.366484162642532	34.952841161190825	37.73924037546146
5	15.458565743272967	35.59564520445344	32.97730795925631	15.968481093017283
6	36.08327302645748	34.357204087083126	15.971576077123878	13.587946809335506
7	31.7961787143491	29.281985121496295	20.144832799906958	18.777003364247655
8	29.108181686995067	32.02428208049658	18.773490016002825	20.094046216505525
9	27.340880638604332	13.91699759042632	18.1373860837024	40.60473568726695
10	16.475802995091463	25.54006844815666	30.412099687051064	27.572028869700816
11	38.989404693122715	20.488190731677637	21.229975290001903	19.29242928519775
12	25.31920117858897	23.01285420130782	27.525070861800106	24.142873758303107
13	29.561936089112056	18.171218882243398	25.100032419659023	27.166812608985524
14	24.041010099051917	19.121801437367463	23.883552341780266	32.953636121800365
15	25.759742340639246	26.514947097427445	21.361005862504477	26.364304699428832
16	26.99841462337612	24.991060716047414	22.604487305139763	25.406037355436702
17	24.95672639339936	25.391885996800927	24.11737460886799	25.534013000931715
18	26.286929714739575	23.766353001359665	25.016652531534806	24.93006475236595
19	26.12239644895535	24.278216141787617	24.41542440594691	25.183963003310122
20	26.447788294919857	23.421629940433174	23.836745331634603	26.293836433012363
21	27.83241907508692	23.46002635346927	23.51048242357144	25.197072147872362
22	26.74512152173843	23.228815412103387	24.2709611132591	25.755101952899086
23	24.893820163910327	23.057408463476666	25.023546156120304	27.025225216492704
24	25.486024048631485	24.27502983460642	24.29651721359855	25.942428903163545
25	25.58410308994089	23.689080083409447	24.371197890637117	26.355618936012547
26	25.494815018270632	24.852259661084496	24.755211236505716	24.897714084139153
27	26.433103697843997	23.628312531872627	23.956476060055973	25.982107710227403
28	25.230039050636595	23.734946765775874	25.119058132246607	25.915956051340928
29	25.00294834658339	24.141169526966735	25.055399297674224	25.800482828775646
30	24.912431850306252	24.39281146934105	25.232173386282557	25.462583294070136
31	25.804789264809617	24.2847065538725	23.73216949280318	26.178334688514703
32	25.660198540161705	23.92083163934294	23.495588445145675	26.92338137534968
33	24.94523472064861	23.65116940295614	24.522621237512222	26.880974638883025
34	26.345207078795816	23.6788649628472	24.40425735866696	25.571670599690027
35	26.786665395699067	23.41536998351512	24.821943363314166	24.976021257471647
36	25.06681860505694	24.497749735714443	24.08006277152211	26.35536888770651
37	26.519040987746862	24.122641214780447	24.104547456803846	25.253770340668847
38	25.247205659277018	23.719788891778766	24.662086301066687	26.37091914787753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	69.0
2	95.0
3	95.0
4	287.0
5	479.0
6	479.0
7	605.0
8	731.0
9	690.0
10	649.0
11	649.0
12	897.5
13	1146.0
14	1629.0
15	2112.0
16	2112.0
17	3212.0
18	4312.0
19	4312.0
20	5516.5
21	6721.0
22	7421.0
23	8121.0
24	8121.0
25	9925.5
26	11730.0
27	11730.0
28	15103.0
29	18476.0
30	23847.0
31	29218.0
32	29218.0
33	39449.5
34	49681.0
35	49681.0
36	57505.0
37	65329.0
38	77207.0
39	89085.0
40	89085.0
41	96554.5
42	104024.0
43	122424.5
44	140825.0
45	140825.0
46	144132.5
47	147440.0
48	147440.0
49	162029.5
50	176619.0
51	183802.5
52	190986.0
53	190986.0
54	182502.0
55	174018.0
56	174018.0
57	170561.0
58	167104.0
59	150606.0
60	134108.0
61	134108.0
62	127588.0
63	121068.0
64	100322.5
65	79577.0
66	79577.0
67	66652.5
68	53728.0
69	53728.0
70	42609.0
71	31490.0
72	24309.0
73	17128.0
74	17128.0
75	12737.0
76	8346.0
77	8346.0
78	8417.5
79	8489.0
80	6578.0
81	4667.0
82	4667.0
83	4853.0
84	5039.0
85	5039.0
86	3298.0
87	1557.0
88	1355.5
89	1154.0
90	1154.0
91	817.0
92	480.0
93	405.5
94	331.0
95	331.0
96	304.5
97	278.0
98	278.0
99	535.0
100	792.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1210978388693371
2	0.056214381404885695
3	0.012169013599141923
4	0.0025845692599947446
5	2.6922596458278593E-4
6	4.307615433324574E-4
7	0.0
8	2.153807716662287E-4
9	5.384519291655718E-5
10	2.153807716662287E-4
11	0.001776891366246387
12	0.0021538077166622874
13	0.014968963630802896
14	0.009153682795814721
15	0.024822633934532864
16	0.010984419354977666
17	0.020784244465791073
18	0.005438364484572276
19	0.007807552972900792
20	0.005492209677488833
21	0.006192197185404076
22	0.00764601739415112
23	0.009961360689563077
24	0.014645892473303553
25	0.016799700189965842
26	0.019976566572042713
27	0.009422908760397506
28	0.005384519291655719
29	0.010930574162061108
30	0.0027461048387444164
31	0.0057075904491550615
32	0.0065152683429034195
33	0.00861523086664915
34	0.013461298229139296
35	0.01739199731204797
36	0.015238189595385683
37	0.009799825110813407
38	0.006407577957070304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1857176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.44021621527749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70702151514303	38.91292161369169
2	12.43143785101771	12.292259504422004
3	3.8220662280542306	5.668913421123338
4	1.6398471515033608	3.242975909213322
5	0.870099290343619	2.1508948521674016
6	0.5336830574317843	1.5831243449914663
7	0.3507280967551343	1.2138051055442622
8	0.262255203921972	1.0372763188387188
9	0.1885666392358774	0.8390497873329007
>10	1.0125960760469432	8.889445489270837
>50	0.0776181721551055	2.707046130805578
>100	0.0795659306971945	8.765549964035348
>500	0.015431183325241685	5.330978402245993
>1k	0.00908360436875929	7.365759156317171
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3753	0.2020810090158391	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3372	0.18156599051463082	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2868	0.154428013284686	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2818	0.15173575363885813	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2808	0.15119730170969256	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2737	0.147374293012617	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2695	0.1451127949101216	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2636	0.14193592852804474	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2530	0.13622833807888968	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2498	0.13450529190555982	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2431	0.1308976639801505	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2400	0.12922846299973723	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2391	0.12874385626348822	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2368	0.1275054168264074	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2362	0.12718234566890807	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2351	0.12659004854682593	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2246	0.12093630329058744	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2205	0.11872865038100859	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2007	0.10806730218353025	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1997	0.10752885025436469	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1975	0.10634425601020045	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1973	0.10623656562436731	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1922	0.1034904607856229	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1862	0.10025974921062948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.3845192916557183E-5
2	0.0	0.0	0.0	0.0	5.3845192916557183E-5
3	0.0	0.0	0.0	0.0	5.3845192916557183E-5
4	0.0	0.0	0.0	0.0	5.3845192916557183E-5
5	0.0	0.0	0.0	0.0	5.3845192916557183E-5
6	0.0	0.0	0.0	0.0	5.3845192916557183E-5
7	0.0	0.0	0.0	0.0	1.0769038583311437E-4
8	0.0	0.0	0.0	0.0	1.0769038583311437E-4
9	0.0	0.0	0.0	0.0	1.0769038583311437E-4
10	0.0	0.0	0.0	0.0	1.6153557874967154E-4
11	0.0	0.0	0.0	0.0	2.1538077166622873E-4
12	0.0	0.0	0.0	0.0	3.7691635041590027E-4
13	0.0	0.0	0.0	0.0	3.7691635041590027E-4
14	0.0	0.0	0.0	0.0	3.7691635041590027E-4
15	0.0	0.0	0.0	5.3845192916557183E-5	5.384519291655719E-4
16	0.0	0.0	0.0	1.0769038583311437E-4	5.384519291655719E-4
17	0.0	0.0	0.0	1.0769038583311437E-4	5.92297122082129E-4
18	0.0	0.0	0.0	1.6153557874967154E-4	6.461423149986861E-4
19	0.0	0.0	0.0	1.6153557874967154E-4	6.461423149986861E-4
20	0.0	0.0	0.0	2.1538077166622873E-4	6.999875079152434E-4
21	0.0	0.0	0.0	2.6922596458278593E-4	7.538327008318005E-4
22	0.0	0.0	0.0	3.7691635041590027E-4	8.076778937483577E-4
23	0.0	0.0	0.0	5.92297122082129E-4	8.615230866649149E-4
24	0.0	0.0	0.0	8.076778937483577E-4	8.615230866649149E-4
25	0.0	0.0	0.0	9.692134724980292E-4	8.615230866649149E-4
26	0.0	0.0	0.0	0.001184594244164258	9.153682795814721E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	590	0.0	18.98115	28
GTATCAA	2445	0.0	17.811718	1
CTAGCGG	635	0.0	17.636978	29
ATCCGGT	55	0.001366484	17.451857	7
TAGCGGC	680	0.0	16.939936	30
GTAATAC	135	4.456524E-10	16.589592	3
GTATTAG	395	0.0	16.213572	1
AAGACGG	685	0.0	16.112137	5
GTATTAC	190	0.0	16.0109	1
ACCGTCG	555	0.0	15.853841	8
CAAGACG	690	0.0	15.76399	4
ATCGTTT	590	0.0	15.7280855	29
CATCGTT	605	0.0	15.601744	28
ATACCGT	610	0.0	15.470943	6
CTATTCC	770	0.0	15.3726	4
AGCGGCG	740	0.0	15.350226	31
GTTATTC	355	0.0	15.321171	3
CCTATTC	775	0.0	15.274655	3
CGTTTAT	610	0.0	15.212001	31
TCGTTTA	615	0.0	15.088326	30
>>END_MODULE