FastQCFastQC Report
Thu 2 Feb 2017
SRR4063033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857176
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT36610.19712725126751585No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34840.1875966521212852No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC30610.16482013551758154No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG29840.16067405566300663No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29150.1569587373517642No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC28830.15523569117843436No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC28220.15195113441052438No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT28200.15184344402469124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27440.1477512093630329No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG26980.14527433048887128No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24890.13402068516931082No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23190.1248670023734961No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG22630.1218516715701689No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC22520.12125937444808677No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22440.12082861290475432No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC22360.12039785136142185No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22210.11959017346767349No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC20130.1083903733410296No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG20050.10795961179769714No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19690.1060211848527011No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA19630.10569811369520174No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19620.10564426850228519No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT19180.10327508001395669No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT19060.10262893769895798No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT18950.10203664057687586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG302.247298E-531.99943516
TCTAGCG6600.021.33295628
CTAGCGG6800.020.70551729
TAGCGGC7050.019.74433330
ACGAACG3700.017.72941615
GTATCAA21850.017.5036621
ACCGTCG6800.017.4114578
ATACCGT6850.017.2843656
CGATAAC4000.017.19969610
TACCGTC6650.017.0824057
ATAACGA4150.016.96355412
TAACGAA3900.016.82021513
CCGATAA4100.016.7801919
AGCGGCG8200.016.78019131
CGAACGA3950.016.60730216
GATAACG4150.016.5780211
CGTCGTA7100.016.45041510
CGGTCCA8800.015.99971910
ACGTTGC703.698426E-415.99971815
TCTAGAT4850.015.8347722
Produced by FastQC (version 0.11.5)