##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1857176
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23781806355456	32.0	32.0	32.0	32.0	32.0
2	31.24780473148479	32.0	32.0	32.0	32.0	32.0
3	31.347674103046778	32.0	32.0	32.0	32.0	32.0
4	31.459001193209474	32.0	32.0	32.0	32.0	32.0
5	31.35858313913167	32.0	32.0	32.0	32.0	32.0
6	34.91429029881928	36.0	36.0	36.0	36.0	36.0
7	34.942543948446456	36.0	36.0	36.0	36.0	36.0
8	34.87079199817357	36.0	36.0	36.0	32.0	36.0
9	35.00869384484831	36.0	36.0	36.0	36.0	36.0
10	34.82937912184952	36.0	36.0	36.0	32.0	36.0
11	35.01826859705273	36.0	36.0	36.0	36.0	36.0
12	34.905440302911515	36.0	36.0	36.0	32.0	36.0
13	34.97360777869195	36.0	36.0	36.0	36.0	36.0
14	34.91007852782935	36.0	36.0	36.0	32.0	36.0
15	34.88117227446403	36.0	36.0	36.0	32.0	36.0
16	34.891547166235185	36.0	36.0	36.0	32.0	36.0
17	34.844587696588796	36.0	36.0	36.0	32.0	36.0
18	34.85744054413798	36.0	36.0	36.0	32.0	36.0
19	34.856868169737275	36.0	36.0	36.0	32.0	36.0
20	34.84596936423904	36.0	36.0	36.0	32.0	36.0
21	34.844074551900306	36.0	36.0	36.0	32.0	36.0
22	34.82671916931944	36.0	36.0	36.0	32.0	36.0
23	34.76275108013457	36.0	36.0	36.0	32.0	36.0
24	34.740465093238335	36.0	36.0	36.0	32.0	36.0
25	34.71686474518301	36.0	36.0	36.0	32.0	36.0
26	34.650451545787796	36.0	36.0	36.0	32.0	36.0
27	34.64627854333676	36.0	36.0	36.0	32.0	36.0
28	34.61974470917134	36.0	36.0	36.0	32.0	36.0
29	34.57827637229859	36.0	36.0	36.0	32.0	36.0
30	34.559223789236995	36.0	36.0	36.0	32.0	36.0
31	34.566909113621975	36.0	36.0	36.0	32.0	36.0
32	34.539081917922694	36.0	36.0	36.0	32.0	36.0
33	34.50694010691502	36.0	36.0	36.0	32.0	36.0
34	34.50168427763443	36.0	36.0	36.0	32.0	36.0
35	34.47427168992061	36.0	36.0	36.0	32.0	36.0
36	34.44909367771283	36.0	36.0	36.0	32.0	36.0
37	34.45026588756262	36.0	36.0	36.0	32.0	36.0
38	33.99256074814665	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	24.0
22	105.0
23	396.0
24	1055.0
25	2662.0
26	5678.0
27	11378.0
28	20135.0
29	32668.0
30	49813.0
31	73839.0
32	108851.0
33	179135.0
34	443999.0
35	927431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2513556739059	17.973301166929634	11.837577611321425	26.93776554784304
2	16.42057618664036	19.78800070644893	35.733231530021925	28.05819157688878
3	18.26967395658785	23.208516586473227	28.10562919184827	30.416180265090652
4	12.27263157497982	15.174569089686313	35.06582316240867	37.486976172925196
5	14.896980629181417	36.15340503722051	32.62083540861793	16.328778924980146
6	36.199055552265	34.805806684363844	15.560557191856429	13.434580571514724
7	31.555266704609764	29.63949930189369	20.040254861302188	18.76497913219436
8	29.012546772414012	32.264013685625834	18.550064588873912	20.17337495308624
9	27.299440419061078	13.692824957568343	17.941590777899354	41.066143845471224
10	16.285826745790764	25.800803051098796	30.38827418320485	27.525096019905586
11	39.24456470524885	20.41043038111008	21.131402878182087	19.213602035458987
12	25.149097981442715	23.120363908919387	27.538165637142143	24.19237247249576
13	29.58383521621303	18.212089019170534	25.112253947262857	27.09182181735358
14	24.011348412859093	19.094151550526174	23.814113471205744	33.080386565408986
15	25.83341589596247	26.7047388077382	21.159976221962808	26.30186907433652
16	27.040531990792466	25.057035551307766	22.39966615962416	25.50276629827561
17	24.942224108000534	25.498175724863987	23.93047293309842	25.629127234037053
18	26.35614502879641	23.8116904375245	24.97205434487631	24.860110188802782
19	26.275054168264074	24.283589708245206	24.298127910332674	25.143228213158043
20	26.47061990893701	23.415766734009054	23.767321998561254	26.346291358492678
21	27.968069783407596	23.464670803774553	23.490678045956898	25.076581366860957
22	26.674208583354513	23.312976260731347	24.19452975916122	25.81828539675292
23	24.86451181446209	23.0592949606741	25.044139668194614	27.032053556669194
24	25.52746750981059	24.229475289364068	24.27163607541773	25.971421125407606
25	25.684575427275757	23.676954166359298	24.355620074166488	26.282850332198453
26	25.51667210503701	24.7867997290507	24.700108875097328	24.99641929081497
27	26.419656962675546	23.625196131326412	23.897114648385127	26.05803225761291
28	25.29587933507648	23.766568165860424	25.081898538426085	25.85565396063701
29	25.167404704777574	23.984425816400815	25.024014956040787	25.824154522780823
30	24.998923096141667	24.280843603406463	25.28263341761901	25.437599882832863
31	25.95489065118223	24.119415715042624	23.68655420918642	26.239139424588732
32	25.812900877461264	23.83651307145903	23.52938009106299	26.821205960016712
33	25.054060573688226	23.529487781448825	24.493155199076448	26.923296445786505
34	26.34063761323644	23.70184624397472	24.39892611147247	25.558590031316363
35	26.816359892654223	23.427935747608196	24.721566507428484	25.034137852309097
36	25.218557638048306	24.430910156064908	24.107623617793898	26.242908588092888
37	26.71017717222277	24.053670734491508	23.980548962510824	25.255603130774894
38	25.38799562560946	23.522310522498444	24.62753873764049	26.46215511425161
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	33.5
2	53.0
3	53.0
4	197.0
5	341.0
6	341.0
7	462.0
8	583.0
9	597.5
10	612.0
11	612.0
12	831.5
13	1051.0
14	1515.5
15	1980.0
16	1980.0
17	3140.5
18	4301.0
19	4301.0
20	5373.0
21	6445.0
22	7277.5
23	8110.0
24	8110.0
25	9943.0
26	11776.0
27	11776.0
28	15146.0
29	18516.0
30	23625.0
31	28734.0
32	28734.0
33	39797.0
34	50860.0
35	50860.0
36	58073.5
37	65287.0
38	77575.0
39	89863.0
40	89863.0
41	97070.5
42	104278.0
43	123243.0
44	142208.0
45	142208.0
46	144508.5
47	146809.0
48	146809.0
49	161866.0
50	176923.0
51	183204.0
52	189485.0
53	189485.0
54	182571.5
55	175658.0
56	175658.0
57	171475.0
58	167292.0
59	150832.5
60	134373.0
61	134373.0
62	128169.5
63	121966.0
64	100950.0
65	79934.0
66	79934.0
67	66898.5
68	53863.0
69	53863.0
70	42827.5
71	31792.0
72	24408.0
73	17024.0
74	17024.0
75	12562.5
76	8101.0
77	8101.0
78	8192.5
79	8284.0
80	6406.0
81	4528.0
82	4528.0
83	4269.5
84	4011.0
85	4011.0
86	2567.5
87	1124.0
88	942.5
89	761.0
90	761.0
91	451.5
92	142.0
93	94.0
94	46.0
95	46.0
96	33.5
97	21.0
98	21.0
99	24.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008938302024148491
2	0.0
3	0.0
4	1.6153557874967154E-4
5	5.384519291655718E-5
6	3.230711574993431E-4
7	4.8460673624901466E-4
8	0.002423033681245073
9	0.0038768538899921173
10	4.8460673624901466E-4
11	0.005438364484572276
12	4.307615433324574E-4
13	1.6153557874967154E-4
14	0.0
15	0.0
16	5.384519291655718E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	5.384519291655718E-5
22	0.0
23	1.6153557874967154E-4
24	0.0
25	1.6153557874967154E-4
26	1.0769038583311435E-4
27	1.0769038583311435E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.6153557874967154E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1857176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.76210110823138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2698981967702	36.905726902968844
2	13.21520000699594	12.623714377992801
3	4.125400693416062	5.911134150927175
4	1.7517689526200835	3.346726633332041
5	0.9367168529688146	2.2369782520640418
6	0.5762093692288349	1.6512582091570704
7	0.3722648211468892	1.2446105018658777
8	0.2807056526917789	1.0725673412413461
9	0.19529494792363974	0.8394927343790097
>10	1.0855153500934713	9.140448707897677
>50	0.07952722327329453	2.66893075288936
>100	0.08467534868256185	8.876051124425825
>500	0.017275562697707306	5.775928773935391
>1k	0.009547021490838248	7.706431536923533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3661	0.19712725126751585	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3484	0.1875966521212852	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3061	0.16482013551758154	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2984	0.16067405566300663	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2915	0.1569587373517642	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2883	0.15523569117843436	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2822	0.15195113441052438	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2820	0.15184344402469124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2744	0.1477512093630329	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2698	0.14527433048887128	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2489	0.13402068516931082	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2319	0.1248670023734961	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2263	0.1218516715701689	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2252	0.12125937444808677	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2244	0.12082861290475432	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2236	0.12039785136142185	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2221	0.11959017346767349	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2013	0.1083903733410296	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2005	0.10795961179769714	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1969	0.1060211848527011	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1963	0.10569811369520174	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1962	0.10564426850228519	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1918	0.10327508001395669	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1906	0.10262893769895798	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1895	0.10203664057687586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6922596458278593E-4
2	0.0	0.0	0.0	0.0	2.6922596458278593E-4
3	0.0	0.0	0.0	0.0	2.6922596458278593E-4
4	0.0	0.0	0.0	0.0	3.230711574993431E-4
5	0.0	0.0	0.0	0.0	3.230711574993431E-4
6	0.0	0.0	0.0	0.0	3.7691635041590027E-4
7	0.0	0.0	0.0	0.0	3.7691635041590027E-4
8	0.0	0.0	0.0	0.0	3.7691635041590027E-4
9	0.0	0.0	0.0	0.0	3.7691635041590027E-4
10	0.0	0.0	0.0	0.0	3.7691635041590027E-4
11	0.0	0.0	0.0	0.0	3.7691635041590027E-4
12	0.0	0.0	0.0	0.0	5.384519291655719E-4
13	0.0	0.0	0.0	0.0	6.461423149986861E-4
14	0.0	0.0	0.0	0.0	6.999875079152434E-4
15	0.0	0.0	0.0	1.0769038583311437E-4	7.538327008318005E-4
16	0.0	0.0	0.0	1.6153557874967154E-4	8.076778937483577E-4
17	0.0	0.0	0.0	1.6153557874967154E-4	8.076778937483577E-4
18	0.0	0.0	0.0	2.1538077166622873E-4	9.153682795814721E-4
19	0.0	0.0	0.0	2.1538077166622873E-4	9.153682795814721E-4
20	0.0	0.0	0.0	2.6922596458278593E-4	9.692134724980292E-4
21	0.0	0.0	0.0	3.230711574993431E-4	0.001184594244164258
22	0.0	0.0	0.0	4.3076154333245747E-4	0.001184594244164258
23	0.0	0.0	0.0	5.92297122082129E-4	0.001184594244164258
24	0.0	0.0	0.0	7.538327008318005E-4	0.001184594244164258
25	0.0	0.0	0.0	9.153682795814721E-4	0.001184594244164258
26	0.0	0.0	0.0	0.0011307490512477009	0.001184594244164258
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCG	30	2.247298E-5	31.999435	16
TCTAGCG	660	0.0	21.332956	28
CTAGCGG	680	0.0	20.705517	29
TAGCGGC	705	0.0	19.744333	30
ACGAACG	370	0.0	17.729416	15
GTATCAA	2185	0.0	17.503662	1
ACCGTCG	680	0.0	17.411457	8
ATACCGT	685	0.0	17.284365	6
CGATAAC	400	0.0	17.199696	10
TACCGTC	665	0.0	17.082405	7
ATAACGA	415	0.0	16.963554	12
TAACGAA	390	0.0	16.820215	13
CCGATAA	410	0.0	16.780191	9
AGCGGCG	820	0.0	16.780191	31
CGAACGA	395	0.0	16.607302	16
GATAACG	415	0.0	16.57802	11
CGTCGTA	710	0.0	16.450415	10
CGGTCCA	880	0.0	15.999719	10
ACGTTGC	70	3.698426E-4	15.999718	15
TCTAGAT	485	0.0	15.834772	2
>>END_MODULE