FastQCFastQC Report
Thu 2 Feb 2017
SRR4063032_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063032_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525635
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35410.23210007636164615No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25320.16596368069689016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24020.15744263863899294No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23310.15278883874583368No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19640.12873328155161623No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19030.12473494643214138No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18750.12289964506582506No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18440.1208677042674034No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18420.12073661131266653No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18280.11981896062950836No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16900.11077354675266364No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16670.10926597777318953No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15900.10421889901581964No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15870.10402225958371432No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15820.10369452719687212No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15790.1034978877647668No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15420.10107266810213453No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15280.10015501741897637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT4050.019.35774828
ATCGTTT4200.018.66639929
ATCGGCT450.00885229517.77752327
ACTAGGT450.00885563817.77635818
TCTAGCG4900.017.632428
CGTTTAT4300.017.48754931
CTAGCGG5050.017.10866529
CAAGACG5750.016.9714454
AAGACGG5850.016.6813345
TACCGTC4150.016.5769887
ACCGTCG4000.016.398698
CGCAAGA6000.016.2701662
CGTCGTA4050.016.19729810
GTATAGG1203.0751835E-816.015521
GCGCAAG6450.015.8913681
TAGCGGC5450.015.85246530
GTATCAA28000.015.7295281
ATACCGT4500.015.6421696
GCATCGT5250.015.54263527
GACGGAC6400.015.498767
Produced by FastQC (version 0.11.5)