FastQCFastQC Report
Thu 2 Feb 2017
SRR4063032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525635
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39710.2602850616300753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26400.17304270025268167No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25240.16543930887794261No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25070.16432501876267913No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20300.13305934905793326No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20170.13220724485214352No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19600.12847109564214246No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19500.12781563086845804No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19410.12722571257214207No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18290.11988450710687681No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18080.11850803108213957No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17450.11437860300792785No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16180.10605420038213598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16080.10539873560845156No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15560.10199031878529267No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15450.10126930753423984No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15440.1012037610568714No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15320.10041720332845012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4350.018.75840828
CTAGCGG4300.018.6044429
TAGCGGC4700.017.36150630
ACGGCGT753.2342155E-517.0670348
TCCTATA957.697363E-716.8419152
GTATTAG2900.016.0019171
GGTGCGT600.00244204716.0003458
CGCTTCG4450.015.82004532
AAACGCT4350.015.81591329
TAAACGC4250.015.81158728
CGAAAAC3950.015.79729223
CCGAAAA4100.015.6095822
GTATAAA1357.903509E-915.4092531
TTCCGAA4250.015.05865520
AGCGGCG5500.014.83619731
GTATCAA26700.014.8032711
TCCGAAA4250.014.68218821
ACGCTTC5050.014.57409431
AATAGCG3750.014.5065035
CGGTCCA5750.014.46987710
Produced by FastQC (version 0.11.5)