##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063032_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1525635
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22915310673916	32.0	32.0	32.0	32.0	32.0
2	31.302597279165724	32.0	32.0	32.0	32.0	32.0
3	31.382921865321652	32.0	32.0	32.0	32.0	32.0
4	31.475943459608622	32.0	32.0	32.0	32.0	32.0
5	31.401271601660948	32.0	32.0	32.0	32.0	32.0
6	34.93469473366827	36.0	36.0	36.0	36.0	36.0
7	34.961468503278965	36.0	36.0	36.0	36.0	36.0
8	34.887834901532806	36.0	36.0	36.0	32.0	36.0
9	35.012988034490554	36.0	36.0	36.0	36.0	36.0
10	34.857503924595335	36.0	36.0	36.0	32.0	36.0
11	35.02927633411662	36.0	36.0	36.0	36.0	36.0
12	34.925502495682125	36.0	36.0	36.0	32.0	36.0
13	34.98393062560835	36.0	36.0	36.0	36.0	36.0
14	34.92889976960413	36.0	36.0	36.0	32.0	36.0
15	34.902344269763084	36.0	36.0	36.0	32.0	36.0
16	34.91274846211577	36.0	36.0	36.0	32.0	36.0
17	34.881206186276536	36.0	36.0	36.0	32.0	36.0
18	34.873743719828134	36.0	36.0	36.0	32.0	36.0
19	34.86317697221157	36.0	36.0	36.0	32.0	36.0
20	34.851518220282045	36.0	36.0	36.0	32.0	36.0
21	34.85278785554868	36.0	36.0	36.0	32.0	36.0
22	34.8404926473239	36.0	36.0	36.0	32.0	36.0
23	34.781581439859465	36.0	36.0	36.0	32.0	36.0
24	34.755352361475715	36.0	36.0	36.0	32.0	36.0
25	34.740042670756765	36.0	36.0	36.0	32.0	36.0
26	34.68370809531769	36.0	36.0	36.0	32.0	36.0
27	34.6647428775559	36.0	36.0	36.0	32.0	36.0
28	34.64585631556696	36.0	36.0	36.0	32.0	36.0
29	34.605688778770805	36.0	36.0	36.0	32.0	36.0
30	34.59288034162824	36.0	36.0	36.0	32.0	36.0
31	34.58873387146991	36.0	36.0	36.0	32.0	36.0
32	34.56060132338338	36.0	36.0	36.0	32.0	36.0
33	34.531312535436065	36.0	36.0	36.0	32.0	36.0
34	34.52020896216985	36.0	36.0	36.0	32.0	36.0
35	34.48284419274597	36.0	36.0	36.0	32.0	36.0
36	34.46651525430394	36.0	36.0	36.0	32.0	36.0
37	34.46392158019447	36.0	36.0	36.0	32.0	36.0
38	34.03554323281781	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	36.0
22	87.0
23	300.0
24	917.0
25	2290.0
26	4879.0
27	9645.0
28	16814.0
29	27160.0
30	41325.0
31	59762.0
32	86214.0
33	140698.0
34	346126.0
35	789370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10267407933283	18.319406724497	11.981341162912807	26.596578033257355
2	16.121418294677298	20.14295686714057	36.353387278084206	27.382237560097927
3	18.172498664490526	23.58663769512367	28.57918178332301	29.6616818570628
4	12.188464969576522	15.78166673330576	35.606971803797904	36.42289649331981
5	14.455760687032408	36.67340921872481	33.065401007056735	15.805429087186049
6	35.10614938192868	35.56549969750261	15.964652049346077	13.363698871222635
7	30.817251410240782	30.430721552988736	20.249458255168697	18.502568781601784
8	28.505778016662187	32.75614344426164	18.889493382974678	19.848585156101496
9	27.20949622896038	13.932264183841623	18.38979951152812	40.46844007566988
10	15.94958115389153	26.314023151243426	31.079364455106777	26.65703123975826
11	38.240343375772426	21.012166687751836	21.550592274700616	19.196897661775125
12	24.75018500565996	23.723657586477444	28.098639970792377	23.427517437070218
13	29.377988925245408	18.932219565399784	25.222989554492827	26.466801954861985
14	23.55216024802787	19.64677003346148	24.697060568222412	32.10400915028824
15	25.36825649647524	27.13361977143943	21.89907808879581	25.599045643289514
16	26.302752624317087	25.69126953694691	23.14806621505144	24.857911623684565
17	24.39154843720811	25.910456957266977	24.585041638399748	25.112952967125164
18	25.392312053669457	24.58104330328027	25.73367810780429	24.29296653524598
19	25.65456350962058	24.822188793518766	25.24253835288257	24.28070934397808
20	25.87788035801486	24.037073087599587	24.720132928256103	25.36491362612945
21	27.226963793997367	24.10274830545525	24.308302597417068	24.36198530313031
22	25.98337870247956	23.97529418936271	25.043834264203774	24.997492843953953
23	24.435921923453314	23.81322875583939	25.59019841626186	26.160650904445436
24	24.842835933889823	24.835494728424557	25.113739524853585	25.207929812832035
25	25.018484142965015	24.31720100260089	25.24330900243309	25.421005852001006
26	24.8580427166393	25.3681254035205	25.341972359050498	24.431859520789704
27	25.656267718032165	24.44595201342392	24.701058903341888	25.19672136520203
28	24.631907369718185	24.550629737781318	25.74298570759061	25.074477184909888
29	24.651243580541873	24.713316094609787	25.66183916860848	24.973601156239862
30	24.456242810370764	24.945874996313012	25.890399735192233	24.707482458123994
31	25.280489763278897	24.770144890488226	24.482723587227614	25.466641759005267
32	25.011028194817243	24.639379668138183	24.544337275953946	25.805254861090628
33	24.545254926637107	24.33622721030915	25.245815676750993	25.872702186302753
34	25.417940726320516	24.483903423820244	25.31844117367522	24.779714676184014
35	26.01919856322122	24.218505736955432	25.32905970300891	24.433235996814442
36	24.598150933873438	25.153788422525704	24.85011159287772	25.39794905072314
37	25.836848263182215	24.84224601559351	24.66559825908556	24.655307462138715
38	24.660337486366306	24.42948233912241	25.391968705428308	25.518211469082974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	47.0
2	80.0
3	80.0
4	263.5
5	447.0
6	447.0
7	663.0
8	879.0
9	958.0
10	1037.0
11	1037.0
12	1413.0
13	1789.0
14	2527.0
15	3265.0
16	3265.0
17	4743.5
18	6222.0
19	6222.0
20	7744.0
21	9266.0
22	10080.5
23	10895.0
24	10895.0
25	12541.5
26	14188.0
27	14188.0
28	18052.0
29	21916.0
30	26644.5
31	31373.0
32	31373.0
33	40592.0
34	49811.0
35	49811.0
36	56120.5
37	62430.0
38	71995.0
39	81560.0
40	81560.0
41	87633.5
42	93707.0
43	107090.5
44	120474.0
45	120474.0
46	122026.5
47	123579.0
48	123579.0
49	132537.5
50	141496.0
51	144482.5
52	147469.0
53	147469.0
54	140603.0
55	133737.0
56	133737.0
57	128934.5
58	124132.0
59	111484.0
60	98836.0
61	98836.0
62	93584.0
63	88332.0
64	73363.5
65	58395.0
66	58395.0
67	49282.5
68	40170.0
69	40170.0
70	32349.5
71	24529.0
72	19018.5
73	13508.0
74	13508.0
75	10224.0
76	6940.0
77	6940.0
78	6779.0
79	6618.0
80	5161.0
81	3704.0
82	3704.0
83	3441.5
84	3179.0
85	3179.0
86	2049.5
87	920.0
88	737.0
89	554.0
90	554.0
91	332.0
92	110.0
93	77.0
94	44.0
95	44.0
96	29.5
97	15.0
98	15.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009045413876844724
2	0.0
3	0.0
4	2.6218590947376015E-4
5	6.554647736844004E-5
6	1.3109295473688007E-4
7	5.899182963159603E-4
8	0.0016386619342110007
9	0.003998335119474842
10	9.831971605266004E-4
11	0.0049815322800014425
12	3.9327886421064017E-4
13	1.9663943210532008E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.6218590947376015E-4
22	1.3109295473688007E-4
23	2.6218590947376015E-4
24	0.0
25	1.9663943210532008E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.9663943210532008E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1525635.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.16289094164598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54203568331225	43.87768639675067
2	12.985762852003246	14.326644399982655
3	3.6357924491680635	6.016824670797535
4	1.42546074845808	3.145301432351605
5	0.713973997595705	1.9692434882271446
6	0.4022230031939792	1.3312670195646479
7	0.24434124219543563	0.9434998507041187
8	0.17664751818620572	0.7795510224654473
9	0.1197423905766843	0.5944802789226252
>10	0.5960732120649916	6.010139616109053
>50	0.06435826699167356	2.522729095048602
>100	0.07613798179034206	9.297442101378874
>500	0.011952503057098366	4.518486102266207
>1k	0.005498151406265248	4.666704525430825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3971	0.2602850616300753	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2640	0.17304270025268167	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2524	0.16543930887794261	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2507	0.16432501876267913	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2030	0.13305934905793326	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2017	0.13220724485214352	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1960	0.12847109564214246	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1950	0.12781563086845804	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1941	0.12722571257214207	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1829	0.11988450710687681	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1808	0.11850803108213957	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1745	0.11437860300792785	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1618	0.10605420038213598	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1608	0.10539873560845156	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1556	0.10199031878529267	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1545	0.10126930753423984	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1544	0.1012037610568714	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1532	0.10041720332845012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3109295473688005E-4
2	0.0	0.0	0.0	6.554647736844002E-5	1.3109295473688005E-4
3	0.0	0.0	0.0	6.554647736844002E-5	1.3109295473688005E-4
4	0.0	0.0	0.0	6.554647736844002E-5	1.3109295473688005E-4
5	0.0	0.0	0.0	6.554647736844002E-5	1.3109295473688005E-4
6	0.0	0.0	0.0	6.554647736844002E-5	3.2773238684220016E-4
7	0.0	0.0	0.0	6.554647736844002E-5	3.2773238684220016E-4
8	0.0	0.0	0.0	6.554647736844002E-5	3.2773238684220016E-4
9	0.0	0.0	0.0	1.3109295473688005E-4	3.2773238684220016E-4
10	0.0	0.0	0.0	1.3109295473688005E-4	3.2773238684220016E-4
11	0.0	0.0	0.0	1.9663943210532008E-4	3.2773238684220016E-4
12	0.0	0.0	0.0	1.9663943210532008E-4	6.554647736844003E-4
13	0.0	0.0	0.0	1.9663943210532008E-4	7.210112510528403E-4
14	0.0	0.0	0.0	1.9663943210532008E-4	7.210112510528403E-4
15	0.0	0.0	0.0	1.9663943210532008E-4	9.831971605266004E-4
16	0.0	0.0	0.0	1.9663943210532008E-4	9.831971605266004E-4
17	0.0	0.0	0.0	1.9663943210532008E-4	9.831971605266004E-4
18	0.0	0.0	0.0	1.9663943210532008E-4	0.0011798365926319204
19	0.0	6.554647736844002E-5	0.0	1.9663943210532008E-4	0.0011798365926319204
20	0.0	6.554647736844002E-5	0.0	1.9663943210532008E-4	0.0014420225021056807
21	0.0	6.554647736844002E-5	0.0	2.621859094737601E-4	0.0015075689794741207
22	0.0	6.554647736844002E-5	0.0	3.2773238684220016E-4	0.0016386619342110007
23	0.0	6.554647736844002E-5	0.0	7.865577284212803E-4	0.0016386619342110007
24	0.0	6.554647736844002E-5	0.0	0.0015731154568425607	0.0016386619342110007
25	0.0	6.554647736844002E-5	0.0	0.0017042084115794407	0.0016386619342110007
26	0.0	6.554647736844002E-5	0.0	0.0021630337531585208	0.0017042084115794407
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	435	0.0	18.758408	28
CTAGCGG	430	0.0	18.60444	29
TAGCGGC	470	0.0	17.361506	30
ACGGCGT	75	3.2342155E-5	17.067034	8
TCCTATA	95	7.697363E-7	16.841915	2
GTATTAG	290	0.0	16.001917	1
GGTGCGT	60	0.002442047	16.000345	8
CGCTTCG	445	0.0	15.820045	32
AAACGCT	435	0.0	15.815913	29
TAAACGC	425	0.0	15.811587	28
CGAAAAC	395	0.0	15.797292	23
CCGAAAA	410	0.0	15.60958	22
GTATAAA	135	7.903509E-9	15.409253	1
TTCCGAA	425	0.0	15.058655	20
AGCGGCG	550	0.0	14.836197	31
GTATCAA	2670	0.0	14.803271	1
TCCGAAA	425	0.0	14.682188	21
ACGCTTC	505	0.0	14.574094	31
AATAGCG	375	0.0	14.506503	5
CGGTCCA	575	0.0	14.469877	10
>>END_MODULE