FastQCFastQC Report
Thu 2 Feb 2017
SRR4063031_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063031_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275100
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26400.9596510359869139No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18660.678298800436205No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA15810.5747001090512541No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT12470.4532897128316975No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA12250.44529262086513993No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12210.44383860414394766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10340.3758633224282079No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9600.3489640130861505No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC9470.34423845874227554No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG9130.33187931661214104No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8470.3078880407124682No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT8190.29770992366412213No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC8060.2929843693202472No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA8010.29116684841875684No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA7770.2824427480916031No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.27735368956743006No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC7460.2711741185023628No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG7300.2653580516175936No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT7220.262450018175209No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT7060.25663395129043987No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA7010.25481643038894947No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG6760.24572882588149766No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC6690.24318429661941113No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT6670.242457288258815No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT6650.24173027989821882No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC6520.2370047255543439No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT6490.23591421301344964No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG6410.23300617957106506No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT6390.23227917121046893No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT6240.22682660850599784No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA6200.2253725917848055No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA6130.222828062522719No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC5980.21737549981824789No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG5820.21155943293347876No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA5770.20974191203198836No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC5750.20901490367139222No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG5590.20319883678662304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5290.19229371137768084No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA5200.1890221737549982No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA5080.1846601235914213No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4880.17739003998545982No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG4830.17557251908396945No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG4760.17302798982188294No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT4750.17266448564158487No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT4720.17157397310069067No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC4710.17121046892039257No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG4510.16394038531443111No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC4510.16394038531443111No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT4390.15957833515085423No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC4390.15957833515085423No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATT4300.15630679752817156No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG4290.1559432933478735No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA4280.15557978916757542No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG4190.15230825154489278No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA4170.1515812431842966No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA4160.15121773900399854No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT4160.15121773900399854No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA4130.15012722646310434No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT4130.15012722646310434No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA4120.14976372228280624No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4100.1490367139222101No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGAT4090.14867320974191203No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTT4000.14540167211922936No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACT3980.14467466375863322No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGT3930.14285714285714285No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG3890.14140312613595057No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC3850.13994910941475827No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTA3800.1381315885132679No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACAT3750.13631406761177753No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT3680.13376953834969102No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGC3660.13304252998909488No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGA3630.13195201744820065No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGA3620.13158851326790258No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTT3610.1312250090876045No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3560.12940748818611414No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATAT3460.1257724463831334No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAA3380.12286441294074883No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA3370.12250090876045074No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA3370.12250090876045074No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA3340.12141039621955652No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTA3290.11959287531806617No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACT3280.11922937113776808No Hit
CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGC3250.11813885859687386No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAAT3240.1177753544165758No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.1148673209741912No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3150.11450381679389314No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGT3140.11414031261359504No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGC3120.11341330425299892No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGA3110.11304980007270082No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.1123227917121047No Hit
GCGGTATCCTGACCGTGCAAAGGTAGCATAATCACTTG3070.11159578335150853No Hit
CTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAA3060.11123227917121048No Hit
TTATTATATTTCAGCCTCTTCACTGAAAGGTCAATTTC3050.11086877499091238No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAA3040.11050527081061431No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACC3030.11014176663031626No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3020.10977826245001816No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGT2980.10832424572882587No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA2970.10796074154852782No Hit
CTATTTAAGAGTTCATATCGACAATTAGGGTTTACGAC2970.10796074154852782No Hit
CGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAA2930.1065067248273355No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGG2920.10614322064703743No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2880.10468920392584516No Hit
TGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACC2860.103962195565249No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATG2850.10359869138495094No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAA2830.10287168302435477No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGG2830.10287168302435477No Hit
CAAGAACCCCGCCTGTTTACCAAAAACATCACCTCTAG2770.10069065794256633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTT200.003747862332.00127432
ATGTCCA200.003747862332.0012749
ATGCTGC308.4074866E-426.662886
ACGCCTA401.5888285E-424.00095621
AACGCCT401.5888285E-424.00095620
AGTGTGC350.00206209322.8580518
AGGACCT350.002064274622.8538975
GTTCTTA653.793266E-722.15472817
ACTCTTA653.793266E-722.15472819
AACTCTT653.793266E-722.15472818
GTGCATT453.5263813E-421.3341811
ACAACCG453.5263813E-421.3341832
TCCTCAA453.5263813E-421.3341813
CAATTAA453.5263813E-421.3341812
TTCCATA453.5263813E-421.3341819
CGGTGTT851.0471922E-820.70670714
TCTTACT707.737199E-720.57224719
TTCTTAC707.737199E-720.57224718
TCTTTGC707.737199E-720.5722478
TTGTTCT707.737199E-720.57224715
Produced by FastQC (version 0.11.5)