FastQCFastQC Report
Thu 2 Feb 2017
SRR4063031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275100
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32351.1759360232642675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23690.861141403126136No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA16130.5863322428207924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15250.5543438749545619No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT13810.5019992729916394No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA12530.45547073791348597No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12520.4551072337331879No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11760.4274809160305344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.3896764812795347No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC10340.3758633224282079No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG8770.3187931661214104No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT8680.3155216284987277No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT7750.28171573973100694No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG7700.27989821882951654No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT7630.27735368956743006No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA7580.27553616866593966No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7560.2748091603053435No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC7470.27153762268266085No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT7460.2711741185023628No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT7350.26717557251908397No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA7340.2668120683387859No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA7300.2653580516175936No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA7170.2606324972737187No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG6990.25408942202835333No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT6840.24863685932388224No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC6820.2479098509632861No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT6820.2479098509632861No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC6710.24391130498000727No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA6560.23845874227553615No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT6550.2380952380952381No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG6250.22719011268629588No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC6070.22064703744093056No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA6050.21992002908033442No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG6010.21846601235914212No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC5910.2148309705561614No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG5610.2039258451472192No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC5480.19920029080334423No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT5200.1890221737549982No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA5080.1846601235914213No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG4890.1777535441657579No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA4870.17702653580516176No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT4810.17484551072337332No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT4790.17411850236277718No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC4760.17302798982188294No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.17193747728098874No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTT4690.17048346055979643No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA4660.16939294801890223No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.16939294801890223No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA4640.16866593965830606No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC4640.16866593965830606No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT4590.1668484187568157No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.16612141039621955No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG4540.16503089785532532No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG4420.16066884769174844No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT4390.15957833515085423No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG4370.1588513267902581No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATT4370.1588513267902581No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT4320.15703380588876772No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA4310.15667030170846966No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGAT4290.1559432933478735No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA4250.15448927662668122No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG4220.15339876408578698No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG4110.14940021810250817No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4030.1464921846601236No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC3990.1450381679389313No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGC3960.14394765539803708No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACAT3950.14358415121773901No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3920.14249363867684478No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA3910.1421301344965467No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC3870.1406761177753544No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTA3780.13740458015267176No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3730.1355870592511814No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTT3670.13340603416939295No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGA3650.1326790258087968No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3640.13231552162849872No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA3620.13158851326790258No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGG3620.13158851326790258No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA3610.1312250090876045No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATAT3580.13013449654671028No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGA3450.12540894220283533No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGC3450.12540894220283533No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA3430.12468193384223918No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGA3360.12213740458015268No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA3360.12213740458015268No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAAT3350.12177390039985461No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGT3340.12141039621955652No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAA3330.12104689203925846No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTA3280.11922937113776808No Hit
TGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACC3260.11850236277717195No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGT3230.1174118502362777No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT3220.11704834605597964No Hit
CAAGAACCCCGCCTGTTTACCAAAAACATCACCTCTAG3200.11632133769538348No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATG3180.11559432933478736No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3170.11523082515448926No Hit
CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGC3140.11414031261359504No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACT3130.11377680843329697No Hit
TTATTATATTTCAGCCTCTTCACTGAAAGGTCAATTTC3130.11377680843329697No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACC3010.1094147582697201No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAA2940.1068702290076336No Hit
AACCTAGACTTACAAGTCAAAGTAAAATCAACATATCT2930.1065067248273355No Hit
CTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT2920.10614322064703743No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTTAAGA2880.10468920392584516No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAA2880.10468920392584516No Hit
AGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATG2860.103962195565249No Hit
CGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAA2840.10323518720465284No Hit
ATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAG2840.10323518720465284No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATT2830.10287168302435477No Hit
TAATCACACTATAAATAATCCACCTATAACTTCTCTGT2810.10214467466375862No Hit
ACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGT2800.10178117048346055No Hit
AAACTATAGTATAAGTTTGAAATTTCGGTTGGGGTGAC2770.10069065794256633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGTA200.003749324731.9987329
TGCGTAT200.003749324731.9987330
TGCCATT308.3935534E-426.6704546
ATTGAAC1050.024.3844158
TGAACTT1050.024.37998210
CTTGTTA552.209108E-623.27180132
GTTCAAA350.002063048322.8562361
GAACTTA1150.022.25998311
AATTCAT1150.022.25998318
TGGGTAA1200.021.33248329
TTGAACT1200.021.3324839
TTCATTT1200.021.33248320
AAATTCA1250.020.47918517
TAAATTC1250.020.47918516
AACTTGC400.00447128720.0028427
GATATAC400.00447599519.9992071
GTAACCA1203.6379788E-1219.99920532
ACCTACT507.1700604E-419.2027268
TACTACC507.1788393E-419.19923611
GTTTAAA1450.018.7578741
Produced by FastQC (version 0.11.5)