FastQCFastQC Report
Thu 2 Feb 2017
SRR4063030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521785
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25280.4844907385225716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18210.3489943175829125No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12450.23860402272966835No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10360.19854921088187663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9860.18896672000919917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.1713349368034727No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7070.13549642093965905No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.11403164138486159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC308.409345E-426.66738710
GTACTAA401.585434E-424.014461
GCATCGT653.807254E-722.15444227
CATCGTT707.765575E-720.57198328
AGGCGTG555.8285623E-520.3622327
TAGGACG555.837169E-520.358334
TATAGAC400.00447955520.000542
ATACCGT801.2656528E-719.994796
TACCGTC802.7920833E-617.998767
GATAACG450.00884260417.77825511
CGATAAC450.00884260417.77825510
CGTCGTA1006.790833E-817.60047510
GCCCTAC550.001357799617.4650611
GTATAGA550.001357799617.4650611
AATCGAG550.001363045817.45501522
CACGCAT753.227478E-517.06712719
ATCGTTT854.972142E-616.94163329
CGTTTAT854.972142E-616.94163331
ACCGTCG854.976684E-616.940018
GCTCGTA1354.4019544E-1016.59303926