##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4063030_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 521785 Sequences flagged as poor quality 0 Sequence length 38 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.104523893940993 32.0 32.0 32.0 32.0 32.0 2 30.721801124984427 32.0 32.0 32.0 32.0 32.0 3 30.80399398219573 32.0 32.0 32.0 32.0 32.0 4 30.845574326590455 32.0 32.0 32.0 32.0 32.0 5 30.76126373889629 32.0 32.0 32.0 32.0 32.0 6 34.404875571356015 36.0 36.0 36.0 32.0 36.0 7 34.29396973849382 36.0 36.0 36.0 32.0 36.0 8 34.27748593769464 36.0 36.0 36.0 32.0 36.0 9 34.41250323409067 36.0 36.0 36.0 32.0 36.0 10 34.13464549575016 36.0 36.0 36.0 32.0 36.0 11 34.44954914380444 36.0 36.0 36.0 32.0 36.0 12 34.25648495069809 36.0 36.0 36.0 32.0 36.0 13 34.33920101191104 36.0 36.0 36.0 32.0 36.0 14 34.24244085207509 36.0 36.0 36.0 32.0 36.0 15 34.192654062497006 36.0 36.0 36.0 32.0 36.0 16 34.21101219851088 36.0 36.0 36.0 32.0 36.0 17 34.14048123269163 36.0 36.0 36.0 32.0 36.0 18 34.16851576798873 36.0 36.0 36.0 32.0 36.0 19 34.14953860306448 36.0 36.0 36.0 32.0 36.0 20 34.12455129986488 36.0 36.0 36.0 32.0 36.0 21 34.09101066531234 36.0 36.0 36.0 32.0 36.0 22 34.07169236371303 36.0 36.0 36.0 32.0 36.0 23 34.01684026945964 36.0 36.0 36.0 32.0 36.0 24 34.005609590156865 36.0 36.0 36.0 32.0 36.0 25 33.98889006008222 36.0 36.0 36.0 32.0 36.0 26 33.93700087200667 36.0 36.0 36.0 32.0 36.0 27 33.96372068955604 36.0 36.0 36.0 32.0 36.0 28 33.94676159720958 36.0 36.0 36.0 32.0 36.0 29 33.889521546230725 36.0 36.0 36.0 32.0 36.0 30 33.86263116034382 36.0 36.0 36.0 32.0 36.0 31 33.86903226424677 36.0 36.0 36.0 32.0 36.0 32 33.829054112325956 36.0 36.0 36.0 32.0 36.0 33 33.762538210182356 36.0 36.0 36.0 32.0 36.0 34 33.751652500550996 36.0 36.0 36.0 32.0 36.0 35 33.68939122435486 36.0 36.0 36.0 27.0 36.0 36 33.654578035014424 36.0 36.0 36.0 27.0 36.0 37 33.64180649117932 36.0 36.0 36.0 27.0 36.0 38 33.02873597362898 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 2.0 8 0.0 9 5.0 10 4.0 11 5.0 12 1.0 13 3.0 14 140.0 15 236.0 16 273.0 17 336.0 18 421.0 19 517.0 20 657.0 21 924.0 22 1308.0 23 1884.0 24 2558.0 25 3865.0 26 5535.0 27 7699.0 28 10931.0 29 14746.0 30 19759.0 31 26456.0 32 35624.0 33 52891.0 34 113788.0 35 221216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.57044120976748 17.697128318091636 11.678032381734523 25.054398090406366 2 15.857687712966994 20.681839536261677 38.360808512351845 25.099664238419482 3 18.739086850937163 25.4674325756661 28.87664763394303 26.916832939453705 4 12.256764966872314 15.96185285059097 36.670442283845915 35.1109398986908 5 13.797855051132268 37.50919920886804 33.76052159514282 14.932424144856876 6 32.540347232470204 36.24169434420056 17.72614286015451 13.49181556317473 7 28.86284580813937 31.503396993014366 21.494676926320228 18.13908027252604 8 27.935421306216007 32.91106247436669 20.082141584033177 19.071374635384124 9 27.696954481077384 14.51120484952557 19.069996531125007 38.72184413827204 10 15.570738625474338 27.29771167925179 32.332783931925334 24.798765763348538 11 36.318406053195446 22.02447803071828 22.643136683698305 19.013979232387967 12 24.305868328200138 24.50404107580587 29.570095464067293 21.619995131926704 13 29.359662187157976 20.284488095854204 25.713568291902188 24.64228142508563 14 23.51124876855788 20.210025567996688 26.33752822254507 29.941197440900364 15 24.86658283365793 27.21288472450122 24.1026928798172 23.817839562023646 16 24.777088620211142 26.421036087493192 24.849156271802382 23.95271902049328 17 23.10446685464656 26.148629350549275 26.34951608914924 24.397387705654925 18 23.823350716904613 25.346090229742963 27.73206684337728 23.09849220997514 19 24.629514631584595 25.81552780269402 26.893059484655428 22.661898081065956 20 24.97335919471937 24.674368862073457 26.823266047731124 23.529005895476047 21 26.22170345624637 24.737662218184536 25.416530107273395 23.6241042182957 22 24.840008663202664 25.068280220106683 25.96622500943944 24.125486107251216 23 23.61204282609279 24.891803932241853 26.53745749782458 24.958695743840778 24 23.75459539444681 26.1673931127544 26.331466424390186 23.746545068408604 25 24.32374928119609 25.069771899559136 26.33084147977765 24.275637339467128 26 23.430401729749622 26.024834481515985 26.926512476662566 23.618251312071827 27 24.311197744466103 25.867723133597448 26.015306503826626 23.80577261810982 28 23.45853162092077 25.425723370368313 27.005954856552545 24.10979015215837 29 23.417611126125347 26.046380578348447 26.956243399325704 23.5797648962005 30 23.74881412129982 26.060391172271043 26.86803445995803 23.322760246471113 31 23.803162434115958 25.928893148059416 25.959367513176808 24.30857690464782 32 23.368222529310184 26.116489409618072 25.955491837998114 24.559796223073636 33 23.282414949688548 25.62817441303306 26.553905126976524 24.53550551030187 34 23.830662396234047 25.92831116656796 26.69422897415698 23.546797463041017 35 24.581950056353364 25.81559033022304 26.522882531991076 23.079577081432525 36 23.671872604042075 26.61890084951084 25.919472812586253 23.789753733860827 37 24.292885441403936 26.438235649268876 25.991455399600945 23.27742350972624 38 23.682662102565775 26.39563164945865 26.061565530049656 23.860140717925916 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 680.0 1 518.0 2 356.0 3 356.0 4 659.0 5 962.0 6 962.0 7 1055.0 8 1148.0 9 1103.5 10 1059.0 11 1059.0 12 1393.5 13 1728.0 14 2018.5 15 2309.0 16 2309.0 17 3231.0 18 4153.0 19 4153.0 20 4740.0 21 5327.0 22 5441.5 23 5556.0 24 5556.0 25 6275.5 26 6995.0 27 6995.0 28 8836.0 29 10677.0 30 12827.0 31 14977.0 32 14977.0 33 17485.0 34 19993.0 35 19993.0 36 21881.5 37 23770.0 38 26637.5 39 29505.0 40 29505.0 41 31816.0 42 34127.0 43 37995.5 44 41864.0 45 41864.0 46 44552.5 47 47241.0 48 47241.0 49 48091.0 50 48941.0 51 47326.5 52 45712.0 53 45712.0 54 43167.5 55 40623.0 56 40623.0 57 38901.0 58 37179.0 59 33150.5 60 29122.0 61 29122.0 62 27136.0 63 25150.0 64 20608.0 65 16066.0 66 16066.0 67 13148.0 68 10230.0 69 10230.0 70 8308.5 71 6387.0 72 4984.0 73 3581.0 74 3581.0 75 2708.5 76 1836.0 77 1836.0 78 1761.5 79 1687.0 80 1324.5 81 962.0 82 962.0 83 921.0 84 880.0 85 880.0 86 572.0 87 264.0 88 258.5 89 253.0 90 253.0 91 179.0 92 105.0 93 93.0 94 81.0 95 81.0 96 73.5 97 66.0 98 66.0 99 149.5 100 233.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12016443554337514 2 0.054428548156807886 3 0.009965790507584543 4 0.0030663970792567823 5 1.916498174535489E-4 6 3.832996349070978E-4 7 0.0 8 5.749494523606466E-4 9 3.832996349070978E-4 10 9.582490872677443E-4 11 0.0021081479919890377 12 0.00344969671416388 13 0.014565386126469716 14 0.007474342880688406 15 0.021656429372251023 16 0.01015744032503809 17 0.018781682110447787 18 0.005366194888699369 19 0.0070910432457813085 20 0.0055578447061529175 21 0.006516093793420661 22 0.007282693063234858 23 0.009007541420316798 24 0.012840537769387775 25 0.016290234483551654 26 0.01744013338827295 27 0.008815891602863248 28 0.004982895253792271 29 0.011115689412305835 30 0.0038329963490709774 31 0.00670774361087421 32 0.007282693063234858 33 0.00670774361087421 34 0.011115689412305835 35 0.014757035943923264 36 0.013990436674109067 37 0.009965790507584543 38 0.006132794158513565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 521785.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.94324401791755 #Duplication Level Percentage of deduplicated Percentage of total 1 85.35662863447814 56.28692990590161 2 9.884866041067935 13.036802668611388 3 2.2867849338265667 4.523940507234682 4 0.8256647629114324 2.177880517506585 5 0.389537297200825 1.2843676521697038 6 0.21925038802787705 0.8674849103247244 7 0.15363659753362868 0.7091906948869866 8 0.11108378646823877 0.5860180190007455 9 0.08321598269733889 0.4938778667881281 >10 0.5471102352028665 7.239411881957057 >50 0.08615050561210312 4.059924213333907 >100 0.05373455018253872 6.844598303345155 >500 0.001168142395272566 0.6127823926972071 >1k 0.001168142395272566 1.276790466242138 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 2528 0.4844907385225716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 1821 0.3489943175829125 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 1245 0.23860402272966835 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 1036 0.19854921088187663 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 986 0.18896672000919917 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 894 0.1713349368034727 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA 707 0.13549642093965905 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 595 0.11403164138486159 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 1.9164981745354887E-4 0.0 16 0.0 0.0 0.0 1.9164981745354887E-4 0.0 17 0.0 0.0 0.0 1.9164981745354887E-4 0.0 18 0.0 0.0 0.0 1.9164981745354887E-4 0.0 19 0.0 0.0 0.0 3.8329963490709774E-4 0.0 20 0.0 0.0 0.0 3.8329963490709774E-4 0.0 21 0.0 0.0 0.0 5.749494523606466E-4 0.0 22 0.0 0.0 0.0 7.665992698141955E-4 0.0 23 0.0 0.0 0.0 0.0032580468967103307 0.0 24 0.0 0.0 0.0 0.0055578447061529175 0.0 25 0.0 0.0 0.0 0.007474342880688406 0.0 26 0.0 0.0 0.0 0.009007541420316798 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 30 8.409345E-4 26.667387 10 GTACTAA 40 1.585434E-4 24.01446 1 GCATCGT 65 3.807254E-7 22.154442 27 CATCGTT 70 7.765575E-7 20.571983 28 AGGCGTG 55 5.8285623E-5 20.362232 7 TAGGACG 55 5.837169E-5 20.35833 4 TATAGAC 40 0.004479555 20.00054 2 ATACCGT 80 1.2656528E-7 19.99479 6 TACCGTC 80 2.7920833E-6 17.99876 7 GATAACG 45 0.008842604 17.778255 11 CGATAAC 45 0.008842604 17.778255 10 CGTCGTA 100 6.790833E-8 17.600475 10 GCCCTAC 55 0.0013577996 17.465061 1 GTATAGA 55 0.0013577996 17.465061 1 AATCGAG 55 0.0013630458 17.455015 22 CACGCAT 75 3.227478E-5 17.067127 19 ATCGTTT 85 4.972142E-6 16.941633 29 CGTTTAT 85 4.972142E-6 16.941633 31 ACCGTCG 85 4.976684E-6 16.94001 8 GCTCGTA 135 4.4019544E-10 16.593039 26 >>END_MODULE