FastQCFastQC Report
Thu 2 Feb 2017
SRR4063030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521785
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31380.6013971271692364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24450.468583803673927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14270.27348428950621423No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13040.24991136195942773No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11670.22365533696829154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11200.21464779554797472No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6010.11518154028958287No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.10885709631361577No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5490.10521574978199834No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.10464080032963768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG401.5925404E-423.99863615
CGAACGA502.7950564E-522.39872616
GTTTAGA604.706906E-621.3362081
CCTAATA453.532266E-421.3341642
TAATACC400.004479245720.0007784
TTTAGAT659.414469E-619.6930752
ATATACG751.5034002E-619.2025896
TTCCGAT507.1822165E-419.2025877
CGATAAC507.1822165E-419.20258710
TAACGAA507.191488E-419.19890813
ATAACGA507.191488E-419.19890812
CTTACAC953.7758582E-818.5270373
TACGCTA802.786659E-618.0024269
GCACCGT450.00883711617.7801746
GCATATA450.00883711617.7801741
ACTAGAC450.00884199717.778473
GATAACG550.001362061117.456911
TCCGATA550.001362061117.45698
CCGATAA550.001362061117.45699
CCATAAG550.001362061117.45691
Produced by FastQC (version 0.11.5)