##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063030_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521785
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.102088024761155	32.0	32.0	32.0	32.0	32.0
2	31.270973676897572	32.0	32.0	32.0	32.0	32.0
3	31.37096121965944	32.0	32.0	32.0	32.0	32.0
4	31.4702492405876	32.0	32.0	32.0	32.0	32.0
5	31.385348371455677	32.0	32.0	32.0	32.0	32.0
6	34.890679111128144	36.0	36.0	36.0	36.0	36.0
7	34.93399196987265	36.0	36.0	36.0	36.0	36.0
8	34.87048880285941	36.0	36.0	36.0	32.0	36.0
9	34.976082102781795	36.0	36.0	36.0	36.0	36.0
10	34.84280498672825	36.0	36.0	36.0	32.0	36.0
11	35.00715812068189	36.0	36.0	36.0	36.0	36.0
12	34.93034870684286	36.0	36.0	36.0	32.0	36.0
13	34.96320515154709	36.0	36.0	36.0	36.0	36.0
14	34.88949663175446	36.0	36.0	36.0	32.0	36.0
15	34.87601790009295	36.0	36.0	36.0	32.0	36.0
16	34.89049321080522	36.0	36.0	36.0	32.0	36.0
17	34.865553820059986	36.0	36.0	36.0	32.0	36.0
18	34.854880841726	36.0	36.0	36.0	32.0	36.0
19	34.83792366587771	36.0	36.0	36.0	32.0	36.0
20	34.81975909617946	36.0	36.0	36.0	32.0	36.0
21	34.806723075596274	36.0	36.0	36.0	32.0	36.0
22	34.79168431442069	36.0	36.0	36.0	32.0	36.0
23	34.733829067527815	36.0	36.0	36.0	32.0	36.0
24	34.71989995879529	36.0	36.0	36.0	32.0	36.0
25	34.70592485410658	36.0	36.0	36.0	32.0	36.0
26	34.61769502764549	36.0	36.0	36.0	32.0	36.0
27	34.61085887865692	36.0	36.0	36.0	32.0	36.0
28	34.58032906273657	36.0	36.0	36.0	32.0	36.0
29	34.54602757840873	36.0	36.0	36.0	32.0	36.0
30	34.506287072261564	36.0	36.0	36.0	32.0	36.0
31	34.50617591536744	36.0	36.0	36.0	32.0	36.0
32	34.45286085265004	36.0	36.0	36.0	32.0	36.0
33	34.40328296137298	36.0	36.0	36.0	32.0	36.0
34	34.37561447722721	36.0	36.0	36.0	32.0	36.0
35	34.3263336431672	36.0	36.0	36.0	32.0	36.0
36	34.28445240855908	36.0	36.0	36.0	32.0	36.0
37	34.272416800022995	36.0	36.0	36.0	32.0	36.0
38	33.8459020477783	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	3.0
21	10.0
22	40.0
23	115.0
24	341.0
25	823.0
26	1834.0
27	3527.0
28	6320.0
29	10060.0
30	15177.0
31	22032.0
32	31402.0
33	49207.0
34	117916.0
35	262977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19943419660518	18.790154407593114	12.406274437646626	25.60413695815508
2	15.162566957654972	21.559262914802073	37.460639918740476	25.817530208802474
3	17.74044865222266	25.941910940329834	29.442969805571263	26.87467060187625
4	11.748539625592198	16.82286156723855	36.594836177422074	34.83376262974717
5	13.474707015341567	37.897026553082206	33.579539465488665	15.048726966087564
6	32.62742317237943	36.65111108981669	17.189838726678612	13.531627011125272
7	28.55814987513919	31.669608513916756	21.63532209873491	18.136919512209147
8	27.759197965398748	33.187803891730695	19.9849359779061	19.06806216496446
9	27.560610997182668	14.180769304482817	18.99035973704889	39.26825996128562
10	15.44566333961946	27.597243303742548	32.28941477699585	24.667678579642153
11	36.40149801823075	21.7860455845261	22.616510399497084	19.195945997746072
12	23.96374745105254	24.776727534765346	29.579864464989342	21.679660549192768
13	29.837385129890663	20.043312858744503	25.640445777475396	24.478856233889438
14	23.432639880410512	20.052512049982273	26.58240463025959	29.932443439347622
15	24.852381728106405	27.223281619824256	23.931121055607193	23.993215596462143
16	24.68334658911237	26.280747817587702	25.006276531521603	24.02962906177832
17	23.05010684477323	26.05536763226233	26.63798307732112	24.25654244564332
18	23.935529001408625	24.95261458263461	28.00597947430455	23.105876941652213
19	24.62968464022538	25.724771697154956	27.103308834098332	22.542234828521327
20	24.84203263796391	24.319403585768086	27.282501413417403	23.5560623628506
21	26.01703766877162	24.678363693858582	25.9375029945284	23.367095642841402
22	24.65881541247832	24.964688521134185	26.376381076497026	24.000114989890474
23	23.412708299395348	24.57410619316385	26.846498078710578	25.16668742873022
24	23.47786923732955	25.910097070632542	26.77597094588767	23.836062746150237
25	24.19722375010301	24.89866477060387	26.684272964048272	24.21983851524484
26	23.5560623628506	25.50034976091685	27.341529557193095	23.60205831903945
27	24.349492607108292	25.350096304033272	26.533725576626388	23.76668551223205
28	23.540155428001956	25.1490556455245	27.320256427455753	23.990532499017796
29	23.450463313433694	25.56167770250199	27.229414413982774	23.758444570081547
30	23.864618568950814	25.29202640934485	27.445978707705283	23.39737631399906
31	23.778759450731624	25.315407687074178	26.48102187682666	24.424810985367536
32	23.36326264649233	25.76003526356641	26.45649070019261	24.42021138974865
33	23.36153779813525	25.233189915386607	26.8773536993206	24.527918587157547
34	23.79255823758828	25.50169130963903	27.262761482219688	23.442988970553007
35	24.714202209722394	25.210958536561996	26.897476930153225	23.177362323562388
36	23.758636219899003	25.991931542685204	26.452082754391178	23.797349483024618
37	24.55302471324396	25.87502515403854	26.38653851682206	23.185411615895436
38	23.69946184628122	25.635320362448827	26.724276712202748	23.940941079067198
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	347.0
1	267.5
2	188.0
3	188.0
4	593.5
5	999.0
6	999.0
7	1164.0
8	1329.0
9	1225.0
10	1121.0
11	1121.0
12	1419.0
13	1717.0
14	2107.5
15	2498.0
16	2498.0
17	3610.0
18	4722.0
19	4722.0
20	5228.5
21	5735.0
22	5671.5
23	5608.0
24	5608.0
25	6260.5
26	6913.0
27	6913.0
28	8765.0
29	10617.0
30	12736.5
31	14856.0
32	14856.0
33	17441.5
34	20027.0
35	20027.0
36	21878.5
37	23730.0
38	26532.0
39	29334.0
40	29334.0
41	31672.0
42	34010.0
43	37555.5
44	41101.0
45	41101.0
46	44560.0
47	48019.0
48	48019.0
49	48726.0
50	49433.0
51	47512.0
52	45591.0
53	45591.0
54	43292.5
55	40994.0
56	40994.0
57	39050.0
58	37106.0
59	33128.5
60	29151.0
61	29151.0
62	27075.0
63	24999.0
64	20505.0
65	16011.0
66	16011.0
67	13175.5
68	10340.0
69	10340.0
70	8321.0
71	6302.0
72	4970.5
73	3639.0
74	3639.0
75	2681.5
76	1724.0
77	1724.0
78	1682.5
79	1641.0
80	1254.5
81	868.0
82	868.0
83	799.5
84	731.0
85	731.0
86	462.0
87	193.0
88	167.0
89	141.0
90	141.0
91	86.0
92	31.0
93	21.0
94	11.0
95	11.0
96	6.0
97	1.0
98	1.0
99	4.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009390841055223894
2	0.0
3	0.0
4	1.916498174535489E-4
5	0.0
6	0.0
7	7.665992698141956E-4
8	0.0022997978094425864
9	0.002874747261803233
10	1.916498174535489E-4
11	0.0055578447061529175
12	1.916498174535489E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	3.832996349070978E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.916498174535489E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	521785.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.057584395342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.36471816290381	54.04200053709454
2	10.566420004263122	13.537186823594299
3	2.4313205797294164	4.672335696844469
4	0.8808459845048441	2.2569946396686866
5	0.39470939911276076	1.26420653226502
6	0.25402734858923587	0.9763426992587966
7	0.16557663344951662	0.7424507419762313
8	0.12360378209554938	0.6334207762535294
9	0.08201669148199989	0.4728412022791443
>10	0.5831058601228691	7.4106078120037155
>50	0.08660039188244947	3.8737499958599106
>100	0.06404820393328797	7.639826036382709
>500	0.0012027831724561035	0.43608819671659693
>1k	0.0018041747586841552	2.0419483098024047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3138	0.6013971271692364	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2445	0.468583803673927	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1427	0.27348428950621423	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1304	0.24991136195942773	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1167	0.22365533696829154	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1120	0.21464779554797472	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	601	0.11518154028958287	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	568	0.10885709631361577	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	549	0.10521574978199834	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.10464080032963768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.8329963490709774E-4	0.0
16	0.0	0.0	0.0	3.8329963490709774E-4	0.0
17	0.0	0.0	0.0	3.8329963490709774E-4	0.0
18	0.0	0.0	0.0	3.8329963490709774E-4	1.9164981745354887E-4
19	0.0	0.0	0.0	5.749494523606466E-4	1.9164981745354887E-4
20	0.0	0.0	0.0	5.749494523606466E-4	1.9164981745354887E-4
21	0.0	0.0	0.0	7.665992698141955E-4	1.9164981745354887E-4
22	0.0	0.0	0.0	9.582490872677443E-4	1.9164981745354887E-4
23	0.0	0.0	0.0	0.00344969671416388	1.9164981745354887E-4
24	0.0	0.0	0.0	0.005749494523606466	1.9164981745354887E-4
25	0.0	0.0	0.0	0.007474342880688406	1.9164981745354887E-4
26	0.0	0.0	0.0	0.009199191237770346	1.9164981745354887E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAACG	40	1.5925404E-4	23.998636	15
CGAACGA	50	2.7950564E-5	22.398726	16
GTTTAGA	60	4.706906E-6	21.336208	1
CCTAATA	45	3.532266E-4	21.334164	2
TAATACC	40	0.0044792457	20.000778	4
TTTAGAT	65	9.414469E-6	19.693075	2
ATATACG	75	1.5034002E-6	19.202589	6
TTCCGAT	50	7.1822165E-4	19.202587	7
CGATAAC	50	7.1822165E-4	19.202587	10
TAACGAA	50	7.191488E-4	19.198908	13
ATAACGA	50	7.191488E-4	19.198908	12
CTTACAC	95	3.7758582E-8	18.527037	3
TACGCTA	80	2.786659E-6	18.002426	9
GCACCGT	45	0.008837116	17.780174	6
GCATATA	45	0.008837116	17.780174	1
ACTAGAC	45	0.008841997	17.77847	3
GATAACG	55	0.0013620611	17.4569	11
TCCGATA	55	0.0013620611	17.4569	8
CCGATAA	55	0.0013620611	17.4569	9
CCATAAG	55	0.0013620611	17.4569	1
>>END_MODULE