FastQCFastQC Report
Thu 2 Feb 2017
SRR4063029_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063029_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502128
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31490.2096359298275513No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25980.17295463502444533No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24760.16483282383392095No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22420.1492549236816037No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22150.14745747366402862No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21890.14572659586932674No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21640.14406229029749795No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21590.1437294291831322No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21360.14219826805704974No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20900.1391359458048848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20550.13680591800432454No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18080.12036257895465634No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17880.11903113449719331No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17770.11829884004558866No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17730.11803255115409605No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17670.11763311781685716No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17520.1166345344737599No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17400.11583566779928209No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA17150.11417136222745333No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16890.1124404844327514No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG16760.11157504553540044No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15670.10431867324222702No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15290.10178892877304731No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15050.10019119542409169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT350.002068784822.8534344
GTACTAA659.3340095E-619.7173371
CTAGCGG5450.018.49425929
TCTAGCG5450.018.49302728
TAGCGGC5600.017.99887730
GTCCTAT5900.016.8349291
ATCGTTT3750.016.63896229
GTATTAG3400.016.4915221
CTATTCC6300.016.2513314
GTATCAA23500.015.9521651
CGAGCCG6150.015.86998515
CCTATTC6400.015.7521643
AGCGGCG6550.015.63261231
AGGCCCG6050.015.60285110
GTATTAA1652.910383E-1115.5348711
CGAACGA2400.015.33339816
GTTCAAA6900.015.091621
CATCGTT4250.015.05688228
TCGTTTA4150.015.03520530
CGGTCCA7250.014.56509310
Produced by FastQC (version 0.11.5)