##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063029_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502128
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257371542238744	32.0	32.0	32.0	32.0	32.0
2	30.855363857141334	32.0	32.0	32.0	32.0	32.0
3	30.90112427169988	32.0	32.0	32.0	32.0	32.0
4	30.984379493625045	32.0	32.0	32.0	32.0	32.0
5	30.867008670366307	32.0	32.0	32.0	32.0	32.0
6	34.532915304155175	36.0	36.0	36.0	32.0	36.0
7	34.469274256255126	36.0	36.0	36.0	32.0	36.0
8	34.45514496767253	36.0	36.0	36.0	32.0	36.0
9	34.61694742392126	36.0	36.0	36.0	32.0	36.0
10	34.30849701223864	36.0	36.0	36.0	32.0	36.0
11	34.587535150133675	36.0	36.0	36.0	32.0	36.0
12	34.39395644046313	36.0	36.0	36.0	32.0	36.0
13	34.49697229530373	36.0	36.0	36.0	32.0	36.0
14	34.38926975597286	36.0	36.0	36.0	32.0	36.0
15	34.31448252079716	36.0	36.0	36.0	32.0	36.0
16	34.3373780396877	36.0	36.0	36.0	32.0	36.0
17	34.25457817176699	36.0	36.0	36.0	32.0	36.0
18	34.28554890129203	36.0	36.0	36.0	32.0	36.0
19	34.27923053161914	36.0	36.0	36.0	32.0	36.0
20	34.26825343778959	36.0	36.0	36.0	32.0	36.0
21	34.25387317192676	36.0	36.0	36.0	32.0	36.0
22	34.22924278090815	36.0	36.0	36.0	32.0	36.0
23	34.17231687312932	36.0	36.0	36.0	32.0	36.0
24	34.15310213244144	36.0	36.0	36.0	32.0	36.0
25	34.13030114610739	36.0	36.0	36.0	32.0	36.0
26	34.07441176783869	36.0	36.0	36.0	32.0	36.0
27	34.09519694726415	36.0	36.0	36.0	32.0	36.0
28	34.071636371867115	36.0	36.0	36.0	32.0	36.0
29	34.043713318705194	36.0	36.0	36.0	32.0	36.0
30	34.02031251664305	36.0	36.0	36.0	32.0	36.0
31	34.03964975022102	36.0	36.0	36.0	32.0	36.0
32	33.99781643108976	36.0	36.0	36.0	32.0	36.0
33	33.9554385511754	36.0	36.0	36.0	32.0	36.0
34	33.96432527720673	36.0	36.0	36.0	32.0	36.0
35	33.9285400445235	36.0	36.0	36.0	32.0	36.0
36	33.88447322731485	36.0	36.0	36.0	32.0	36.0
37	33.89124695099219	36.0	36.0	36.0	32.0	36.0
38	33.28922235655018	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	6.0
10	11.0
11	11.0
12	3.0
13	8.0
14	153.0
15	391.0
16	476.0
17	552.0
18	684.0
19	854.0
20	1190.0
21	1804.0
22	2685.0
23	4199.0
24	6403.0
25	9902.0
26	14115.0
27	20599.0
28	28826.0
29	38900.0
30	52686.0
31	71819.0
32	99049.0
33	148813.0
34	329424.0
35	668560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.83471083193806	17.103533683840073	11.196930755366912	25.864824728854952
2	17.33693628647607	19.10713013059484	36.55330063855396	27.002632944375137
3	19.044261527778055	23.040775413976014	28.107025557954646	29.80793750029129
4	12.76000404768816	14.58519185242981	35.30622624979029	37.34857785009174
5	15.205935062618	35.916209314277644	33.14899435732336	15.728861265780989
6	35.520855108026886	34.86238837487667	16.128939915825967	13.487816601270467
7	31.259607210309113	29.781702878651405	20.428499055006665	18.530190856032814
8	28.95326883799207	31.99549437995798	19.089169735654313	19.96206704639564
9	27.398984771573602	13.869152034617624	18.48434717483565	40.24751601897312
10	16.46226666311613	25.598820333928046	30.60601017228983	27.33290283066599
11	38.556784350484385	20.661285769826858	21.608423917385213	19.17350596230354
12	25.219060274362924	23.34208553636314	27.773420908622477	23.665433280651463
13	29.417380964111793	18.40583724271643	25.33566542516289	26.841116368008883
14	24.03308093871358	19.31887407099672	24.156448712494498	32.491596277795196
15	25.649734819101017	26.584543266269588	21.700020974760374	26.065700939869025
16	26.81062302303022	25.142295219053747	22.770497402433982	25.27658435548205
17	24.713151103049867	25.515369188113752	24.40665958200496	25.36482012683143
18	26.073097238847602	23.806134799414668	25.355013867677194	24.765754094060533
19	26.09772449317228	24.410218376486895	24.772659032155623	24.7193980981852
20	26.1972462985611	23.568773630189654	24.233864229643334	26.000115841605908
21	27.79339716161435	23.565711150952932	23.80351932124808	24.83737236618464
22	26.522905667663093	23.311185528917534	24.607298123206593	25.55861068021278
23	24.679863170383232	23.149717645774274	25.372932609150112	26.797486574692382
24	25.37895659850874	24.4665445589842	24.58399164288097	25.570507199626086
25	25.47945424377928	23.782754827833248	24.7050434245324	26.032747503855074
26	25.240450078601956	24.930503352152083	25.178660484156097	24.650386085089863
27	26.154570312161912	23.92176078838409	24.180281210032696	25.743387689421304
28	25.041942399105228	23.79405616286966	25.467358161458264	25.69664327656685
29	24.91641111748782	24.26560958299434	25.39863674555291	25.41934255396493
30	24.742242647083298	24.58519490496914	25.526948766752483	25.14561368119508
31	25.655016673756926	24.336494989218146	24.029649927666053	25.978838409358872
32	25.361091045874122	24.08815980879401	23.83936566481031	26.711383480521555
33	24.857375496081612	23.84953339533868	24.75877501046923	26.534316098110477
34	26.120353485015237	23.95165247520072	24.776575126218493	25.15141891356555
35	26.606370151094172	23.62510162053544	25.00041613650875	24.768112091861635
36	24.9948566417899	24.626732013393372	24.383446176504076	25.99496516831265
37	26.41537572469847	24.225101930487536	24.339216140569533	25.020306204244463
38	25.177224223928334	23.878001560531857	24.95313078375449	25.991643431785317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	115.5
2	144.0
3	144.0
4	381.5
5	619.0
6	619.0
7	747.5
8	876.0
9	885.0
10	894.0
11	894.0
12	1177.5
13	1461.0
14	2037.0
15	2613.0
16	2613.0
17	3884.5
18	5156.0
19	5156.0
20	6342.0
21	7528.0
22	8061.0
23	8594.0
24	8594.0
25	10060.5
26	11527.0
27	11527.0
28	14892.0
29	18257.0
30	22460.5
31	26664.0
32	26664.0
33	35468.5
34	44273.0
35	44273.0
36	49612.0
37	54951.0
38	64349.0
39	73747.0
40	73747.0
41	79784.5
42	85822.0
43	100056.5
44	114291.0
45	114291.0
46	116617.0
47	118943.0
48	118943.0
49	129187.5
50	139432.0
51	144014.0
52	148596.0
53	148596.0
54	142212.0
55	135828.0
56	135828.0
57	132812.5
58	129797.0
59	117077.0
60	104357.0
61	104357.0
62	100104.5
63	95852.0
64	79808.0
65	63764.0
66	63764.0
67	53293.0
68	42822.0
69	42822.0
70	34347.0
71	25872.0
72	19808.5
73	13745.0
74	13745.0
75	10354.0
76	6963.0
77	6963.0
78	6793.5
79	6624.0
80	5324.0
81	4024.0
82	4024.0
83	3967.5
84	3911.0
85	3911.0
86	2609.5
87	1308.0
88	1140.0
89	972.0
90	972.0
91	712.0
92	452.0
93	399.0
94	346.0
95	346.0
96	309.5
97	273.0
98	273.0
99	508.0
100	743.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12142773452062673
2	0.051060894943706525
3	0.011250705665562456
4	0.0023966000234334223
5	2.662888914926025E-4
6	1.3314444574630125E-4
7	6.657222287315062E-5
8	2.662888914926025E-4
9	1.997166686194519E-4
10	5.32577782985205E-4
11	0.001597733348955615
12	0.0019971666861945186
13	0.012382433454406015
14	0.007655805630412322
15	0.02150282798802765
16	0.00965297231660684
17	0.01943908907895998
18	0.004926344492613146
19	0.005924927835710405
20	0.005126061161232598
21	0.005126061161232598
22	0.006524077841568761
23	0.007589233407539171
24	0.011650139002801358
25	0.014113311249107932
26	0.017109061278399708
27	0.007922094521904925
28	0.004526911155374242
29	0.008920677865002183
30	0.002463172246306573
31	0.004460338932501091
32	0.0055254944984715025
33	0.007123227847427116
34	0.011250705665562456
35	0.014446172363473685
36	0.01271529456877177
37	0.008254955636270676
38	0.0050594889383594474
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1502128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.901468987470004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.93399505835403	38.11086842415741
2	12.70849657456706	12.429283022371198
3	3.9198786306610947	5.750634698757597
4	1.7577862279780552	3.4383331485628306
5	0.972490760593371	2.3778113384878927
6	0.576681211646579	1.6920335032195088
7	0.39523047359455493	1.352914552317105
8	0.27276949718719823	1.0671063285942048
9	0.20408598640437192	0.8982094081437557
>10	1.0567994969242815	9.191179025238608
>50	0.08708708480564223	3.0116196818083605
>100	0.09214203924197427	9.98539119472964
>500	0.015448098537693874	5.2131680360326955
>1k	0.007108859504071518	5.481447637579236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3149	0.2096359298275513	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2598	0.17295463502444533	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2476	0.16483282383392095	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2242	0.1492549236816037	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2215	0.14745747366402862	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2189	0.14572659586932674	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2164	0.14406229029749795	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2159	0.1437294291831322	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2136	0.14219826805704974	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2090	0.1391359458048848	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2055	0.13680591800432454	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1808	0.12036257895465634	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1788	0.11903113449719331	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1777	0.11829884004558866	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1773	0.11803255115409605	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1767	0.11763311781685716	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1752	0.1166345344737599	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1740	0.11583566779928209	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1715	0.11417136222745333	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1689	0.1124404844327514	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1676	0.11157504553540044	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1567	0.10431867324222702	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1529	0.10178892877304731	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1505	0.10019119542409169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.657222287315062E-5	6.657222287315062E-5
2	0.0	0.0	0.0	1.3314444574630125E-4	1.3314444574630125E-4
3	0.0	0.0	0.0	1.9971666861945186E-4	1.3314444574630125E-4
4	0.0	0.0	0.0	2.662888914926025E-4	1.3314444574630125E-4
5	0.0	0.0	0.0	2.662888914926025E-4	1.9971666861945186E-4
6	0.0	0.0	0.0	2.662888914926025E-4	2.662888914926025E-4
7	0.0	0.0	0.0	2.662888914926025E-4	2.662888914926025E-4
8	0.0	0.0	0.0	2.662888914926025E-4	2.662888914926025E-4
9	0.0	0.0	0.0	3.994333372389037E-4	3.3286111436575314E-4
10	0.0	0.0	0.0	3.994333372389037E-4	3.3286111436575314E-4
11	0.0	0.0	0.0	3.994333372389037E-4	3.3286111436575314E-4
12	0.0	0.0	0.0	3.994333372389037E-4	5.32577782985205E-4
13	0.0	0.0	0.0	3.994333372389037E-4	5.32577782985205E-4
14	0.0	0.0	0.0	3.994333372389037E-4	6.657222287315063E-4
15	0.0	0.0	0.0	3.994333372389037E-4	7.322944516046569E-4
16	0.0	0.0	0.0	3.994333372389037E-4	7.322944516046569E-4
17	0.0	0.0	0.0	4.6600556011205436E-4	7.322944516046569E-4
18	0.0	0.0	0.0	5.991500058583556E-4	9.985833430972593E-4
19	0.0	0.0	0.0	6.657222287315063E-4	9.985833430972593E-4
20	0.0	0.0	0.0	7.988666744778074E-4	0.00106515556597041
21	0.0	0.0	0.0	7.988666744778074E-4	0.0011317277888435607
22	0.0	0.0	0.0	9.320111202241087E-4	0.0011317277888435607
23	0.0	0.0	0.0	9.985833430972593E-4	0.001398016680336163
24	0.0	0.0	0.0	0.001398016680336163	0.001398016680336163
25	0.0	0.0	0.0	0.0019305944633213681	0.001398016680336163
26	0.0	0.0	0.0	0.002596316692052874	0.0015311611260824644
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCGT	35	0.0020687848	22.853434	4
GTACTAA	65	9.3340095E-6	19.717337	1
CTAGCGG	545	0.0	18.494259	29
TCTAGCG	545	0.0	18.493027	28
TAGCGGC	560	0.0	17.998877	30
GTCCTAT	590	0.0	16.834929	1
ATCGTTT	375	0.0	16.638962	29
GTATTAG	340	0.0	16.491522	1
CTATTCC	630	0.0	16.251331	4
GTATCAA	2350	0.0	15.952165	1
CGAGCCG	615	0.0	15.869985	15
CCTATTC	640	0.0	15.752164	3
AGCGGCG	655	0.0	15.632612	31
AGGCCCG	605	0.0	15.602851	10
GTATTAA	165	2.910383E-11	15.534871	1
CGAACGA	240	0.0	15.333398	16
GTTCAAA	690	0.0	15.09162	1
CATCGTT	425	0.0	15.056882	28
TCGTTTA	415	0.0	15.035205	30
CGGTCCA	725	0.0	14.565093	10
>>END_MODULE