FastQCFastQC Report
Thu 2 Feb 2017
SRR4063029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502128
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34200.22767700222617515No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27270.18154245177508174No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26430.17595038505373709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23670.15757645154074754No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23560.15684415708914287No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23330.1553129959630604No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22740.15138523481354452No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21640.14406229029749795No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21030.14000138470223578No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20870.13893622913626535No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20580.13700563467294397No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20490.13640648466708563No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18850.12548864011588892No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18470.1229588956467092No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18060.12022943450891004No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18030.12002971784029057No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18000.11983000117167111No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17330.11536966223917003No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG17250.11483708445618483No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17080.11370535666734127No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG16100.10718127882577251No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16020.1066487010427873No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15640.10411895657360756No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT15490.1031203732305103No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15060.10025776764696484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC953.790774E-818.5266821
TCTAGCG4650.017.54756528
AAGACGG6350.017.1353385
CAAGACG6450.017.1166174
CGAGCCG6250.016.8952115
GTATCAA24500.016.7186991
CTAGCGG4950.016.48407629
AGGCCCG6550.016.36782510
TAGCGGC5000.016.31923530
ATAGTGC1203.1073796E-816.0019158
ATTAGAC703.6977217E-415.9997843
CTTATAC1001.3152094E-615.9997843
ACCGTCG5150.015.8465568
TAAACGC4250.015.81102428
GTTCAAA7050.015.6598841
GTTATTC3600.015.55534653
CTTACAC1551.891749E-1015.4836633
TAATACC1451.2205419E-915.4485814
CGCTTCG4500.015.28868232
CCCTATA1052.1869782E-615.2373812
Produced by FastQC (version 0.11.5)