##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063029_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502128
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20680195029984	32.0	32.0	32.0	32.0	32.0
2	31.269868479916493	32.0	32.0	32.0	32.0	32.0
3	31.36172150442572	32.0	32.0	32.0	32.0	32.0
4	31.462174994407935	32.0	32.0	32.0	32.0	32.0
5	31.37896104726095	32.0	32.0	32.0	32.0	32.0
6	34.92247997507536	36.0	36.0	36.0	36.0	36.0
7	34.95423026533025	36.0	36.0	36.0	36.0	36.0
8	34.87888116059351	36.0	36.0	36.0	32.0	36.0
9	35.0006510763397	36.0	36.0	36.0	36.0	36.0
10	34.83524240277792	36.0	36.0	36.0	32.0	36.0
11	35.02044832397772	36.0	36.0	36.0	36.0	36.0
12	34.91001832067573	36.0	36.0	36.0	32.0	36.0
13	34.97244841984172	36.0	36.0	36.0	36.0	36.0
14	34.90575303835625	36.0	36.0	36.0	32.0	36.0
15	34.88423157014582	36.0	36.0	36.0	32.0	36.0
16	34.8885547702992	36.0	36.0	36.0	32.0	36.0
17	34.847140190449814	36.0	36.0	36.0	32.0	36.0
18	34.84850292385203	36.0	36.0	36.0	32.0	36.0
19	34.852271577388876	36.0	36.0	36.0	32.0	36.0
20	34.83616975384255	36.0	36.0	36.0	32.0	36.0
21	34.83050046334267	36.0	36.0	36.0	32.0	36.0
22	34.824872447620976	36.0	36.0	36.0	32.0	36.0
23	34.76190843922755	36.0	36.0	36.0	32.0	36.0
24	34.73496333201964	36.0	36.0	36.0	32.0	36.0
25	34.71139210506694	36.0	36.0	36.0	32.0	36.0
26	34.65440029078747	36.0	36.0	36.0	32.0	36.0
27	34.63506505437619	36.0	36.0	36.0	32.0	36.0
28	34.61195117859464	36.0	36.0	36.0	32.0	36.0
29	34.57537906223704	36.0	36.0	36.0	32.0	36.0
30	34.54694207151454	36.0	36.0	36.0	32.0	36.0
31	34.5486163629198	36.0	36.0	36.0	32.0	36.0
32	34.5212125730963	36.0	36.0	36.0	32.0	36.0
33	34.49143015774954	36.0	36.0	36.0	32.0	36.0
34	34.48464445107208	36.0	36.0	36.0	32.0	36.0
35	34.460176496277285	36.0	36.0	36.0	32.0	36.0
36	34.42725786351097	36.0	36.0	36.0	32.0	36.0
37	34.43192590777883	36.0	36.0	36.0	32.0	36.0
38	33.99368895327162	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	27.0
22	98.0
23	369.0
24	933.0
25	2353.0
26	5025.0
27	9619.0
28	16865.0
29	27207.0
30	41145.0
31	60374.0
32	87524.0
33	141348.0
34	346260.0
35	762976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29176423122017	18.252366682490045	11.874657539262728	26.581211547027056
2	16.32297647071355	20.091962868676973	35.828837489215296	27.756223171394183
3	18.071895337814087	23.742983287709173	28.455963806013866	29.72915756846287
4	12.184722064113087	15.485641360550739	35.53501135390017	36.79462522143601
5	14.701676554860837	36.397896850334995	32.817442987548326	16.08298360725584
6	35.514730455367925	35.21988168768798	15.81265486383965	13.45273299310444
7	30.99184887532587	30.097049244935835	20.360236571681167	18.550865308057123
8	28.781905332534453	32.30164436455629	18.86505558884229	20.051394714066973
9	27.306596114246457	13.676832544301092	18.298646317601733	40.71792502385072
10	16.212108502794052	25.877660743030837	30.51737613156889	27.39285462260621
11	38.723675371805314	20.687614301131177	21.48784704769374	19.100863279369772
12	25.1079640055628	23.37102004432399	27.664615566921707	23.8564003831915
13	29.390716079364953	18.468848111074717	25.33725577915006	26.803180030410275
14	23.936908172938658	19.232515471384595	24.153534186167892	32.67704216950886
15	25.51999563286218	26.738666744778072	21.56474015529968	26.176597467060063
16	26.84924320697038	25.254106174706813	22.608592609950684	25.288058008372122
17	24.737838586325534	25.600082016978583	24.195474686577974	25.466604710117913
18	25.996652748633934	23.958610717595306	25.363084903550163	24.681651630220593
19	26.041389282404698	24.52161200643354	24.708746524929968	24.7282521862318
20	26.217805673018546	23.472034340615448	24.221903859058617	26.088256127307393
21	27.646282668697562	23.712857643100513	23.738554555343562	24.902305132858363
22	26.426744494136976	23.471133580005937	24.52091236021479	25.5812095656423
23	24.546242520590845	23.214661372829408	25.311225970692163	26.927870135887584
24	25.406489992863456	24.45404120021729	24.553633245635524	25.585835561283726
25	25.410368098036983	23.81882490628289	24.70390319859772	26.066903797082404
26	25.35904088003211	24.890571835803495	25.115519526644555	24.63486775751984
27	26.169691377626524	23.93046659836352	24.152085675844987	25.74775634816497
28	24.995872522181863	23.93164896733168	25.428192537520104	25.64428597296635
29	25.039610472609525	24.12750444702449	25.367145809145423	25.46573927122056
30	24.81599437597861	24.464892472545614	25.533776082996923	25.18533706847885
31	25.732361023827533	24.28175228742158	24.052677268515065	25.933209420235826
32	25.47492623797706	24.031107868304165	23.95994216205277	26.53402373166601
33	24.912657243590427	23.72520850420204	24.775451892248864	26.586682359958676
34	26.066620154873622	23.83125805523897	24.871515609854818	25.230606180032595
35	26.661576110690966	23.58787000841473	24.994940511061642	24.755613369832663
36	25.13920251802776	24.563352790175006	24.34433017692234	25.953114514874898
37	26.475440175537635	24.278823109615157	24.300059648711695	24.94567706613551
38	25.21572757544973	23.79627274942315	24.9266705988712	26.06132907625592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	51.0
2	75.0
3	75.0
4	272.5
5	470.0
6	470.0
7	653.5
8	837.0
9	818.0
10	799.0
11	799.0
12	1093.0
13	1387.0
14	2031.5
15	2676.0
16	2676.0
17	4001.0
18	5326.0
19	5326.0
20	6426.5
21	7527.0
22	8125.5
23	8724.0
24	8724.0
25	10221.5
26	11719.0
27	11719.0
28	14904.0
29	18089.0
30	22298.0
31	26507.0
32	26507.0
33	35655.5
34	44804.0
35	44804.0
36	50189.0
37	55574.0
38	65034.5
39	74495.0
40	74495.0
41	80442.5
42	86390.0
43	100258.0
44	114126.0
45	114126.0
46	116516.0
47	118906.0
48	118906.0
49	129317.5
50	139729.0
51	143954.0
52	148179.0
53	148179.0
54	142488.5
55	136798.0
56	136798.0
57	133235.0
58	129672.0
59	117361.0
60	105050.0
61	105050.0
62	100643.0
63	96236.0
64	79593.5
65	62951.0
66	62951.0
67	52973.0
68	42995.0
69	42995.0
70	34420.0
71	25845.0
72	19911.0
73	13977.0
74	13977.0
75	10346.0
76	6715.0
77	6715.0
78	6759.0
79	6803.0
80	5272.5
81	3742.0
82	3742.0
83	3530.5
84	3319.0
85	3319.0
86	2115.5
87	912.0
88	734.0
89	556.0
90	556.0
91	332.5
92	109.0
93	74.0
94	39.0
95	39.0
96	30.5
97	22.0
98	22.0
99	21.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008720961196382732
2	0.0
3	0.0
4	6.657222287315062E-5
5	0.0
6	1.3314444574630125E-4
7	2.662888914926025E-4
8	0.0018640222404482174
9	0.005126061161232598
10	6.657222287315062E-4
11	0.0043271944867547905
12	4.6600556011205436E-4
13	2.662888914926025E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3314444574630125E-4
22	1.3314444574630125E-4
23	2.662888914926025E-4
24	0.0
25	6.657222287315062E-5
26	6.657222287315062E-5
27	6.657222287315062E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.3314444574630125E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1502128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.78439101124503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2697421107236	35.682334372349146
2	13.577839298728902	12.704618856791642
3	4.308312751534956	6.046853650996329
4	1.8661298439911163	3.492229931961363
5	1.0227002630404651	2.3923204496694135
6	0.6295248199045123	1.7671161195417788
7	0.4268614195385044	1.3979316091513152
8	0.30735579309866046	1.1503562883119247
9	0.23054953901218647	0.9707507802547579
>10	1.1448847170575849	9.593546674275066
>50	0.09046405645835807	2.9578454761071247
>100	0.09976512423479	10.102878389713386
>500	0.017580344249710732	5.645327861285198
>1k	0.008289918426692867	6.0958895395915995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3420	0.22767700222617515	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2727	0.18154245177508174	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2643	0.17595038505373709	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2367	0.15757645154074754	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2356	0.15684415708914287	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2333	0.1553129959630604	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2274	0.15138523481354452	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2164	0.14406229029749795	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2103	0.14000138470223578	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2087	0.13893622913626535	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2058	0.13700563467294397	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2049	0.13640648466708563	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1885	0.12548864011588892	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1847	0.1229588956467092	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1806	0.12022943450891004	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1803	0.12002971784029057	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1800	0.11983000117167111	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1733	0.11536966223917003	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1725	0.11483708445618483	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1708	0.11370535666734127	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1610	0.10718127882577251	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1602	0.1066487010427873	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1564	0.10411895657360756	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1549	0.1031203732305103	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1506	0.10025776764696484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.657222287315062E-5
2	0.0	0.0	0.0	0.0	6.657222287315062E-5
3	0.0	0.0	0.0	0.0	6.657222287315062E-5
4	0.0	0.0	0.0	0.0	1.3314444574630125E-4
5	0.0	0.0	0.0	0.0	1.3314444574630125E-4
6	0.0	0.0	0.0	0.0	3.3286111436575314E-4
7	0.0	0.0	0.0	0.0	3.3286111436575314E-4
8	0.0	0.0	0.0	0.0	3.3286111436575314E-4
9	0.0	0.0	0.0	6.657222287315062E-5	3.994333372389037E-4
10	0.0	0.0	0.0	6.657222287315062E-5	5.32577782985205E-4
11	0.0	0.0	0.0	6.657222287315062E-5	5.32577782985205E-4
12	0.0	0.0	0.0	6.657222287315062E-5	7.322944516046569E-4
13	0.0	0.0	0.0	6.657222287315062E-5	8.654388973509581E-4
14	0.0	0.0	0.0	6.657222287315062E-5	9.320111202241087E-4
15	0.0	0.0	0.0	6.657222287315062E-5	9.985833430972593E-4
16	0.0	0.0	0.0	1.3314444574630125E-4	0.00106515556597041
17	0.0	0.0	0.0	2.662888914926025E-4	0.0011317277888435607
18	0.0	0.0	0.0	3.3286111436575314E-4	0.0011317277888435607
19	0.0	0.0	0.0	5.991500058583556E-4	0.0011317277888435607
20	0.0	0.0	0.0	5.991500058583556E-4	0.0012648722345898619
21	0.0	0.0	0.0	6.657222287315063E-4	0.0013314444574630126
22	0.0	0.0	0.0	9.320111202241087E-4	0.0013314444574630126
23	0.0	0.0	0.0	0.00106515556597041	0.001398016680336163
24	0.0	0.0	0.0	0.0013314444574630126	0.0014645889032093137
25	0.0	0.0	0.0	0.0015977333489556149	0.0015311611260824644
26	0.0	0.0	0.0	0.0022634555776871214	0.0015311611260824644
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	95	3.790774E-8	18.526682	1
TCTAGCG	465	0.0	17.547565	28
AAGACGG	635	0.0	17.135338	5
CAAGACG	645	0.0	17.116617	4
CGAGCCG	625	0.0	16.89521	15
GTATCAA	2450	0.0	16.718699	1
CTAGCGG	495	0.0	16.484076	29
AGGCCCG	655	0.0	16.367825	10
TAGCGGC	500	0.0	16.319235	30
ATAGTGC	120	3.1073796E-8	16.001915	8
ATTAGAC	70	3.6977217E-4	15.999784	3
CTTATAC	100	1.3152094E-6	15.999784	3
ACCGTCG	515	0.0	15.846556	8
TAAACGC	425	0.0	15.811024	28
GTTCAAA	705	0.0	15.659884	1
GTTATTC	360	0.0	15.5553465	3
CTTACAC	155	1.891749E-10	15.483663	3
TAATACC	145	1.2205419E-9	15.448581	4
CGCTTCG	450	0.0	15.288682	32
CCCTATA	105	2.1869782E-6	15.237381	2
>>END_MODULE