FastQCFastQC Report
Thu 2 Feb 2017
SRR4063028_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063028_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130140
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37820.33464880457288476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26190.23174120020528433No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22650.20041764737112217No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20850.18449041711646344No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17440.15431716424513778No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17140.15166262586936133No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17110.15139717203178366No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16690.14768081830569663No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16520.14617657989275665No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16340.14458385686729078No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16130.14272568000424726No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14770.13069177270072735No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14610.12927601890031323No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14460.127948749712425No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14330.12679844974958854No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14280.12635602668695914No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13760.12175482683561328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13570.12007361919762152No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13490.11936574229741448No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13150.1163572654715345No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC12830.11352575787070629No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC12760.11290636558302511No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT12640.11184455023271454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.1067124427062134No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11940.1056506273559028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3950.019.8470628
CTAGCGG4100.019.12094729
TAGCGGC4100.018.73155230
GTATTAG3000.018.6797391
AAGACGG5350.018.53785
CAAGACG5400.018.3669664
GACGGAC5250.018.2839457
CGCAAGA5600.018.0046312
GCTATAT450.00885963417.7744833
AGCGGCG4450.017.25828431
GCGCAAG5600.017.1548611
CGCTTCG3000.017.06652632
ATAATAC753.2387587E-517.0635053
AAACGCT3100.017.03136329
ACAATAC2450.016.9764443
GTCCTAT5400.016.9007151
ACGGACC5600.016.855518
AGAGCGA5800.016.82819215
CTATTCC5050.016.7889694
GCAAGAC6400.016.7468973
Produced by FastQC (version 0.11.5)